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Investigation of molecular machamism of periopearetive oxygen metabolism regulation by hypoxia-inducible factors

Research Project

Project/Area Number 22390298
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKyoto University

Principal Investigator

HIROTA Kiichi  京都大学, 医学研究科, 准教授 (00283606)

Co-Investigator(Kenkyū-buntansha) HOSHINO Yuma  京都大学, 医学研究科, 特任講師 (00378746)
KONDOH Shinae  東京工業大学, 生命理工学研究科, 教授 (40314182)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords薬理学 / 麻酔薬 / 低酸素応答 / 細胞生物学 / 分子生物学 / 低酸素 / HIF-1 / 周術期 / 麻酔科学 / 低酸素誘導性因子1 / 酸素代謝 / 低酸素誘導性因子
Research Abstract

Oxygen is one of the most important vital molecules for almost all higher organism including human beings. In contrast, excessive oxygen is cytotoxic. A bottom line of the perioperative patient care is to maintain oxygen supply/demand within a narrow optimized range. Hypoxia-inducible factors (HIFs) play a central role in regulation of oxygen homeostasis. To investigate the dynamism of perioperative oxygen metabolism from the point of view of HIFs, we have established the experimental systems adopting cultured cells and experimental animals and obtained several experimental results.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 11 results) Presentation (24 results) (of which Invited: 4 results) Book (2 results)

  • [Journal Article] Involvement of decreased hypoxia-inducible factor 1 activity and resultant G(1)-S cell cycle transition in radioresistance of perinecrotic tumor cells2013

    • Author(s)
      Y. Zhu, T. Zhao, S. Itasaka, L. Zeng, C.J. Yeom, K. Hirota, K. Suzuki, A. Morinibu, K. Shinomiya, G. Ou, M. Yoshimura, M. Hiraoka, H. Harada
    • Journal Title

      Oncogene

      Volume: 32 Issue: 16 Pages: 2058-2068

    • DOI

      10.1038/onc.2012.223

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of gankyrin in mouse hepatocytes induces hemangioma by suppressing factor inhibiting hypoxia-inducible factor-1 (FIH-1) and activating hypoxia-inducible factor-12013

    • Author(s)
      Y. Liu, H. Higashitsuji, K. Itoh, T. Sakurai, K. Koike, K. Hirota, M. Fukumoto, J. Fujita
    • Journal Title

      Biochemical and biophysical research communications

      Volume: 432 Issue: 1 Pages: 22-27

    • DOI

      10.1016/j.bbrc.2013.01.093

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of n-propyl gallate on neuronal survival after forebrain ischemia in rats2012

    • Author(s)
      Y. Kawano, M. Kawaguchi, K. Hirota, S. Kai, N. Konishi, H. Furuya
    • Journal Title

      Resuscitation

      Volume: 83 Issue: 2 Pages: 249-252

    • DOI

      10.1016/j.resuscitation.2011.07.024

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cancer cells that survive radiation therapy acquire HIF-1 activity and translocate towards tumour blood vessels2012

    • Author(s)
      H. Harada, M. Inoue, S. Itasaka, K. Hirota,A. Morinibu, K. Shinomiya, L. Zeng, G. Ou, Y. Zhu, M. Yoshimura, W.G. McKenna, R.J. Muschel, M. Hiraoka
    • Journal Title

      Nature communications

      Volume: 3 Issue: 1 Pages: 783-783

    • DOI

      10.1038/ncomms1786

    • NAID

      120004007582

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypoxia and hypoxia-inducible factor in inflammation2012

    • Author(s)
      Kiichi, Hirota
    • Journal Title

      Chronic Inflammation: Molecular Pathophysiology, Nutritional and Therapeutic Interventions

      Volume: 1 Pages: 51-65

    • Related Report
      2012 Annual Research Report
  • [Journal Article] グルタミノリシス2012

    • Author(s)
      広田喜一
    • Journal Title

      実験医学

      Volume: 30 Pages: 2400-2406

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Hydrogen sulfide inhibits hypoxia- but not anoxia-induced hypoxia-inducible factor 1 activation in a von hippel-lindau- and mitochondria-dependent manner.2011

