| Co-Investigator(Kenkyū-buntansha) |
KAMIMURA Noritaka 弘前大学, 医学研究科, 准教授 (00281931)
HABUCHI Tomonori 秋田大学, 医学系研究科, 教授 (00293861)
TSUBOI Shigeru 弘前大学, 医学研究科, 研究員 (20526727)
MORI Kazuyuki 弘前大学, 医学研究科, 助教 (40266903)
KOIE Takuya 弘前大学, 医学部附属病院, 講師 (60321965)
|
|---|
| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
|
|---|
| Outline of Final Research Achievements |
To develop an assay system to measure prostate specific antigen (PSA) with cancer-associated aberrant glycosylation and to compare the diagnostic accuracy with conventional PSA test and %free PSA.We previously identified prostate cancer (PCa)-associated aberrant glycosylation on PSA, that is, α2,3-linked sialylation as an additional terminal N-glycan on free PSA (S2,3PSA). In the present study, we developed a new assay system for measurement of S2,3PSA with magnetic microbead-based immunoassay. We used the magplex beads to measure serum S2,3PSA, employing anti-human free PSA monoclonal antibody (8A6) for coating beads and anti-α2,3-linked sialic acid monoclonal antibody (HYB4) for detection. Area under the curve (AUC) for detection of PCa with S2,3 PSA was 0.84 which was significantly higher than those with PSA (0.57) or %free PSA (0.61). (P<0.001) The novel assay system had significantly higher specificity than conventional PSA test.
|
