• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation system of differentiation from mesenchymal stem cells to bone, vascular, and fatty tissues by mechanical loading and unloading

Research Project

Project/Area Number 22390295
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

SAKAI Akinori  産業医科大学, 医学部, 教授 (90248576)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords荷重 / 非荷重 / 骨芽細胞 / 血管内皮細胞 / 脂肪細胞 / 未分化間葉系幹細胞 / 骨髄細胞 / 副甲状腺ホルモン受容体 / 海綿骨 / 皮質骨 / p21 / PTH/PTHrP受容体 / アルデヒド脱水素酵素 / 骨膜 / 副甲状腺ホルモン / CFU-f / オイルレッドO / 骨形成 / CD38 / CD31 / 間葉系幹細胞 / wnt / PECAM-1
Research Abstract

We performed studies using exercise loading mice and unloading mice due to tail suspension. We demonstrated that the signal through PTH/PTHrP receptors promoted osteogenic differentiation and suppressed adipogenic differentiation from mesenchyman stem cells under loading condition. This signal is necessary for promotion of periosteal bone formation and enlargement of cross-sectional cortical shell. The expressions of CD31 and its receptor CD38 decreased after unloading and recovered to the normal loading levels after reloading. Co-culture experiments of osteoblasts and CD38 positive cells revealed that vascular endothelial factors are necessary for osteogenic differentiation.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (57 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (24 results) (of which Peer Reviewed: 12 results) Presentation (22 results) (of which Invited: 5 results) Book (11 results)

  • [Journal Article] 骨粗鬆症患者において他のビスホスホネート製剤からミノドロン酸水和物月1回製剤へ変更後の腰椎骨密度増加に関連する背景因子の検討2014

    • Author(s)
      酒井昭典、ほか
    • Journal Title

      Osteoporosis Japan

      Volume: 22 Pages: 102-103

    • Related Report
      2013 Annual Research Report
  • [Journal Article] A comparative study of the effects of daily minodronate and weekly alendronate on upper gastrointestinal symptoms, bone resorption, and back pain in postmenopausal osteoporosis patients.2013

    • Author(s)
      Yoshioka T, Sakai A, et al.
    • Journal Title

      J Bone Miner Metab

      Volume: 31 Issue: 2 Pages: 153-160

    • DOI

      10.1007/s00774-012-0393-x

    • NAID

      10031168850

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Why not use your own body weight to prevent falls? A randomized, controlled trial of balance therapy to prevent falls and fractures forelderly people who can stand on one leg for <15 s.2013

    • Author(s)
      Sakamoto K, Sakai A, et al.
    • Journal Title

      J Orthop Sci

      Volume: 18 Issue: 1 Pages: 110-120

    • DOI

      10.1007/s00776-012-0328-3

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Disruption of aldehyde dehydrogenase 2 gene results in altered cortical bone structure and ibcreased cortical bone mineral density in the femoral diaphysis of mice.2013

    • Author(s)
      Tsuchiya T, Nakamura T, et al.
    • Journal Title

      Bone

      Volume: 53 Issue: 2 Pages: 358-368

    • DOI

      10.1016/j.bone.2012.12.049

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of human mesenchymal stem cells derived from shoulder tissues involved in rotator cuff tears.2013

    • Author(s)
      Utsunomiya H, Nakamura T, et al.
    • Journal Title

      Am J Sports Med

      Volume: 41 Issue: 3 Pages: 657-668

    • DOI

      10.1177/0363546512473269

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メカニカルストレスとWntシグナル2013

    • Author(s)
      酒井昭典、ほか
    • Journal Title

      Clinical Calcium

      Volume: 23 Pages: 53-59

    • Related Report
      2013 Annual Research Report
  • [Journal Article] ビスホスホネート製剤の現況2013

    • Author(s)
      酒井昭典、ほか
    • Journal Title

      医薬ジャーナル

      Volume: 49 Pages: 873-878

    • Related Report
      2013 Annual Research Report
  • [Journal Article] テリパラチド2013

    • Author(s)
      酒井昭典
    • Journal Title

      Rheumatology Clinical Research

      Volume: 2 Pages: 59-62

    • Related Report
      2013 Annual Research Report
  • [Journal Article] ビスホスホネート月1回製剤をめぐって2013