    • Author(s)
      甲斐慎一、田中具治、大条紘樹、原田浩、岸本俊、鈴木堅悟、高淵聡史、竹永敬三、福田和彦、広田喜一
    • Journal Title

      Antioxid Redox Signal

      Volume: 16 Issue: 3 Pages: 203-216

    • DOI

      10.1089/ars.2011.3882

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 炎症の制御点としての低酸素誘導性因子2011

    • Author(s)
      広田 喜一, 大条 鉱樹, 甲斐 慎一, 鈴木堅悟, 岸本 俊, 松山 智紀
    • Journal Title

      麻酔

      Volume: 60

    • Related Report
      2012 Final Research Report
  • [Journal Article] 周術期医学としてのハイポキシア生物学の探究2011

    • Author(s)
      広田 喜一
    • Journal Title

      麻酔

      Volume: 60

    • Related Report
      2012 Final Research Report
  • [Journal Article] General anesthetics inhibit erythropoietin induction under hypoxic conditions in the mouse brain.2011

    • Author(s)
      田中具治、甲斐慎一、小山智弘、大条紘樹、足立健彦、福田和彦、広田喜一
    • Journal Title

      PLoS One

      Volume: 6 Issue: 12 Pages: 29378-29378

    • DOI

      10.1371/journal.pone.0029378

    • NAID

      120003726125

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of the onset of ischemia and carcinogenesis by hypoxia-inducible transcription factor-based in vivo bioluminescence imaging2011

    • Author(s)
      Kadonosono T, Kuchimaru T, Yamada S, Takahashi Y. Murakami A, Watanabe H, Tani T, Inoue M, Tsukamoto T, Toyoda T, Tanaka T, Hirota K, Urano K, Machida K, Eto T, Ogura T, Tsutsumi H, Ito M, Hiraoka M, Kondoh G, Kizaka-Kondoh S
    • Journal Title

      PLoS ONE

      Volume: 6(11) Issue: 11 Pages: e26640-e26640

    • DOI

      10.1371/journal.pone.0026640

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fentanyl activates hypoxia-inducible factor 1 in neuronal SH-SY5Y cells and mice under non-hypoxic conditions in a μ-opioid receptor-dependent manner.2011

    • Author(s)
      大条紘樹、甲斐慎一、田中具治、若松拓彦、岸本俊、鈴木堅悟、原田浩、高淵聡史、足立健彦、福田和彦、広田喜一
    • Journal Title

      Eur J Pharmacol

      Volume: 667 Issue: 1-3 Pages: 144-152

    • DOI

      10.1016/j.ejphar.2011.06.014

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 炎症の制御点としての低酸素誘導性因子2011

    • Author(s)
      広田喜一, 他
    • Journal Title

      麻酔

      Volume: 60

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 周術期医学としてのハイポキシア生物学の探究2011

    • Author(s)
      広田喜一
    • Journal Title

      麻酔

      Volume: 60

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Persisting mild hypothermia suppresses hypoxia-inducible factor-1alpha protein synthesis and hypoxia-inducible factor-1-mediated gene expression.2010

    • Author(s)
      田中具治、若松拓彦、大条紘樹、小田聖子、甲斐慎一、足立健彦、木坂(近藤)科江、福田和彦、広田喜一
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol

      Volume: 298 Issue: 3 Pages: 661-671

    • DOI

      10.1152/ajpregu.00732.2009

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Persisting mild hypothermia suppresses hypoxia-inducible factor 1{alpha} protein synthesis and HIF-1-mediated gene expression.2010

    • Author(s)
      Tanaka, Tomohiro, 他
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol.

      Volume: 293

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The intravenous anesthetic propofol inhibits lipopolysaccharide-induced hypoxia-inducible factor 1 activation and suppresses the glucose metabolism in macrophages.2010

    • Author(s)
      Tanaka, Tomohiro, 他
    • Journal Title

      Journal of Anesthesia

      Volume: 24 Pages: 54-60

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] シンポジウム「低酸素応答が制御する多彩な生命現象」炎症・インフラマソームのレドックス制御とTXNIP/HIF-1システム2012

    • Author(s)
      広田喜一
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡市
    • Year and Date
      2012-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] シンポジウム「低酸素研究の10年」hypoxia-inducible factorの20年と5つの未解決課題2012

