Jaw bone regeneration using gene activated matrix incorporated withdirectly prepared adipose derived stem cells
| Project/Area Number |
22390387
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
ASAHINA Izumi 長崎大学, 医歯薬学総合研究科, 教授 (30221039)
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| Co-Investigator(Kenkyū-buntansha) |
SUMITA Yoshinori 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (50456654)
KOMORI Tosihisa 長崎大学, 大学院・医歯薬学総合研究科, 教授 (00252677)
NISHIMURA Masahiro 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00294570)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | 口腔顎顔面再健外科学 / 遺伝子活性化基質 / 遺伝子導入 / BMP4 / 脂肪由来幹細胞 / サケコラーゲン / リン酸カルシウム / 骨組織再生 / BMP / RunxII / 幹細胞移植 / 脂肪由来間葉系幹細胞 / 即時移植 / プラスミド遺伝子導入 / Runx II |
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| Research Abstract |
Adipose derived stem cells (ADSCs) have been isolated from adipose tissue by the simple method with collagenase digestion and centrifugation. Direct implantation of freshly isolated ADSCs with minimal amount of BMP could induce ectopic bone formation. ADSCs transfected with the plasmid carrying BMP and/or Wnt genes differentiated into osteoblasts in vitro. Implantation of the gene activated matrix (GAM) consisted with atelocollagen, which constructed with BMP gene by calcium precipitation method, induced bone formation and the addition of ADSCs to the GAM accelerated the bone induction.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Chronological histological changes during bone regeneration on a non-crosslinked atelocollagen matrix2012
Author(s)
Kagawa R, Kishino M, Sato S, Ishida K, Ogawa Y, Ikebe K, Oya K, Ishimoto T, Nakano T, Maeda Y, Komori T, Toyosawa S
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Journal Title
J Bone Miner Metab.
Volume: 30(6)
Pages: 638-50
NAID
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[Presentation] Growth/differentiation factors (GDFs) maintain tendongenic characteristics of human periodontal ligament-derived cells in culture2011
Author(s)
Sumita Y, Xia D, Wakamatsu Y, Kawasaki T, Liu Y, Maria O, Khalili S, Agata H, Kagami H, Tran SD, Asahina I
Organizer
9th Annual Meeting of International Society for Stem Cell Research
Place of Presentation
Toronto, Canada
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