Identification of transcription factors that regulate tendon/ligament formation

• Project/Area Number: 22390289
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: SHUKUNAMI Chisa 京都大学, 再生医科学研究所, 准教授 (60303905)
• Research Collaborator: SUGIMOTO Yuki 京都大学, 再生医科学研究所, 研究生 ; SHIMAMURA Satoko 京都大学, 再生医科学研究所, 大学院生
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 転写因子 / Tenomodulin / 腱 / 靱帯 / 運動器 / enhancer / 靭帯 / tenomodulin

Research Abstract

Mouse Tenomodulin (Tnmd)is predominantly expressed in dense connective tissues such as tendons and ligaments. We identified the enhancer regions near the promoter and within the intron 2 of the mouseTnmdgene. We found that these enhancer regionsare transactivated by the transcription factors of basic helix-loop-helix, Ets, or Sox family.

Research Products

• [Journal Article] Scx+/Sox9+ progenitors contribute to the establishment of the junction between tendon/ligament. (2013)
  • Author(s): Y.Sugimoto,A.Takimoto,H.Akiyama,R.Kist,G.Scherer,T.Nakamura,Y.HIraki,and C.Shukunami
  • Journal Title: Development. Volume: 140 Issue: 11 Pages: 2280-2288
  • DOI: 10.1242/dev.096354
  • Peer Reviewed
• [Journal Article] Tenomodulin expression in the periodontal ligament enhances cellular adhesion (2013)
  • Author(s): Y. Komiyama, S. Ohba, N. Shimohata, K. Nakajima, H. Hojo, F. Yano, T. Takato, D. Docheva, C. Shukunami, Y. Hiraki, and UI. Chung.
  • Journal Title: PLoS One Volume: 8 Issue: 4 Pages: e60203-e60203
  • DOI: 10.1371/journal.pone.0060203
  • Peer Reviewed
• [Journal Article] 腱・靭帯形成の制御メカニズム (2013)
  • Author(s): 宿南知佐
  • Journal Title: Pharma Medica Volume: 31 Pages: 43-46
• [Journal Article] Generation and characterization of ScxCre transgenic mice. (2013)
  • Author(s): Y. Sugimoto, A. Takimoto, Y. Hiraki, C. Shukunami
  • Journal Title: Genesis. Volume: 51 Issue: 4 Pages: 275-283
  • DOI: 10.1002/dvg.22372
  • Peer Reviewed
• [Journal Article] 腱・靱帯形成の制御メカニズム (2013)
  • Author(s): 宿南 知佐
  • Journal Title: Pharma Medica Volume: 31 Pages: 43-46
• [Journal Article] Direct conversion of tenocytes into chondrocytes by Sox9 (2012)
  • Author(s): A. Takimoto, M. Oro, Y. Hiraki, and C. Shukunami
  • Journal Title: Exp Cell Res. Volume: 318 Pages: 1492-1507
  • Peer Reviewed
• [Journal Article] Direct conversion of teonocytes into chondrocytes by Sox9 (2012)
  • Author(s): Takimoto A, 他
  • Journal Title: Exp Cell Res Volume: (in press) Issue: 13 Pages: 1492-1507
  • DOI: 10.1016/j.yexcr.2012.04.002
  • Peer Reviewed
• [Journal Article] 腱の形成と再生-Tenomodulin (2011)
  • Author(s): 宿南知佐
  • Journal Title: 臨床整形外科 Volume: 46 Pages: 528-532
• [Journal Article] Conversion of human bone marrow-derived mesenchymal stem cells into tendon progenitor cells by ectopic expression of scleraxis. (2011)
  • Author(s): P. Alberton, C. Popov, M. Pragert, J. Kohler, C. Shukunami, M. Schieker, and D. Docheva.
