Development of new anticancer drugs and nanobubbles suitable for specific molecular target therapy using sonoporation
| Project/Area Number |
22390389
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IWANAGA Kenjiro 九州歯科大学, 歯学部, 助教 (20448484)
TOMINAGA Kazuhiro 九州歯科大学, 歯学部, 教授 (40188793)
NISHIHARA Tatsuji 九州歯科大学, 歯学部, 教授 (80192251)
HABU Manabu 九州歯科大学, 歯学部, 助教 (00360058)
KODAMA Masaaki 九州歯科大学, 歯学部, 助教 (40423975)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHINO Norikazu 九州工業大学, 生命体工学研究科, 教授 (40145165)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | ソノポレーション / ナノバブル / 癌 / DDS / HDACi |
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| Research Abstract |
We investigated the effects on cytotoxicity by combination of a novel HDAC inhibitor, Ky-2, and bubble liposomes on oral squamous carcinoma cells in vitro. Cell viability was significantly reduced by treatment with Ky-2 at 25 nM and bubble liposomes and ultrasoud, while it also led to augmentation of the proportion of cells in the sub-G1 phase and DNA fragmentation. In addition, immunoblot analysis revealed that Ky-2 enhanced the expression of apoptosis-related proteins. We made new bubble liposomes from Doxil(R) and investigated the effects on cytotoxicity by combination of this new Doxil(R) bubble liposome (encapsulated doxorubicin bubble liposomes, EDBL) and ultrasound in human gingival squamous cell carcinoma Ca9-22 cells. Results showed that the effect on cytotoxity was enhanced by a combination of EDBL and ultrasound.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Mechanisms of G1 cell cycle arrest and apoptosis in myeloma cells induced by hybrid-compound histone deacetylase inhibitor2013
Author(s)
Fujii, S., Okinaga, T., Ariyoshi, W., Takahashi, O., Iwanaga, K., Nishino, N., Tominaga, K., Nishihara, T
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Journal Title
Biochem Biophys Res Commun434
Volume: 3
Pages: 413-20
Related Report
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[Journal Article] Mechanisms of G1 cell cycle arrest and apoptosis in myeloma cells induced by hybrid-compound histone deacetylase inhibitor2013
Author(s)
Fujii, S., Okinaga, T., Ariyoshi, W., Takahashi, O., Iwanaga, K., Nishino, N., Tominaga, K., Nishihara, T
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Journal Title
Biochem Biophys Res Commun
Volume: 434
Issue: 3
Pages: 413-20
DOI
Related Report
Peer Reviewed
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[Journal Article] Novel histone deacetylase inhibitor exhibits antitumor activity via apoptosis induction in oral squamous cell carcinoma2011
Author(s)
Takahashi,O.,Okinaga,T.,Iwanaga,K., Habu,M., Ariyoshi,W.,Tominaga,K. Nishino,N and Nishihara,T
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Journal Title
Journal of Biophysical Chemistry
Volume: Vol.2, No.3
Pages: 215-221
Related Report
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[Journal Article] Inhibition of adjuvant arthritis in rats by electroporation with interleukin-1 receptor antagonist2011
Author(s)
Sukedai,M., Ariyoshi, W., Okinaga, T, K. Iwanaga, M. Habu, I. Yoshioka, K. Tominaga, T. Nishihara
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Journal Title
J. Interferon and Cytokine Res.
Volume: Nov;31(11)
Pages: 839-46
Related Report
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