Analyses of strengthening mechanisms of human dentin by UV

• Project/Area Number: 22390358
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: HAYASHI Mikako 大阪大学, 歯学研究科(研究院), 教授 (40271027)
• Co-Investigator(Kenkyū-buntansha): IWAMI Yukiteru 大阪大学, 大学院・歯学研究科, 助教 (90303982)
• Co-Investigator(Renkei-kenkyūsha): 箕島 弘二 大阪大学, 工学 (系)研究科(研究院), 教授 (50174107) ; 斎藤 充 東京慈恵会医科大学, 医学部, 准教授 (50301528)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 保存修復学 / 象牙質 / 機械的強度 / 紫外線 / コラーゲン / 歯学 / 曲げ強さ / 破壊靭性値 / ナノインデンテーション / 原子間力顕微鏡

Research Abstract

The strengthening effects of human dentin by UV irradiation were investigated with an aim of preventing tooth fracture. We found that a UV irradiation treatment with a wave length of 365 nm was significantly effective to improve the mechanical strengths of dentin with showing anisotolopy. The strengthening mechanism of dentin by UV was found to be the chemical changes in its type I collagen such as formation of new cross-links in relation with its typical amino acid such as proline.

Research Products

• [Journal Article] Detection of dentinal microcracks using infrared thermography (2013)
  • Author(s): Matsusita-Tokugawa M
  • Journal Title: Journal of Endodontics Volume: 39 Issue: 1 Pages: 88-91
  • DOI: 10.1016/j.joen.2012.06.033
  • Peer Reviewed
• [Journal Article] Activation of the Wnt/β-catenin pathway and tissue inhibitor of metalloprotease 1 during tertiary dentinogenesis (2013)
  • Author(s): Yoshioka S
  • Journal Title: Journal of Biochemistry Volume: 153 Issue: 1 Pages: 43-50
  • DOI: 10.1093/jb/mvs117
  • Peer Reviewed
• [Journal Article] Effects of heating on the mechanical and chemical properties of human dentin (2012)
  • Author(s): Hayashi M, Furuya Y, Minoshima K, Saito M, Marumo K, NakashimaS, HongoC, KimJ, Ota T, Ebisu S
  • Journal Title: Dental Materials Volume: 28 Issue: 4 Pages: 385-391
  • DOI: 10.1016/j.dental.2011.11.015
  • Peer Reviewed
• [Journal Article] Clinical guidelines for treating caries in adults following a minimal intervation policy-Evidence and consensus based report. (2012)
  • Author(s): Momoi Y, Hayashi M, 他
  • Journal Title: Journal of Dentistry Volume: 40 Pages: 95-105
  • Peer Reviewed
• [Journal Article] How regular visits and preventive programs affect onset of adult caries (2012)
  • Author(s): Ito A, Hayashi M, Hamasaki T, Ebisu S
  • Journal Title: Journal of Dental Research Volume: 91 Issue: 7_suppl Pages: S52-S58
  • DOI: 10.1177/0022034511435701
  • Peer Reviewed
• [Journal Article] Clinical guidelines for treating caries in adults following a minimal intervation policy-Evidence and consensus based report (2012)
  • Author(s): Momoi Y
  • Journal Title: Journal of Dentistry Volume: 40 Pages: 95-105
  • Peer Reviewed
• [Journal Article] Bone Marrow-Derived HipOP Cell Population Is Markedly Enriched in Osteoprogenitors (2012)
  • Author(s): Ito S
  • Journal Title: International Journal of Molecular Science Volume: 13 Issue: 8 Pages: 10229-10235
  • DOI: 10.3390/ijms130810229
  • Peer Reviewed
• [Journal Article] Clinical guidelines for treating caries in adults following a minimal intervention policy-Evidence and consensus based report (2012)
  • Author(s): Hayashi, et.al.
