Development of regenerative medicine using human iPS cellsFor pulp and periapical pathoses
| Project/Area Number |
22390360
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HAYASHI Yoshihiko 長崎大学, 大学院・医歯薬学総合研究科, 教授 (20150477)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Shizuka 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00363458)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | 再生医療 / iPS細胞 / 低酸素培養 / 細胞増殖・分化 / 骨芽細胞誘導 |
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| Research Abstract |
There are no stastical differences in either the growth and differentiation of (induced pluripotent stem) iPS cells or the induction of iPS cells to osteoblasts between the four and three transcription factor froups cultured under hypoxia. Furthermore, the function of (hypoxia inducible factors) HIFs in murine iPS cells under hypoxia was investigated in relation to the morphology and expression of the transcription factors. The HIF-2α knockdown group exhibited a decreased colony size in the iPS cells. The HIF-2α or -3α demonstrated a statistical significant decrease in the expression of the transcription factors compared to those observed in the control group. These results demonstrate that HIF-2α among HIFs is the most influential candidate for the maintenance of the pluripotency of murine iPS cells.
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Report
(4 results)
Research Products
(30 results)
