| Project/Area Number |
22390374
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
EGAMI Youhei 香川大学, 医学部, 助教 (80432780)
MATSUDA Chie 独立行政法人産業技術総合研究所, バイオメディカル部門, 主任研究員 (50344099)
KANAGAWA Motoi 神戸大学, 医学研究科, 助教 (00448044)
TANAKA Toru 城西大学, 薬学部, 准教授 (60217049)
FUKAI Naomi 奥羽大学, 歯学部, 教授 (60134681)
YOSHIYAMA Masahiro 岡山大学, 歯学部, 教授 (10201071)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAKI Shinichi 香川大学, 医学部, 教授 (10202748)
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 細胞膜修復 / 細胞膜損傷 / Annexin / MICAL / dysferlin / 筋ジストロフィー / 二光子 / ライブイメージング / エクトゾーム / 小胞輸送 / MICAL1 / アネキシン / 膜融合 / 二光子レーザー |
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| Outline of Final Research Achievements |
Plasma membrane disruption is a common form of cell injury in mammalian tissues under physiological conditions. We expressed MICAL1-, Annexins-,MG53-, dyferlin-GFPs in culture cells or in vivo, and then subjected them to a plasma membrane disruption created by a two-photon laser. Subsequent confocal imaging with a high sensitive detector unit revealed more striking wave and faster (second time-scale) accumulation of MICAL1-GFP at the disruption site comparing to MG53 or dysferlin-GFPs, followed by actin-RFP depolymerization (second time-scale). We also observed, for the first time in culture cells or living skeletal muscle cells responding to a membrane disruption, subsequent confocal imaging revealed striking accumulation of annexins (A1, A2, A4, A5, A6, A7, S100A10, S100A11)-GFPs at the disruption site. The membrane repair mechanism is now well learned at the cellular and molecular level by multi-photon laser microscope with a high sensitive detector unit.
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