  • Journal Title: Journal of Dentistry Volume: 40 Issue: 2 Pages: 95-105
  • DOI: 10.1016/j.jdent.2011.10.011
  • NAID: 120006986655
  • Peer Reviewed
• [Journal Article] Risk assessment of dental caries by using Classification and Regression Trees (2011)
  • Author(s): Ito A, Hayashi M, Hamasaki T, Ebisu S
  • Journal Title: Journal of Dentistry Volume: 39 Pages: 457-463
  • Peer Reviewed
• [Journal Article] Ways of enhancing pulp preservation by stepwise excavation -A systematic review (2011)
  • Author(s): Hayashi M, Fujitani M, Yamaki C, Momoi Y
  • Journal Title: Journal of Dentistry Volume: 39 Issue: 2 Pages: 95-107
  • DOI: 10.1016/j.jdent.2010.10.012
  • Peer Reviewed
• [Journal Article] Relationship between laser fluorescence and bacterial invasion in arrested dentinal carious lesions (2011)
  • Author(s): Iwami Y, Yamamoto H, Hayashi M, Ebisu S
  • Journal Title: Lasers in Medicine and Science Volume: 26 Issue: 4 Pages: 439-444
  • DOI: 10.1007/s10103-010-0798-5
  • Peer Reviewed
• [Journal Article] Risk assessment of dental caries by using Classification and Regressi on Trees (2011)
  • Author(s): Hayashi, et.al
  • Journal Title: Journal of Dentistry Volume: 39 Issue: 6 Pages: 457-463
  • DOI: 10.1016/j.jdent.2011.04.002
  • Peer Reviewed
• [Journal Article] In deep cavities stepwise excavation of caries can preserve the pulp (2011)
  • Author(s): Hayashi, et.al.
  • Journal Title: Evidence Based Dentistry Volume: 12 Issue: 2 Pages: 68-68
  • DOI: 10.1038/sj.ebd.6400801
  • Peer Reviewed
• [Journal Article] 根管治療を再考する-その成功と失敗の鍵- (2011)
  • Author(s): 林美加子, 他
  • Journal Title: 日本歯内療法学雑誌 Volume: 32 Pages: 87-96
  • NAID: 10029089649
  • Peer Reviewed
• [Journal Article] 臼歯生活歯の歯冠色修復のオプション (2011)
  • Author(s): 林美加子
  • Journal Title: 日本歯科評論 Volume: 829 Pages: 47-54
• [Journal Article] Effects of rehydration on dentin strengthened by heating or UV irradiation (2010)
  • Author(s): Hayashi M, Okamura K, Koytchev EV, Furuya Y, Sugeta A, Ota T, Ebisu S
  • Journal Title: Journal of Dental Research Volume: 89 Issue: 2 Pages: 154-158
  • DOI: 10.1177/0022034509354564
  • Peer Reviewed
• [Journal Article] Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials (2010)
  • Author(s): Imazato S, Hayashi M,他
  • Journal Title: Dental Materials Journal Volume: 29 Pages: 341-346
  • NAID: 10026470432
  • Peer Reviewed
• [Journal Article] Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials (2010)
  • Author(s): Imazato, Hayashi, et.al.
  • Journal Title: Dental Material Journal Volume: 29 Pages: 341-346
  • NAID: 10026470432
  • Peer Reviewed
• [Journal Article] Relationship between laser fluorescence and bacterial invasion in arrested dentinal carious lesions (2010)
  • Author(s): Iwami, Hayashi, et.al.
  • Journal Title: Lasers in Medical Science Volume: 89 Pages: 154-158
  • Peer Reviewed
• [Journal Article] 失活歯強化法の開発と加熱および紫外線による象牙質強化メカニズムの解明 (2010)
  • Author(s): 林美加子
  • Journal Title: 大阪大学歯学会雑誌 Volume: 55 Pages: 1-6
• [Journal Article] 審美歯科治療における歯の寿命を考えた処置 (2010)
  • Author(s): 林美加子
  • Journal Title: 歯科審美 Volume: 23 Pages: 18-20
  • NAID: 10026709048
• [Presentation] Functional analysis of Leukemia Inhibitory Factor in osteoblast differentiation of bone marrow stromal cells (2012)
  • Author(s): Matsushita K, Itoh S, Ikeda S, Yamamoto Y, Yamauchi Y, Hayashi M
  • Organizer: Internathional Conference on Progress in Bone and Mineral Reserch 2012
  • Place of Presentation: Vienna, Austria.