    • Author(s)
      酒井昭典
    • Journal Title

      Clinic Magazine

      Volume: 531 Pages: 20-22

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 骨粗鬆症の骨量減少防止とカルシトニン -不動性骨粗鬆症、骨粗鬆症性骨折-2013

    • Author(s)
      酒井昭典
    • Journal Title

      医薬ジャーナル

      Volume: 49 Pages: 169-174

    • Related Report
      2013 Annual Research Report
  • [Journal Article] メカニカルストレスの骨細胞による感知とWnt シグナル2012

    • Author(s)
      酒井昭典
    • Journal Title

      Clin Calcium

      Volume: 22(DOI: CliCa121218291835) Pages: 1829-1835

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 抗スクレロスチン抗体の骨形成促進作用と臨床への期待2012

    • Author(s)
      酒井昭典
    • Journal Title

      整形・災害外科

      Volume: 55 Pages: 185-189

    • Related Report
      2012 Annual Research Report
  • [Journal Article] テリパラチド治療における骨吸収抑制剤の役割2012

    • Author(s)
      酒井昭典
    • Journal Title

      MEDICINAL

      Volume: 2 Pages: 109-113

    • Related Report
      2012 Annual Research Report
  • [Journal Article] PTH製剤(特集:骨粗鬆症治療薬のupdate)2012

    • Author(s)
      酒井昭典
    • Journal Title

      骨粗鬆症治療

      Volume: 11 Pages: 184-189

    • NAID

      40019452728

    • Related Report
      2012 Annual Research Report
  • [Journal Article] メカニカルストレスの骨細胞による感知とWntシグナル2012

    • Author(s)
      酒井昭典
    • Journal Title

      Clin Calcium

      Volume: 22 Pages: 1829-1835

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Reduced bone formation in alcohol-induced osteopenia is associated with elevated p21 expression in bone marrow cells in aldehyde dehydrogenase 2-disrupted mice2011

    • Author(s)
      Shimizu Y, et al
    • Journal Title

      Bone

      Volume: 48 Issue: 5 Pages: 1075-1086

    • DOI

      10.1016/j.bone.2011.01.008

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 不動・非荷重モデル2011

    • Author(s)
      酒井昭典
    • Journal Title

      Clinical Calcium

      Volume: 21 Pages: 181-188

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 骨組織に対する荷重の影響2011

    • Author(s)
      酒井昭典
    • Journal Title

      Clinical Calcium

      Volume: 21 Pages: 569-574

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Unipedal standing exercise and hip bone mineral density in postmenopausal women : a randomized controlled trial2010

    • Author(s)
      Sakai A, et al
    • Journal Title

      J Bone Miner Metab

      Volume: 28 Issue: 1 Pages: 42-48

    • DOI

      10.1007/s00774-009-0100-8

    • NAID

      10027206561

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shorter unipedal standing time and lower bone mineral density in women with distal radius fractures2010

    • Author(s)
      Sakai A, et al
    • Journal Title

      Osteoporos Int

      Volume: 21 Issue: 5 Pages: 733-739

    • DOI

      10.1007/s00198-009-0992-0

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of vitamin A delays bone healing process in association with reduced BMP2 expression after drill-hole injury in mice2010

    • Author(s)
      Tanaka K, Sakai A, et al
    • Journal Title

      Bone

      Volume: 47 Issue: 6 Pages: 11006-1012

    • DOI

      10.1016/j.bone.2010.08.016

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unipedal standing exercise and hip bone mineral density in postmenopausal women : arandomized controlled trial.2010

    • Author(s)
      Sakai A, et al.
    • Journal Title

      J Bone Miner Metab

      Volume: 28 Pages: 42-48

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shorter unipedal standing time and lower bone mineral density in women with distal radius fractures.2010

    • Author(s)
      Sakai A, et al
    • Journal Title

      Osteoporos Int

      Volume: 21 Pages: 733-739

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of vitamin A delays bone healing process in association with reduced BMP2 expression after drill-hole injury in mice.2010