    • Author(s)
      広田喜一
    • Organizer
      第10回ガンとハイポキシア研究会
    • Place of Presentation
      横浜市
    • Year and Date
      2012-12-06
    • Related Report
      2012 Final Research Report
  • [Presentation] シンポジウム 「生体ガスと臓器保護」硫化水素はミトコンドリア依存的に低酸素誘導性遺伝子応答を調節する2012

    • Author(s)
      広田喜一、甲斐慎一
    • Organizer
      第16回医療ガス学会学術大会
    • Place of Presentation
      東京都
    • Year and Date
      2012-11-17
    • Related Report
      2012 Final Research Report
  • [Presentation] 特別講演 ハイポキシア生物学ー酸素代謝から見る生命現象の方程式2012

    • Author(s)
      広田喜一
    • Organizer
      第47回日本高気圧環境・潜水医学会学術総会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-11-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Possible Involvement of Hypoxia-Inducible Factor 1 in Activation of Microglia and Subsequent Gene Expression Conferring Neuropathic Pain.2012

    • Author(s)
      Tomonori Matsuyama, Kiichi Hirota, Kengo Suzuki, Shinichi Kai, Kazuhiko Fukuda
    • Organizer
      2012 Annual Meeting, American Society of Anesthesiologists
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2012-10-15
    • Related Report
      2012 Final Research Report
  • [Presentation] イソフルランは、急性貧血により誘導される血中エリスロポエチンの上昇を抑制する2012

    • Author(s)
      甲斐慎一、田中具治、松山智紀、大条紘樹、広田喜一、福田和彦
    • Organizer
      日本麻酔科学会 第59回学術総会
    • Place of Presentation
      福岡市
    • Year and Date
      2012-06-07
    • Related Report
      2012 Final Research Report
  • [Presentation] マイクログリアにおける活性化刺激の低酸素応答に及ぼす影響の検討日本麻酔科学会2012

    • Author(s)
      松山智紀, 広田喜一, 鈴 堅悟, 甲斐慎一, 大条紘樹, 福田和彦
    • Organizer
      第59回学術総会
    • Place of Presentation
      福岡市
    • Year and Date
      2012-06-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 炎症の制御点としての低酸素誘導性因子2012

    • Author(s)
      広田喜一, 他
    • Organizer
      日本麻酔科学会第58回学術集会
    • Place of Presentation
      神戸市(神戸ポートピアホテル・神戸国際会議場)
    • Year and Date
      2012-05-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 周術期医学としてのハイポキシア生物学の探究2012

    • Author(s)
      広田喜一
    • Organizer
      日本麻酔科学会第58回学術集会
    • Place of Presentation
      神戸市(神戸ポートピアホテル・神戸国際会議場)
    • Year and Date
      2012-05-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 炎症・インフラマソームのレドックス制御とTXNIP/HIF-1システム2012

    • Author(s)
      広田喜一
    • Organizer
      第85回日本生化学会 シンポジウム:1S12 低酸素応答が制御する多彩な生命現象 -生理機能の解明から疾患に迫る
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] TXNIP regulates the NLPR3 inflammatosome activity in a HIF-1- and thioredoxin-dependent manner in THP-1 macrophages2012

    • Author(s)
      Kiichi Hirota
    • Organizer
      33rd Naito Conference Oxygen Biology Hypoxia, Odidative stress and Diseases
    • Place of Presentation
      札幌市
    • Related Report
      2012 Annual Research Report
  • [Presentation] hypoxia-indicible factorの20年と5つの未解決問題2012

    • Author(s)
      広田喜一
    • Organizer
      第10回がんとハイポキシア研究会
    • Place of Presentation
      横浜市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ハイポキシア生物学ー酸素代謝からみる生命現象の方程式2012

    • Author(s)
      広田喜一
    • Organizer
      第47回日本高気圧環境・潜水医学会学術集会
    • Place of Presentation
      札幌市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Isoflurane selectively inhibits the activation of hypoxia-inducible factor 2 and the induction of erythropoietin under hypoxic conditions in mouse brain 2011 Annual Meeting2011