  • Journal Title: Stem Cells Dev Volume: 21 Issue: 6 Pages: 846-858
  • DOI: 10.1089/scd.2011.0150
  • Peer Reviewed
• [Journal Article] Yasui Differential expression of Tenomodulin and Chondromodulin-1 at the insertion site of the tendon reflects a phenotypic transition of the resident cells (2010)
  • Author(s): K. Yukata, Y. Matsui, C. Shukunami, A. Takimoto, N. Hirohashi, O. Ohtani, T. Kimura, Y. Hiraki, N
  • Journal Title: Tissue & Cell Volume: 42 Issue: 2 Pages: 116-120
  • DOI: 10.1016/j.tice.2010.02.002
  • Peer Reviewed
• [Journal Article] Differential expression of tenomodulin and chondromodulin-1 at the insertion site of the tendon reflects a phenotypic transtion of the resident cells. (2010)
  • Author(s): Yukata K, 他
  • Journal Title: 臨床整形外科 Volume: 42 Pages: 116-120
  • Peer Reviewed
• [Presentation] 腱・靭帯付着部形成におけるScx/Sox9陽性細胞の役割 (2012)
  • Author(s): 杉本由紀、滝本晶、秋山治彦、中村孝志、開祐司、宿南知佐
  • Organizer: 第15回骨・再生研究会(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-11-10
• [Presentation] 矯正的歯の移動モデルを用いた歯根膜におけるScleraxisの機能解析 (2012)
  • Author(s): 川津正慶、宿南知佐、滝本晶、岩崎将也、清流正弘、池田悦子、山本照子
  • Organizer: 第17回日本矯正歯科学会
  • Place of Presentation: 盛岡
  • Year and Date: 2012-09-27
• [Presentation] 腱・靭帯形成とその制御第 (2012)
  • Author(s): 宿南知佐
  • Organizer: 第30回日本骨代謝学会学術集会(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-07-21
• [Presentation] Scleraxisの発現とその制御 (2012)
  • Author(s): 滝本晶、宿南知佐、川津正慶、清流正弘、山本照子、開祐司
  • Organizer: 第30回日本骨代謝学会学術集会(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-07-19
• [Presentation] 腱・靭帯の形成と再生 (2011)
  • Author(s): 宿南知佐
  • Organizer: 第26回に本整形外科学基礎学術集会(招待講演)
  • Place of Presentation: 群馬県民会館、群馬
  • Year and Date: 2011-10-21
• [Presentation] 腱・靭帯の形成と再生 (2011)
  • Author(s): 宿南知佐
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋(群馬県民会館)(招待講演)
  • Year and Date: 2011-10-21
• [Presentation] 腱・靱帯形成の制御 (2011)
  • Author(s): 宿南知佐
  • Organizer: 第18回プロテオグライカンフォーラム
  • Place of Presentation: 東京医科歯科大学(招待)
  • Year and Date: 2011-02-19
• [Presentation] 腱・靭帯形成の制御 (2011)
  • Author(s): 宿南知佐
  • Organizer: 第18回プロテオグリカンフォーラム(招待講演)
  • Place of Presentation: 東京医科歯科大学、東京
  • Year and Date: 2011-02-09
• [Presentation] Scleraxisの発現とその制御
  • Author(s): 滝本晶、宿南知佐、川津正慶、清流正広、山本照子、開祐司
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 腱・靱帯形成とその制御
  • Author(s): 宿南知佐
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 矯正的歯の移動モデルを用いた歯根膜におけるScleraxisの機能解析
  • Author(s): 川津正慶、宿南知佐、滝本晶、岩崎将也、清流正広、池田悦子、山本照子
  • Organizer: 第71回日本矯正歯科学会
  • Place of Presentation: 盛岡
• [Presentation] 腱・靭帯付着部形成におけるScx/Sox9陽性細胞の役割
  • Author(s): 杉本由紀、滝本晶、秋山治彦、中村孝志、開祐司、宿南知佐
  • Organizer: 第15回骨発生・再生研究会
  • Place of Presentation: 東京
  • Invited
• [Remarks] 研究室のホームページ
  • URL: http://www.frontier.kyoto-u.ac.jp/te01/index-j.htm
• [Remarks] 発表論文(8)に関連して、Stem Cells & Regeneration from Development のImage Galleryに取り上げられた画像が納められているホームページ
  • URL: http://stemcells.dev.biologists.org/page/image-gallery