Development of regenerative medicine using human iPS cellsFor pulp and periapical pathoses

• Project/Area Number: 22390360
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: HAYASHI Yoshihiko 長崎大学, 大学院・医歯薬学総合研究科, 教授 (20150477)
• Co-Investigator(Kenkyū-buntansha): YAMADA Shizuka 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00363458)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 再生医療 / iPS細胞 / 低酸素培養 / 細胞増殖・分化 / 骨芽細胞誘導

Research Abstract

There are no stastical differences in either the growth and differentiation of (induced pluripotent stem) iPS cells or the induction of iPS cells to osteoblasts between the four and three transcription factor froups cultured under hypoxia. Furthermore, the function of (hypoxia inducible factors) HIFs in murine iPS cells under hypoxia was investigated in relation to the morphology and expression of the transcription factors. The HIF-2α knockdown group exhibited a decreased colony size in the iPS cells. The HIF-2α or -3α demonstrated a statistical significant decrease in the expression of the transcription factors compared to those observed in the control group. These results demonstrate that HIF-2α among HIFs is the most influential candidate for the maintenance of the pluripotency of murine iPS cells.

Research Products

• [Journal Article] Effects of glucosamine on the tooth pulpal nociceptive responses in the rat (2013)
  • Author(s): Kaida K, Yamashita H, Toda K, Hayashi Y.
  • Journal Title: Journal of Dental Sciences Volume: 8 Pages: 68-73
• [Journal Article] Effects of fish collagen peptides on collagen post-translational modifications and mineralization in an osteoblastic cell culture system (2013)
  • Author(s): Yamada S, Nagaoka H, Terajima M, Tsuda N, Hayashi Y, Yamauchi M.
  • Journal Title: Dental Materials Journal Volume: 32 Pages: 1-8
  • NAID: 10031138706
• [Journal Article] Challenges to the clinical placement and evaluation of adhesively-bonded, cervical composite restorations (2013)
  • Author(s): Kubo S, Yokota H, Yokota H, Hayashi Y.
  • Journal Title: Dent Mater Volume: 29(1) Pages: 10-27
  • NAID: 120006986667
• [Journal Article] Early gene and protein expression associated with osteoblast proliferation and differentiation in response to fish collagen peptides (2013)
  • Author(s): Yamada S, Yoshizawa Y, Kawakubo A, Ikeda T, Yanagiguchi K, Hayashi Y
  • Journal Title: Dental Materials Journal Volume: 32 Pages: 233-240
• [Journal Article] Effects of glucosamine on the tooth pulpal nociceptive responses in the rat. (2013)
  • Author(s): Kaida K
  • Journal Title: Journal of Dental Sciences Volume: 8 Pages: 68-73
  • Peer Reviewed
• [Journal Article] Effects of fish collagen peptides on collagen post-translational modifications and mineralization in an osteoblastic cell culture system. (2013)
  • Author(s): Yamada S
  • Journal Title: Dental Materials Journal Volume: 32 Pages: 1-8
  • NAID: 10031138706
  • Peer Reviewed
• [Journal Article] Challenges to the clinical placement and evaluation of adhesively-bonded, cervical composite restorations. (2013)
  • Author(s): Kubo S
  • Journal Title: Dent Materials Volume: 29 Pages: 10-27
  • Peer Reviewed
• [Journal Article] Early gene and protein expression associated with osteoblast proliferation and differentiation in response to fish collagen peptides. (2013)
  • Author(s): Yamada S
  • Journal Title: Dental Materials Journal Volume: 32 Pages: 233-240
  • Peer Reviewed
• [Journal Article] Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation (2012)
  • Author(s): Kawasaki A, Hayashi Y, Yanagiguchi K, Yamada S, Syudo M, Igawa K, Ikeda T, Kubo S, Fujiwara M.
  • Journal Title: Journal of dental Sciences Volume: 7 Pages: 94-98
  • NAID: 120006985389
