Exploration of endometrial cancer-associated microRNAs by using a functional screening method and its application to medication

• Project/Area Number: 22390313
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: AOKI Daisuke 慶應義塾大学, 医学部, 教授 (30167788)
• Co-Investigator(Kenkyū-buntansha): BANNO Kouji 慶應義塾大学, 医学部, 講師 (70265875) ; HIRASAWA Akira 慶應義塾大学, 医学部, 助教 (90296658)
• Co-Investigator(Renkei-kenkyūsha): NOMURA Hiroyukiu 慶應義塾大学, 医学部, 助教 (50327590)
• Research Collaborator: INAZAWA Johji 東京医科歯科大学, 難治疾患研究所, 教授 (30193551) ; KOZAKI Ken-ichi 東京医科歯科大学, 難治疾患研究所, 准教授 (50270715)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 子宮体癌 / マイクロRNA / エピゲノム異常 / DNAメチル化 / 癌抑制型マイクロRNA / 癌 / ゲノム / 核酸 / microRNA / 抗癌剤感受性 / micro RNA / 癌抑制遺伝子 / がん抑制遺伝子

Research Abstract

The aim of this study is to identify a tumor-suppressor microRNA (miRNA) silenced by aberrant DNA methylation in endometrial cancer. By investigating escalation of expression level of microRNAs after exposure of 5-Aza-CdR in endometrial cancer cells, miR-

Research Products

• [Journal Article] MicroRNAs in endometrial cancer. (2013)
  • Author(s): Banno K, Yanokura M, Kisu I, Yamagami W, Susumu N, Aoki D
  • Journal Title: International journal of clinical oncology Volume: 18 Issue: 2 Pages: 186-192
  • DOI: 10.1007/s10147-013-0526-9
  • NAID: 10031176254
  • Peer Reviewed
• [Journal Article] Endometrial Cancer and Hypermethylation: Regulation of DNA and MicroRNA by Epigenetics. (2012)
  • Author(s): Banno K, Kisu I, Yanokura M, Masuda K, Kobayashi Y, Ueki A, Tsuji K, Yamagami W, Nomura H, Susumu N, Aoki D
  • Journal Title: Biochemistry Research International Volume: 2012 Pages: 738274-738274
  • DOI: 10.1155/2012/738274
  • Peer Reviewed
• [Journal Article] Epigenetics and genetics in endometrial cancer: new carcinogenic mechanisms and relationship with clinical practice. (2012)
  • Author(s): Banno K, Kisu I, Yanokura M, Masuda K, Ueki A, Kobayashi Y, Susumu N, Aoki D
  • Journal Title: Epigenomics Volume: 4 Issue: 2 Pages: 147-162
  • DOI: 10.2217/epi.12.13
  • Peer Reviewed
• [Journal Article] Epigenetics and genetics in endometrial cancer : new carcinogenic mechanisms and relationship with clinical practice (2012)
  • Author(s): Banno K, et al
  • Journal Title: Epigenomics Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Endometrial Cancer and Hypermethylation : Regulation of DNA and micro RNA by Epigenetics (2012)
  • Author(s): Banno K, et al
  • Journal Title: Biochemistry Research International Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] MicroRNA in endometrial cancer. (2011)
  • Author(s): Yanokura M, Banno K, Kisu I, Masuda K, Ueki A, Kobayashi Y, Tsuji K, Yamagami W, Nomura H, Hirasawa A, Susumu N, Aoki D
  • Journal Title: Trends Cancer Res Volume: 7 Pages: 49-55
  • NAID: 10031176254
  • URL: http://www.researchtrends.net/tia/title.asp?id=57
  • Peer Reviewed
• [Journal Article] Micro RNA in endometrial cancer (2011)
  • Author(s): Yanokura M, Banno K, et al
  • Journal Title: Trends in Cancer Research Volume: VOL.7 Pages: 49-55
  • NAID: 10031176254
  • Peer Reviewed
• [Presentation] Aberrant epigenetic DNA hypermethylation in liquid-based endometrial cytology samples: evaluation and potential use in diagnosis. (2012)
  • Author(s): Banno K
  • Organizer: 37th Europian congress of Cytology
  • Place of Presentation: ドゥブロヴニク(クロアチア)
  • Year and Date: 2012-10-01
• [Presentation] Antitumor effect of combined miR-34b and paclitaxel on endometrial cancer cells. (2012)
  • Author(s): 矢野倉 恵
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌・札幌市教育文化会館・さっぽろ芸文館(北海道)
  • Year and Date: 2012-09-21
• [Presentation] 子宮体癌においてエピジェネティックに制御されるmiR-34bの同定とその癌抑制効果 (2012)
  • Author(s): 矢野倉 恵
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸ポートピアホテル・神戸国際展示場(兵庫県)
  • Year and Date: 2012-04-13
• [Presentation] Epigenetically controlled miR-34b has antitumor activity against endometrial cancer cells. (2011)
  • Author(s): Banno K
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-04
• [Presentation] エピジェネティックに制御されるmiR-34bは子宮体癌細胞に対して癌抑制効果を示す (2011)
  • Author(s): 阪埜浩司
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-04
• [Presentation] エピゲノム異常により発現抑制され細胞増殖抑制活性を有する子宮体がん関連microRNA及びその標的分子の同定. (2011)
  • Author(s): 鶴田智彦
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: リーガロイヤルホテル大阪・大阪国際会議場(大阪府)
  • Year and Date: 2011-08-30
• [Presentation] エピゲノム異常により発現抑制され細胞増殖抑制活性を有する子宮体がん関連microRNA及びその標的分子の同定 (2011)
  • Author(s): 鶴田智彦
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: リーガロイヤルホテル大阪・大阪国際会議場(大阪府)
  • Year and Date: 2011-08-30
• [Presentation] Functional screening of tumor-suppressive microRNA silenced by DNA hypermethylation in endometrial cancer. (2010)
  • Author(s): Tsuruta T
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル大阪(大阪府)
  • Year and Date: 2010-09-24
• [Presentation] 子宮体癌細胞株の機能的スクリーニングを用いたエピゲノム異常により発現抑制される癌抑制遺伝子型microRNAの同定 (2010)
  • Author(s): 鶴田智彦、小崎健一、平沢晃、阪埜浩司、進伸幸、井本逸勢、青木大輔、稲澤譲治
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] 子宮体癌においてエピジェネテｨックに制御されるmiR-34bの同定とその癌抑制効果
  • Author(s): 矢野倉 恵
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸ポートピアホテル・神戸国際展示場（兵庫県）
• [Presentation] 子宮体癌に対するmiR-34bとパクリタキセル併用による抗腫瘍効果の検討
  • Author(s): 矢野倉 恵
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌 ・札幌市教育文化会館 ・さっぽろ芸文館 （北海道）
• [Presentation] Aberrant epigenetic DNA hypermethylation in liquid-based endometrial cytology samples: evaluation and potential use in diagnosis
  • Author(s): Banno K
  • Organizer: 37th Europian congress of Cytology
  • Place of Presentation: ドゥブロヴニク（クロアチア）
• [Book] Epigenetics in Human Disease (2012)
  • Author(s): Masuda K, Banno K, Yanokura M, Tsuji K, Kisu I, Ueki A, Kobayashi Y, Nomura H, Hirasawa A, Susumu N, Aoki D
  • Publisher: London, UK: Academic Press
• [Book] Epigenetics in Human Disease (2012)
  • Author(s): Masuda K
  • Total Pages: 592
  • Publisher: Academic Press