  • Year and Date: 2012-11-29
• [Presentation] バイオメカニクスの視点でとらえる高齢者の歯根破折 (2012)
  • Author(s): 林美加子
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-22
• [Presentation] 慢性う蝕の進行状態の評価におけるう蝕歯質の硬さ測定の有用性 (2012)
  • Author(s): 岩見行晃,山本洋子,林美加子
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-22
• [Presentation] Effect of Degraded Dentin Matrix Proteins induced by Matrix Metalloproteinase Molecules on Pulp Cells Function (2012)
  • Author(s): Takahashi Y, Okamoto M, Yoshioka S, Hayashi M
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-22
• [Presentation] Development of rechargeable CPC-loaded hydrogel particles for long-term delivery of antimicrobials (2012)
  • Author(s): Kitagawa H, Imazato S, Hayashi M
  • Organizer: The First Japan-Thailand-Korea Joint Symposium on Translational Research in Oral Sciences
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2012-11-16
• [Presentation] 口腔外傷治療最前線「歯根破折への保存的対応 vs 外科的対応」 (2012)
  • Author(s): 林美加子
  • Organizer: 日本歯科医学会総会分科会プログラム (日本外傷歯学会)
  • Place of Presentation: 大阪市
  • Year and Date: 2012-11-12
• [Presentation] 臨学一体の日本歯内療法学会がお知らせする最新の歯内療法「先進医療: X線CT画像診断に基づく手術用顕微鏡を用いた歯根端切除手術」 (2012)
  • Author(s): 林美加子
  • Organizer: 日本歯科医学会総会分科会プログラム (日本歯内療法学会)
  • Place of Presentation: 大阪市
  • Year and Date: 2012-11-11
• [Presentation] Dispersal of Porphyromonas gingivalis biofilm induced by environmental factors and proteases. (2012)
  • Author(s): Yamaguchi M, Noiri Y, Asahi Y, Maezono H, Yamamoto R, Kuremoto K, Hayashi M, Ebisu S
  • Organizer: 6th ASM Conference on Biofilms
  • Place of Presentation: Miami, USA
  • Year and Date: 2012-10-03
• [Presentation] 紫外線照射による象牙質強化効果メカニズムの化学的解析 (2012)
  • Author(s): 古谷優,武田侑子,和田陽子,辻本恭久,林美加子,恵比須繁之
  • Organizer: 第136回日本歯科保存学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-29
• [Presentation] 光透過型ファイバーポスト修復の根部における接着状態の評価 (2012)
  • Author(s): 熊 宇,古谷優, 武田侑子,林美加子,今里聡
  • Organizer: 第136回日本歯科保存学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-29
• [Presentation] 象牙質の構成成分および微細構造における個体差が機械的性質に及ぼす影響 (2012)
  • Author(s): 武田侑子,古谷優,石本卓也,中野貴由,林美加子
  • Organizer: 第136回日本歯科保存学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-28
• [Presentation] Chemical changes through UV strengthening of human dentin. (2012)
  • Author(s): Furuya Y, Takeda Y, Hayashi M
  • Organizer: 90th International Association for Dental Research General Session
  • Place of Presentation: Iguasu, Brazil
  • Year and Date: 2012-06-20
• [Presentation] Best ways to treat pulpless teeth Practical strategies to prevent root fracture (2012)
  • Author(s): Hayashi M
  • Organizer: International Symposium for Adhesive Dentistry
  • Place of Presentation: Tokyo
  • Year and Date: 2012-05-19
• [Presentation] How to achieve the best oral cosmetic appearance while preserving maximum natural tissues. (2012)
  • Author(s): Hayashi M
  • Organizer: 28th American Academy of Cosmetic Dentistry
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2012-05-04
• [Presentation] ow to achieve the best oral cosmetic appearance while preserving maximum natural tissues (2012)
  • Author(s): Hayashi M
  • Organizer: 28th American Academy of Cosmetic Dentistry
  • Place of Presentation: Washington DC
  • Invited
• [Presentation] Chemical changes through UV strengthening of human dentin (2012)
  • Author(s): Furuya Y
  • Organizer: 90th International Association for Dental Research General Session
  • Place of Presentation: Iguasu
• [Presentation] Dispersal of Porphyromonas gingivalis biofilm induced by environmental factors and proteases (2012)
  • Author(s): Yamaguchi M
  • Organizer: 6th ASM Conference on Biofilms
  • Place of Presentation: Miami
• [Presentation] う蝕病巣におけるう蝕歯質の硬さと DIAGNOdentによる評価との関係 (2011)
  • Author(s): 岩見 行晃,山本洋子,林美加子,恵比須繁之
  • Organizer: 第135回日本歯科保存学会
  • Place of Presentation: 大阪市
  • Year and Date: 2011-10-21
• [Presentation] 赤外線サーモグラフィを用いた歯根破折診断法の開発 (2011)
  • Author(s): 松下真美,三浦治郎,竹重文雄,岩見行晃,阪上隆英,和泉遊以,林美加子,今里聡,恵比須繁之
  • Organizer: 第135回日本歯科保存学会
  • Place of Presentation: 大阪市
  • Year and Date: 2011-10-20
• [Presentation] Risk assessment of dental caries in adult patients (2011)
  • Author(s): Hayashi M, Ito A, Ebisu S
  • Organizer: 88th European Organisation for Caries Research
  • Place of Presentation: Kaunas, Lithuania
  • Year and Date: 2011-07-09
• [Presentation] Risk assessment of dental caries in adult patients using multiple logistic regression analysis (2011)
  • Author(s): Hayashi M, et.al.
