Development of an anti-aging protocol and maintaining undifferentiated state of oral mucosa keratinocyte progenitor/stem cells by pharmacological manipulation of autophagy

• Project/Area Number: 22390371
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Niigata University
• Principal Investigator: IZUMI Kenji 新潟大学, 医歯学系, 准教授 (80242436)
• Co-Investigator(Kenkyū-buntansha): MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941) ; TERADA Michiko 新潟大学, 医歯学系, 特任助教 (60374550)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: オートファジー / mTOR / ラパマイシン / 口腔粘膜上皮 / 前駆/幹細胞 / ウェスタンブロッティング / TASCC / 細胞老化 / 分化 / 分泌タンパク

Research Abstract

The TOR-autophagy spatial coupling compartment (TASCC) represents for the intracytoplasmic region where protein degradation and synthesis occur in unison to handle rapid protein turnover by integration of rough endoplasmic reticulum, Golgi apparatus, (auto)lysosomes and mTOR, which is beneficial to cellsto efficiently produce secretory proteins. In this study, we found the TASCC in primary cultured human oral keratinocytes. In addition, our results suggested Maspin and IL-6 produced by oral keratinocyteswere synthesized and secreted through TASCC.

Research Products

• [Journal Article] Enrichment of oral mucosa and skin keratinocyte progenitor/stem cells (2013)
  • Author(s): Izumi K, Marcelo CL, Feinberg SE
  • Journal Title: Methods Mol Biol Volume: 989 Pages: 293-303
  • Peer Reviewed
• [Journal Article] Enrichment of oral mucosa and skin keratinocyte progenitor/stem cells. (2013)
  • Author(s): （Izumi K）, Marcelo CL, Feinberg SE.
  • Journal Title: Methods Mol Biol Volume: 989 Pages: 293-303
  • DOI: 10.1007/978-1-62703-330-5_23
  • ISBN: 9781627033299, 9781627033305
  • Peer Reviewed
• [Presentation] 培養口腔粘膜上皮細胞における細胞分化とTASCCに関する検討 (2013)
  • Author(s): 泉健次,加藤寛子,大貫尚志,齋藤太郎,塩見晶,上野山敦士,寺田典子,河野芳朗,野澤-井上佳世子,前田健康
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松
• [Presentation] 培養口腔粘膜上皮細胞における細胞分化とTASCCに関する検討 (2013)
  • Author(s): （泉 健次）、加藤寛子、大貫尚志、齋藤太郎、塩見 晶、上野山敦士、寺田典子、河野芳朗、野澤-井上佳世子、前田健康
  • Organizer: 第118回日本解剖学会総会
  • Place of Presentation: 高松国際会議場（高松市）
• [Presentation] Clinical Trials in TE/RM : Oral Mucosa (2011)
  • Author(s): 泉健次
  • Organizer: 4th International Conference on Biomedical Engineering
  • Place of Presentation: Ho Chi Minh City(招待講演)
  • Year and Date: 2011-01-10