| Project/Area Number |
22390345
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO Yukinari 東北大学, 医学部, 教授 (00571811)
TANIGUCHI Kunihisa 福岡歯科大学, 歯学部, 教授 (90105685)
TSURUGA Eichi 福岡歯科大学, 歯学部, 教授 (30295901)
KAJIYA Hiroshi 福岡歯科大学, 歯学部, 講師 (80177378)
HATAKEYAMA Yuji 福岡歯科大学, 歯学部, 講師 (40302161)
OKA Kyoko 福岡歯科大学, 歯学部, 講師 (60452778)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
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| Keywords | 口腔解剖学(含組織学発生学) / 微小循環 / 口腔扁平上皮癌 / ポドプラニン / 抗体 / 転移 / 細胞接着 / リンパ管内皮細胞 / 血小板 / CLEC2 / PMab-1 / NZ-1 / 唾液腺 / 脈絡叢 / PLAG / 癌幹細胞 / 遺伝子操作マウス |
|---|
| Research Abstract |
We developed novel antibodies specific for human and mouse podoplanin (PMab-1,NZ-1.2) sold in MBL, Imgenex, Sigma, and Millipore. Immunohistochemical studies on the oral squamous cell carcinoma (SCC) revealed that platelets exist between SCC and lymphatic endothelial cells at the luminal side of lymphatic vessels where SCC attached. The platelets may contribute to the lymphogenous metastasis of SCC. The adhesion activity to human lymphatic endothelial cells was higher in podoplanin-positive SCC than in podoplanin-negative SCC, and the adhesion of SCC was blocked by NZ-1.2. Anti-podoplanin may be useful for the inhibition of the lymphogenous metastasis of SCC.
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