  • Organizer: 88^<th> European Organisation for Caries Research
  • Place of Presentation: Kaunas
  • Year and Date: 2011-07-09
• [Presentation] Survey of how relevant ICDAS-II is to Japanese general practitioners who focus on preventive dentistry (2011)
  • Author(s): Sugiyama S, Hayashi M, et.al.
  • Organizer: 88^<th> European Organisation for Caries Research
  • Place of Presentation: Kaunas
  • Year and Date: 2011-07-08
• [Presentation] 波長405nm紫外線照射による象牙質強化効果の検討 (2011)
  • Author(s): 武田侑子, 林美加子, 古谷優, 恵比須繁之
  • Organizer: 第134回日本歯科保存学会
  • Place of Presentation: 浦安市
  • Year and Date: 2011-06-10
• [Presentation] Analysis of strengthening mechanisms of human dentin by UV irradiation. (2011)
  • Author(s): Furuya Y, Hayashi M, Takeda Y, Ebisu S
  • Organizer: International Dental Materials Congress 2011
  • Place of Presentation: Seoul, South Korea
  • Year and Date: 2011-05-29
• [Presentation] Analyses of stress distributions and fracture strength of pulpless teeth restored with fiber posts (2011)
  • Author(s): Takeda Y, Furuya Y, Hayashi M, Ebisu S
  • Organizer: International Dental Materials Congress 2011
  • Place of Presentation: Seoul, South Korea
  • Year and Date: 2011-05-29
• [Presentation] Analysis of strengthening mechanisms of human dentin by UV irradiation (2011)
  • Author(s): Furuya Y, Hayashi M, et.al.
  • Organizer: International Materials Congress 2011
  • Place of Presentation: Seoul
  • Year and Date: 2011-05-29
• [Presentation] Analyses of stress distributions and fracture strength of pulpless teeth restored with fiber post (2011)
  • Author(s): Takeda Y, Hayashi M, et.al.
  • Organizer: International Materials Congress 2011
  • Place of Presentation: Seoul
  • Year and Date: 2011-05-28
• [Presentation] Modern caries treatments with the aim of protecting and preserving teeth (2011)
  • Author(s): Hayashi M
  • Organizer: 4^<th> World Society of Lingual Orthodontic Congress
  • Place of Presentation: Osaka(招待講演)
  • Year and Date: 2011-04-03
• [Presentation] Analysis of mechanical properties of human dentin with UV irradiation (2010)
  • Author(s): Furuya Y, Hayashi M, Ebisu S
  • Organizer: Osaka- Chulalongkorn-Mahidol Joint Symposium
  • Place of Presentation: Suita
  • Year and Date: 2010-11-26
• [Presentation] Caries risk assessment using data mining approach (2010)
  • Author(s): Hayashi M, Ito A, Ebisu S
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] UV strengthens human dentin under rehydration condition (2010)
  • Author(s): Furuya Y, Hayashi M, Ebisu S
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] UV strengthens human dentin under rehydration condition (2010)
  • Author(s): Furuya, Y., Hayashi, M., Ebisu
  • Organizer: The 88^<th> International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Caries risk assessment using data mining approach (2010)
  • Author(s): Hayashi, M., Ito, A., Ebisu, S
  • Organizer: The 88^<th> International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] 紫外線照射による象牙質の機械的強度変化の多面的解析 (2010)
  • Author(s): 古谷優, 林美加子,恵比須繁之
  • Organizer: 第132回日本歯科保存学会
  • Place of Presentation: 熊本市
  • Year and Date: 2010-06-04
• [Presentation] 紫外線照射による象牙質の機械的強度変化の多面的解析 (2010)
  • Author(s): 古谷優、林美加子、恵比須繁之
  • Organizer: 第132回日本歯科保存学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-04
• [Presentation] データマイニングの手法を用いたう蝕病変発生予測モデルの構築 (2010)
  • Author(s): 伊藤中、林美加子、濱崎俊光、恵比須繁之
  • Organizer: 第132回日本歯科保存学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-04
• [Book] 削るう蝕・削らないう蝕 (2012)
  • Author(s): 今里聡 , 林美加子 , 伊藤中
  • Publisher: クインテッセンス出版
• [Book] 削るう蝕・削らないう蝕 (2012)
  • Author(s): 今里 聡
  • Total Pages: 215
  • Publisher: クインテッセンス
• [Patent(Industrial Property Rights)] 歯科装置 (2012)
  • Inventor(s): 林 美加子,恵比須繁之
  • Industrial Property Rights Holder: 大阪大学
  • Acquisition Date: 2012-09-09