DNA methylation and histone modification regulate differentiationof skeletal stem cells
| Project/Area Number |
22390344
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
NIFUJI Akira 鶴見大学, 歯学部, 教授 (00240747)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Tatsuya 鶴見大学, 歯学部, 助教 (90323708)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 細胞分化 / 骨格組織 / 骨格系細胞 / ヒストン / メチル化 |
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| Research Abstract |
Chromatin structure is a critical determinant to regulate gene expression and it alters during fate decision or/and cell differentiation. Patterns of post-translational modification at histone tails as well as methylation patterns of cytosine residues at DNA affect chromatin structure. In this study, we examined histone modification during skeletal cell differentiation. We observed dynamic changes of expression of H3K9methyltransferases at H3K9 and localization of three H3K9 methylation patterns, me1, me2, and me3 , during skeletal development. It also suggests that nhibition of H3K9 me2 affects gene expression and subsequent cell differentiation.
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Report
(4 results)
Research Products
(48 results)
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[Presentation] Limited and comparable immunogenicity of terminally differentiated cells derived from both iPSCs and ESCs.2012
Author(s)
Uda M, Araki R, Hoki Y, Sayama M, Nakamura M, Andou S, Sugiura M, IdenoH, Shimada A, Nifuji A, Abe M.
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場、 福岡.
Year and Date
2012-12-13
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