Regulation system of differentiation from mesenchymal stem cells to bone, vascular, and fatty tissues by mechanical loading and unloading
| Project/Area Number |
22390295
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
SAKAI Akinori 産業医科大学, 医学部, 教授 (90248576)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 荷重 / 非荷重 / 骨芽細胞 / 血管内皮細胞 / 脂肪細胞 / 未分化間葉系幹細胞 / 骨髄細胞 / 副甲状腺ホルモン受容体 / 海綿骨 / 皮質骨 / p21 / PTH/PTHrP受容体 / アルデヒド脱水素酵素 / 骨膜 / 副甲状腺ホルモン / CFU-f / オイルレッドO / 骨形成 / CD38 / CD31 / 間葉系幹細胞 / wnt / PECAM-1 |
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| Research Abstract |
We performed studies using exercise loading mice and unloading mice due to tail suspension. We demonstrated that the signal through PTH/PTHrP receptors promoted osteogenic differentiation and suppressed adipogenic differentiation from mesenchyman stem cells under loading condition. This signal is necessary for promotion of periosteal bone formation and enlargement of cross-sectional cortical shell. The expressions of CD31 and its receptor CD38 decreased after unloading and recovered to the normal loading levels after reloading. Co-culture experiments of osteoblasts and CD38 positive cells revealed that vascular endothelial factors are necessary for osteogenic differentiation.
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Report
(5 results)
Research Products
(57 results)
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[Presentation] 骨粗鬆症の動物モデル2012
Author(s)
酒井昭典
Organizer
第27回日本整形外科学会基礎学術集会
Place of Presentation
名古屋市(名古屋国際会議場)
Year and Date
2012-10-27
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[Presentation] 骨粗鬆症の動物モデル
Author(s)
酒井昭典
Organizer
第27回 日本整形外科学会基礎学術集会
Place of Presentation
名古屋国際会議場（名古屋市）
Related Report
Invited
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