Regulation of chondrocyte differentiation through G protein signal

• Project/Area Number: 22390286
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: TAKESHITA Katsushi 東京大学, 大学院・医学系研究科, 准教授 (30262009)
• Co-Investigator(Kenkyū-buntansha): OGATA Naoshi 東京大学, 大学院・医学系研究科, 講師 (10361495) ; NAKAMURA Kozo 国立障害者リハビリテーションセンター, 自立支援局, 局長 (60126133) ; 三浦 俊樹 東京大学, 医学部附属病院, 講師 (20376479)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 軟骨代謝 / G 蛋白 / 細胞・組織 / G蛋白 / 骨軟骨代謝

Research Abstract

PTHrP signal through both G alpha s and G alpha q play important roles in regulation of chondrocyte differentiation, although a precise mechanism is not clear. In this study, we have tried to establish mouse model of both overexpression of either G alpha s or G alpha q in chondrocyte and also establish conditional knock-out mice of either G alpha s or G alpha q in chondrocytes to explore the specific roles of G alpha s and G alpha q in chondrocytes. Moreover, we have explored to find any proteins that interact directly with PTHrP receptor or downstream of its signal and examined any involvement in chondrocyte differentiation.

Research Products

• [Journal Article] β-catenin regulates parathyroidhormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor (2013)
  • Author(s): Yano F, Saito T, Ogata N, Yamazawa T,Iino M, Chung UI, Kawaguchi H
  • Journal Title: Arthritis Rheum Volume: 65 Pages: 429-35
  • Peer Reviewed
• [Journal Article] β-catenin regulates PTH/PTHrP receptor signals and chondrocyte hypertrophy through binding to its intracellular C-terminal region (2013)
  • Author(s): Yano, F., Saito, T., Ogata, N., Yamazawa, T., Iino, M., Chung, U.I. and Kawaguchi, H
  • Journal Title: Arthritis Rheum Volume: 65 Issue: 2 Pages: 429-435
  • DOI: 10.1002/art.37779
  • Peer Reviewed
• [Journal Article] G alpha(q) signal in osteoblasts is inhibitory to the osteoanabolic action of parathyroid hormone (2011)
  • Author(s): Ogata N, Shinoda Y, Wettschureck N, Offermanns S, Takeda S, Nakamura K, Segre GV, Chung UI, Kawaguchi H
  • Journal Title: J Biol Chem Volume: 286 Pages: 13733-40
  • Peer Reviewed
• [Journal Article] G{alpha}q Signal in Osteoblasts Is Inhibitory to the Osteoanabolic Action of Parathyroid Hormone (2011)
  • Author(s): Ogata, N., et al
  • Journal Title: Jounal of Biological Chemistry Volume: 286 Pages: 13733-40
  • Peer Reviewed
• [Presentation] PTHによる骨形成促進作用の分子メカニズム (2010)
  • Author(s): 緒方直史
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-07-21
• [Presentation] PTHによる骨形成促進作用の分子メカニズム (2010)
  • Author(s): 緒方直史
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-21