Study on the survival strategies for periodontal bacteria in host cells and their role in the pathogenesis of periodontal diseases

• Project/Area Number: 22390342
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Okayama University
• Principal Investigator: OHARA Naoya 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70223930)
• Co-Investigator(Kenkyū-buntansha): NAKAYAMA Masaaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10579105) ; OHARA Naoko 岡山大学, 大学院・医歯薬学総合研究科, 助教 (80301365)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 歯周病原細菌 / 細胞内寄生 / Porphyromonas / 嫌気性菌 / 感染 / 歯周病細菌 / gingivalis

Research Abstract

Porphyromonas gingivalisis the major periodontal pathogens. 1) We identified P. gingivalis genes required for survival in the host cells using newly constructed transposon mutagenesis library of P. gingivalis. 2) We found the novel cellular events in host epithelial cells by challenging of P. gingivalis. Akt and its substrates were dephosphorylated by P. gingivalis infection. These results indicated that P. gingivalis-infection may affect cellular functions including apoptosis, glucose metabolism and cell development in host cells by inactivating the PI3K/AKt pathway.

Research Products

• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis. (2011)
  • Author(s): Yukitake, Hideharu
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 141-153
  • Peer Reviewed
• [Journal Article] Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis. (2010)
  • Author(s): Kondo. Yoshio
  • Journal Title: Infection and Immunity Volume: 78 Pages: 2846-2856
  • Peer Reviewed
• [Presentation] P. gingivalisジンジパインによるPI3K/Akt経路の抑制機序 (2013)
  • Author(s): 中山真彰、井上哲圭、大原直也
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] P. gingivalisのECFシグマ因子PG1318 変異株はミューテーター形質を示す (2013)
  • Author(s): 菊池有一郎、柴山和子、国分栄二、大原直也、中山浩次、石原和幸
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] P. gingivalisジンジパインによるPI3K/Akt経路の抑制機序 (2013)
  • Author(s): 中山真彰，井上哲圭，内藤真理子，中山浩次，大原直也
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] Porphyromonas gingivalisによる薬剤排出ポンプ様遺伝子破壊株の薬剤感受性 (2013)
  • Author(s): 井上哲圭，中山真彰，大原直也
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] P. gingivalisのECFシグマ因子PG1318変異株はミューテーター形質を示す (2013)
  • Author(s): 菊池有一郎，柴山和子，国分栄仁，大原直也，中山浩次，石原和幸
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] Porphyromonas gingivali によるAkt/GSK3 beta pathwayの制御 (2012)
  • Author(s): 中山真彰,井上哲圭,大原直也
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山市
  • Year and Date: 2012-09-16
• [Presentation] New aspect of signaling events in Porphyromonas gingivalispathogenesis (2012)
  • Author(s): 大原直也
  • Organizer: First International Conference on Porphyromonas gingivalisand related Bacterial Species
  • Place of Presentation: 長崎市
  • Year and Date: 2012-08-27
• [Presentation] 歯周病原細菌およびその病原性因子の全身疾患への影響 (2012)
  • Author(s): 大原直也
  • Organizer: 第60回日本化学療法学会学術集会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-04-26
• [Presentation] The significance of Akt dephosphorylation of Porphyromonas gingivalis (2012)
  • Author(s): 中山真彰，大原直也
  • Organizer: First International Conference on Porphyromonas gingivalis and related Bacterial Species
  • Place of Presentation: 長崎大学良順会館（長崎市）
• [Presentation] 歯肉上皮細胞におけるPorphyromonas gingivalis HbRによるIL-8産生機序 (2012)
  • Author(s): 大原直也
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] Porphyromonas gingivalisによるAkt脱リン酸化機構とその意義 (2012)
  • Author(s): 中山真彰
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] Inhibition of RANK ligand- induced osteoclast differentiation by Porphyromonas gingivalis. (2011)
  • Author(s): Nakayama M, Nakayama K, Kobayashi K, Ohara N
  • Organizer: XIII International Congress of Bacteriology and Applied MicrobiologyXIII International Congress of Bacteriology and Applied Microbiology, International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Roles of the extracytoplasmic function sigma factors in Porphyromonas gingivalis. (2011)
  • Author(s): Kikuchi Y, Onozawa S, Kiso A, Miyashita M, Ueda O, Hirai K, Shibata Y, Ohara N, Nakayama K, Fujimura S
  • Organizer: XIII International Congress of Bacteriology and Applied MicrobiologyXIII International Congress of Bacteriology and Applied Microbiology, International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Inhibition of RANK ligand-induced osteoclast differentiation by Porphyromonas gingivalis (2011)
  • Author(s): Nakayama, M.
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology, International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌
• [Presentation] Roles of the extracytoplasmic function sigma factors in Porphyromonas gingivalis (2011)
  • Author(s): Ohara, N.
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology, International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌
• [Presentation] Porphyromonas gingivalis HbRによる歯肉上皮細胞のIL-8産生機序 (2011)
  • Author(s): 大原直也
  • Organizer: 第64回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山
• [Presentation] 歯肉上皮細胞の歯周病原細菌取り込みに対するキトサンの影響 (2011)
  • Author(s): 大原直子
  • Organizer: 第64回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山
• [Presentation] マウスモデルから考える歯周病原細菌の病原性 (2011)
  • Author(s): 大原直也
  • Organizer: 第81回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 北九州
• [Presentation] Porphyromonas gingivalisによる破骨細胞分化抑制の分子基盤 (2010)
  • Author(s): 中山真彰
  • Organizer: 第63回日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山市
  • Year and Date: 2010-10-17
• [Presentation] 歯周病原細菌Porphyromonas gingivalisの新たな病原性に関する研究.特別講演 (2010)
  • Author(s): 大原直也
  • Organizer: 第31回岡山歯学会総会・学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-09-26
• [Presentation] 歯周病原細菌Porphyromonas gingivalisの新たな病原性に関する研究 (2010)
  • Author(s): 大原直也
  • Organizer: 第31回岡山歯学会総会・学術集会
  • Place of Presentation: 岡山市(招待講演)
  • Year and Date: 2010-09-26
• [Presentation] 歯周病原細菌およびその病原性因子の全身疾患への影響
  • Author(s): 大原直也
  • Organizer: 第60回日本化学療法学会学術集会
  • Place of Presentation: 長崎ブリックホール（長崎市）
  • Invited
• [Presentation] New aspect of signaling events in Porphyromonas gingivalis pathogenesis
  • Author(s): 大原直也
  • Organizer: First International Conference on Porphyromonas gingivalis and related Bacterial Species
  • Place of Presentation: 長崎大学良順会館（長崎市）
  • Invited
• [Presentation] Porphyromonas gingivalisによるAkt/GSK3beta pathwayの制御
  • Author(s): 中山真彰，井上哲圭，大原直也
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 奥羽大学（郡山市）