• [Journal Article] Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation. (2012)
  • Author(s): Kawasaki A
  • Journal Title: Journal of Dental Sciences Volume: 7 Pages: 94-98
  • Peer Reviewed
• [Journal Article] Zinc as as essential trace element in the acceleration of matrix vesicles-mediated mineral deposition (2011)
  • Author(s): Kawakubo A, Matsunaga T, Ishizaki H, Yamada S, Hayashi Y.
  • Journal Title: Microscopy research and techniques Volume: 74 Pages: 1161-1165
• [Journal Article] 根管充填用レジンシーラーの生体親和性 (2011)
  • Author(s): 柳口嘉治郎、首藤 実、川崎 綾、杉本浩司、池田 毅、山田志津香、林 善彦
  • Journal Title: 本歯内療法学会雑誌 Volume: 32(3) Pages: 212-216
  • NAID: 10029697632
• [Presentation] マウスiPS 細胞の低酸素下培養におけるHIF(Hypoxia Inducible factor) の役割 (2012)
  • Author(s): 杉本浩司,吉澤 有,石崎秀隆,林 善彦
  • Organizer: 第136回日本歯科保存学会春季学術大会
  • Place of Presentation: 宜野湾市
• [Presentation] 歯髄再生に使用する足場材 (2012)
  • Author(s): 林 善彦
  • Organizer: 第10回日本再生歯科医学会
  • Place of Presentation: 神戸市
• [Presentation] 低酸素下におけるiPS細胞の増殖・分化挙動 (2011)
  • Author(s): 杉本浩司, 石崎秀隆, 池田毅, 林善彦
  • Organizer: 第32回日本歯内療法学会学術大会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-07-30
• [Presentation] 因子・4因子導入マウスiPS細胞の低酸素培養における増殖・分化について (2011)
  • Author(s): 杉本浩司, 石崎秀隆, 林善彦
  • Organizer: 第134回日本歯科保存学会春季大会
  • Place of Presentation: 浦安市
  • Year and Date: 2011-06-10
• [Presentation] 3因子・4因子導入マウスiPS細胞の低酸素培養における増殖・分化について (2011)
  • Author(s): 杉本浩司,石崎秀隆,林 善彦
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 浦安市
• [Presentation] 低酸素下におけるPS細胞の増殖・分化挙動 (2011)
  • Author(s): 杉本浩司,石崎秀隆,池田 毅、林 善彦
  • Organizer: 第32回日本歯内療法学会学術大会
  • Place of Presentation: 長崎市
• [Presentation] マウスiPS細胞の低酸素下培養におけるHIF(Hypoxia Inducible factor) の役割
  • Author(s): 林 善彦
  • Organizer: 第136回日本歯科保存学会春季学術大会
  • Place of Presentation: 宜野湾市
• [Presentation] 歯髄再生に使用する足場材
  • Author(s): 林 善彦
  • Organizer: 第10回日本再生歯科医学会
  • Place of Presentation: 神戸市
  • Invited
• [Book] Vhapter 16 Chitosan Application in Dentistry. In Marine Nutraceuticals: Prospects and perspective (2013)
  • Author(s): Hayashi Y, Yanagiguchi K, Koyama Z, Ikeda T, Yamada S
• [Book] Marine Nutraceuticals: Prospects and Perapectives (2013)
  • Author(s): Hayashi Y
  • Total Pages: 444
  • Publisher: CRC Press
• [Book] Chapter 6 Chitosan and fish collagen as biomaterials for regenerative medicine. In Marine Medical Food, Volume 65, AFNR (2012)
  • Author(s): Hayashi Y, Yamada S, Yanagiguchi K, Koyama Z, Ikeda T.
  • Publisher: ed. Se-Kwon Kim, UK: Academic Press
• [Book] Advances in Food and Nutrition Research Volume 65 (2012)
  • Author(s): Hayashi Y
  • Total Pages: 523
  • Publisher: Academic Press
• [Book] Marine Cosmeceuticals: Trends and Prospects (2012)
  • Author(s): Hayashi Y
  • Total Pages: 412
  • Publisher: CRC Press
• [Book] Marine Medical Food, Volume 65, Advances in Food and Nutrition Research (2012)
  • Author(s): Hayashi Y, Yamada S, Yanagiguchi K, Koyama Z, Ikeda T(分担)
  • Total Pages: 523
  • Publisher: Academic Press
• [Book] Fish collagen and tissue repair. In "Marine Cosmeceuticals: Trends and Prospects" (2011)
  • Author(s): Hayashi Y, Yamada S, Ikeda T, Yanagiguchi K.
  • Publisher: ed. S-K. Kim, CRC Press, Boca Raton, FL
• [Book] Marine Cosmeceuticals : Trends and Prospects (2011)
  • Author(s): Hayashi Y, Yamada S, Ikeda T, Yanagiguchi K(分担)
  • Total Pages: 412
  • Publisher: CRC Press
• [Book] Chitin, Chitosan, Oligosaccharides and Their Derivatives分担 (2011)
  • Author(s): Yoshihiko Hayashi
  • Publisher: CRC Press (Taylor & Francis Group)
• [Book] Application of chiosan oligosaccharide and glucosamine in dentistry. In Chitin, Chitosan, Oligosaccharides and Their Derivatives: Biological Activities and Applications (2010)
  • Author(s): Hayashi Y.
  • Publisher: Se-Kwon Kim, CRC Press-Taylor & Francis Group