    • Author(s)
      Kiichi Hirota, Tomoharu Tanaka, Shinichi Kai, Tomohiro Koyama, Hiroki Daijo, Kengo Suzuki, Takehiko Adachi, Kazuhiko Fukuda
    • Organizer
      American Society of Anesthesiologists
    • Place of Presentation
      Chicago USA
    • Year and Date
      2011-10-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 炎症の制御点としての低酸素誘導性因子2011

    • Author(s)
      広田喜一,大条紘樹,甲斐慎一,鈴木堅悟,岸本俊, 松山智紀
    • Organizer
      日本麻酔科学会 第58回学術集会
    • Place of Presentation
      神戸市
    • Year and Date
      2011-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] イソフルランは低酸素によるエリスロポエチンの上昇を抑制する2011

    • Author(s)
      甲斐慎一,田中具治、鈴木堅悟,大条鉱樹、広田喜一、福田和彦
    • Organizer
      日本麻酔科学会 第58回学術総会
    • Place of Presentation
      神戸市
    • Year and Date
      2011-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] プロスタグランディンE1はHIF-1活性化、 VEGF発現を介して血管透過性を亢進させる2011

    • Author(s)
      鈴木堅悟、広田喜一、大条鉱樹、甲斐慎一、岸本俊、福田和彦
    • Organizer
      日本麻酔科学会 第58回学術総会
    • Place of Presentation
      神戸市
    • Year and Date
      2011-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 学会賞記念講演 周術期医学としてのハイポキシア生物学の探求2011

    • Author(s)
      広田 喜一
    • Organizer
      第58回日本麻酔科学会学術集会
    • Place of Presentation
      神戸市
    • Year and Date
      2011-05-19
    • Related Report
      2012 Final Research Report
  • [Presentation] タバコ煙抽出物はヒト肺胞II型上皮細胞株(A549)において低酸素誘導性因子1(hypoxia-inducible factor 1:HIF-1)を活性化する2011

    • Author(s)
      大条紘樹、鈴木堅固,岸本俊、甲斐慎一、広田喜一、福田和彦
    • Organizer
      日本麻酔科学会 第58回学術総会
    • Place of Presentation
      神戸市
    • Year and Date
      2011-05-19
    • Related Report
      2012 Final Research Report
  • [Presentation] タバコ煙抽出物は低酸素誘導性因子1(hypoxia-inducible factor 1 : HIF-1)を活性化する2011

    • Author(s)
      大条鉱樹, 他
    • Organizer
      第8回がんとハイポキシア研究会
    • Place of Presentation
      北海道大学学術交流会館,札幌市
    • Year and Date
      2011-01-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 硫化水素が低酸素誘導性HIF-1活性化に及ぼす影響2011

    • Author(s)
      甲斐慎一, 他
    • Organizer
      第8回がんとハイポキシア研究会
    • Place of Presentation
      北海道大学学術交流会館,札幌市
    • Year and Date
      2011-01-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 硫化水素が低酸素誘導性遺伝子応答に及ぼす影響2010

    • Author(s)
      甲斐 慎一、大条 紘樹、田中 具治、広田喜一、福田 和彦
    • Organizer
      日本麻酔科学会第57回学術総
    • Place of Presentation
      福岡市
    • Year and Date
      2010-06-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 全身麻酔下では、低酸素による脳内エリスロポエチン発現誘導が抑制される2010

    • Author(s)
      田中 具治、広田 喜一、大条 紘樹、甲斐慎一、小山 智弘、福田 和彦
    • Organizer
      日本麻酔科学会第57回学術総会
    • Place of Presentation
      福岡市
    • Year and Date
      2010-06-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 硫化水素はミトコンドリア依存的に低酸素誘導性遺伝子応答を調節する

    • Author(s)
      広田喜一
    • Organizer
      第16回日本医療ガス学会学術集会
    • Place of Presentation
      東京都
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] グルタミノリシス, in: 曽我 朋義, 江角浩安 (編), がんと代謝 (実験医学 増刊)2012

    • Author(s)
      広田 喜一
    • Publisher
      羊土社, 東京
    • Related Report
      2012 Final Research Report
  • [Book] Hypoxia and hypoxia-inducible factor in inflammation, in: S. Roy (Ed.), Chronic Inflammation: Molecular Pathophysiology, Nutritional and Therapeutic Interventions2012

    • Author(s)
      K. Hirota
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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