Investigation the mechanisms on invasion and metastasisof oral squamous cell carcinoma
| Project/Area Number |
22390382
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWASHIRI Shuichi 金沢大学, 医学系, 教授 (30291371)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Keywords | 口腔扁平上皮癌 / 浸潤 / 転移 / 接着分子 / 基質分解酵素 / 血管新生 / 間質 / 増殖 / 細胞運動 / 基質分解 / 酵素 / リンパ管新生 / 運動 / 接着 |
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| Research Abstract |
We have been studying the mode of cancer invasion as the important prognostic factor in patients with oral squamous cell cancer. Grade 4 of mode of invasion proposed by Jakobsson was subclassified into grades 4C (Cord-like type) and 4D (Diffuse type). The purpose of this study is to immunohistochemically and experimentally elucidate this modified grading system for the mode of invasion. Materials and methods used are human cancer materials and in vitro and in vivo invasion model by using 3 cell lines; OCS-20 (Gr.3), OSC-19 (Gr. 4C) and HOC-313 (Gr. 4D). In immunostained human cancer materials, the higher the grade of the mode of invasion was, the less continuous the type 4 collagen was, and the less E-cadherin was. Majority of diffuse invasive cancers (Gr. 4C and 4D) expressed MMPs and HIF-1. In cell invasion assay, HOC-313 showed the greatestmotility (AMF). Cell lines cultured on 3T3 fibroblast-embedded collagen gels showed similar invasion pattern to their origin. Among them, only HOC-313 showed invasion evenif fibroblast was not embedded. OSC-20 and OSC-19 implanted orthotopically in the tongue of nude mice grew and metastasized to lymph nodes. Their histologic appearance was similar to that of the origin. HOC-313 grew temporarily for several months by support of matrigel. In recent study, cell selected OLC-01 (Gr.4D) produced lethal tumorgenicity in nude mouse with similar histology to the origin.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Loss of claudin-7 is a negative prognostic factor for invasion andmetastasis in oral squamous cell carcinoma2013
Author(s)
Yoshizawa K., Nozaki S., Kato A., Hirai M., Yanase M., Yoshimoto T., KimuraI., Sugiura .S, Okamune A., Kitahara H., Noguchi N., Kato K., Ueki K., Kawashiri S
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Journal Title
Oncology Report
Volume: 29
Issue: 2
Pages: 445-450
DOI
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Peer Reviewed
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[Presentation] Evaluation of clinical and histopathological factors for survival in squamous cell carcinoma of oral cavity.2011
Author(s)
Kawashiri,S., Noguchi,N., Kato,K.,Yoshizawa,K., Kitahara,H., Okamune,A.,Sugiura,S., Yamamoto,E
Organizer
20th International Conference of Oral and Maxillofacial Surgery
Place of Presentation
Casa Piedra Centrode Eventos Congresos, Santiago (Chile)
Year and Date
2011-11-03
Related Report
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[Presentation] 口腔扁平上皮癌におけるMapin 発現とuPA/uPAR 発現-浸潤様式と予後との関連について-2011
Author(s)
吉澤邦夫,能崎晋一,八木瑞希,木村依世,杉浦史郎,岡宗絢子,北原寛子,加藤広禄,野口夏代,川尻秀一,中川清昌,山本悦秀
Organizer
第29回日本口腔腫瘍学会総会
Place of Presentation
崇城大学市民ホール(熊本県)
Year and Date
2011-01-27
Related Report
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[Presentation] Expression of Hypoxia-Inducible Factor-1 Alpha in oral squamous cell carcinoma2010
Author(s)
Noguchi, N., Kawashiri, S., Kato, K., Yoshizawa, K., Kitahara, H., Yamamoto, E
Organizer
XX Congress of the European Association of Cranio-Maxillo-Facial Surgery
Place of Presentation
Concertgebouw (Belgium)
Year and Date
2010-09-14
Related Report
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