    • Author(s)
      Tanaka K, Sakai A, et al.
    • Journal Title

      Bone

      Volume: 47 Pages: 1006-1012

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨粗鬆症患者において他のビスホスホネート製剤からミノドロン酸月1回製剤へ変更後の腰椎骨密度増加に関連する背景因子の検討2013

    • Author(s)
      酒井昭典, 他
    • Organizer
      第15回日本骨粗鬆症学会
    • Place of Presentation
      大阪市(大阪国際会議場)
    • Year and Date
      2013-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 橈骨遠位端骨折の臨床からみた骨粗鬆症治療の問題点と課題2013

    • Author(s)
      酒井昭典
    • Organizer
      第15回日本骨粗鬆症学会
    • Place of Presentation
      大阪市(大阪国際会議場)
    • Year and Date
      2013-10-11
    • Related Report
      2013 Final Research Report
  • [Presentation] トモシンセシス骨梁解析による海綿骨微細構造の推定-イヌ大腿骨および腰椎での検討-2013

    • Author(s)
      山本淳也, 酒井昭典, 他
    • Organizer
      第33回日本骨形態計測学会
    • Place of Presentation
      浜松市(アクトシティ浜松コングレスセンター)
    • Year and Date
      2013-07-06
    • Related Report
      2013 Final Research Report
  • [Presentation] メカニカルストレスと骨髄細胞分化2013

    • Author(s)
      酒井昭典, 他
    • Organizer
      第33回日本骨形態計測学会
    • Place of Presentation
      浜松市(アクトシティ浜松コングレスセンター
    • Year and Date
      2013-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨粗鬆症の動物モデル2012

    • Author(s)
      酒井昭典
    • Organizer
      第27回日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋市(名古屋国際会議場)
    • Year and Date
      2012-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨への力学的負荷の感知とその応答シグナル2012

    • Author(s)
      酒井昭典
    • Organizer
      第30回日本骨代謝学会学術集会
    • Place of Presentation
      東京都(京王プラザホテル)
    • Year and Date
      2012-10-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨芽細胞分化と骨形成の分子制御の最新知見2012

    • Author(s)
      酒井昭典
    • Organizer
      第27回日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋市(名古屋国際会議場)
    • Year and Date
      2012-10-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 卵巣摘出動物におけるPTH のアナボリック作用2012

    • Author(s)
      酒井昭典, 他
    • Organizer
      第32回日本骨形態計測学会
    • Place of Presentation
      大阪市(大阪国際会議場)
    • Year and Date
      2012-06-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨粗鬆症治療の新時代-新規テリパラチド製剤週1回投与法と骨形成促進作用-2012

    • Author(s)
      酒井昭典
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      京都市(国立京都国際会館)
    • Year and Date
      2012-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Fragility fracture : Risk assessment and prevention2011

    • Author(s)
      Sakai A, et al
    • Organizer
      第13回日本骨粗鬆症学会 3rd Joint Meeting of Japanese Osteoporosis Society and Korean Society of Osteoporosis Symposium
    • Place of Presentation
      神戸市(神戸国際会議場)
    • Year and Date
      2011-11-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Fragility fracture : Risk assessment and prevention2011

    • Author(s)
      Sakai A, et al
    • Organizer
      第13回日本骨粗鬆症学会3rd Joint Meeting of Japanese Osteoporosis Society and Korean Society of Osteoporosis Symposium
    • Place of Presentation
      神戸(神戸国際会議場)(招待講演)
    • Year and Date
      2011-11-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 非荷重環境下でのTie-2 受容体の発現と局在の変化2010

    • Author(s)
      森寺邦晃, 酒井昭典, 他
    • Organizer
      第28回日本骨代謝学会学術集会
    • Place of Presentation
      東京都(京王プラザホテル)
    • Year and Date
      2010-07-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 非荷重環境下でのTie-2受容体の発現と局在の変化2010

    • Author(s)
      森寺邦晃、酒井昭典, ほか
    • Organizer
      第28回日本骨代謝学会学術集会
    • Place of Presentation
      東京(京王プラザホテル)
    • Year and Date
      2010-07-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] メカニカルストレスと骨髄細胞分化

    • Author(s)
      酒井昭典、ほか
    • Organizer
      第33回日本骨形態計測学会
    • Place of Presentation
      浜松市(アクトシティ浜松コングレスセンター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] トモシンセシス骨梁解析による海綿骨微細構造の推定 -イヌ大腿骨および腰椎での検討-

    • Author(s)
      山本淳也、酒井昭典
    • Organizer
      第33回日本骨形態計測学会
    • Place of Presentation
      浜松市(アクトシティ浜松コングレスセンター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 橈骨遠位端骨折の臨床からみた骨粗鬆症治療の問題点と課題

    • Author(s)
      酒井昭典
    • Organizer
      第15回日本骨粗鬆症学会
    • Place of Presentation
      大阪市(大阪国際会議場)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨粗鬆症患者において他のビスホスホネート製剤からミノドロン酸月1回製剤へ変更後の腰椎骨密度増加に関連する背景因子の検討

    • Author(s)
      酒井昭典、ほか
    • Organizer
      第15回日本骨粗鬆症学会
    • Place of Presentation
      大阪市(大阪国際会議場)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 卵巣摘出動物におけるPTHのアナボリック作用

    • Author(s)
      酒井昭典
    • Organizer
      第32回 日本骨形態計測学会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 骨への力学的負荷の感知とその応答シグナル

    • Author(s)
      酒井昭典
    • Organizer
      第30回 日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 非定型骨折の疫学、海外での現状

    • Author(s)
      酒井昭典
    • Organizer
      第14回 日本骨粗鬆症学会
    • Place of Presentation
      新潟コンベンションセンター(新潟市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 骨芽細胞分化と骨形成の分子制御の最新知見

    • Author(s)
      酒井昭典
    • Organizer
      第27回 日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 骨粗鬆症の動物モデル

    • Author(s)
      酒井昭典
    • Organizer
      第27回 日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] ファーマナビゲーターPTH 編2013

    • Author(s)
      酒井昭典
    • Total Pages
      317
    • Publisher
      メディカルレビュー社
    • Related Report
      2013 Final Research Report
  • [Book] ファーマナビゲータービスホスホネート編2013

    • Author(s)
      酒井昭典
    • Total Pages
      249
    • Publisher
      メディカルレビュー社
    • Related Report
      2013 Final Research Report
  • [Book] ビスホスホネートエビデンスブック2013

    • Author(s)
      酒井昭典
    • Total Pages
      383
    • Publisher
      医薬ジャーナル社
    • Related Report
      2013 Final Research Report
  • [Book] ファーマナビゲーター PTH編2013

    • Author(s)
      酒井昭典
    • Total Pages
      317
    • Publisher
      メディカルレビュー社
    • Related Report
      2013 Annual Research Report
  • [Book] ビスホスホネート エビデンスブック2013

    • Author(s)
      酒井昭典
    • Total Pages
      383
    • Publisher
      医薬ジャーナル社
    • Related Report
      2013 Annual Research Report
  • [Book] ファーマナビゲーター活性型ビタミンD3製剤編2012

    • Author(s)
      酒井昭典
    • Total Pages
      355
    • Publisher
      メディカルレビュー社
    • Related Report
      2013 Final Research Report
  • [Book] 骨粗鬆症診療ハンドブック(改訂5版)2012

    • Author(s)
      酒井昭典
    • Total Pages
      391
    • Publisher
      医薬ジャーナル社
    • Related Report
      2013 Final Research Report
  • [Book] 骨粗鬆症診療ハンドブック 改訂5版2012

    • Author(s)
      酒井昭典
    • Total Pages
      391
    • Publisher
      ビスホスホネート
    • Related Report
      2012 Annual Research Report
  • [Book] ファーマナビゲーター 活性型ビタミンD3 製剤編2012

    • Author(s)
      酒井昭典
    • Total Pages
      355
    • Publisher
      不動性骨粗鬆症
    • Related Report
      2012 Annual Research Report
  • [Book] Mechanosensing Biology2011

    • Author(s)
      Sakai A, Nakamura T
    • Total Pages
      219
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] 整形外科専門医テキスト2010

    • Author(s)
      酒井昭典, 中村利孝
    • Total Pages
      987
    • Publisher
      南江堂
    • Related Report
      2013 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi