Investigation on the automation and speeding up ofmeasurement of the plasma IgG antibody titers against periodontopathic bacteria

• Project/Area Number: 22390397
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Okayama University
• Principal Investigator: TAKASHIBA Shogo 岡山大学, 大学院・医歯薬学総合研究科, 教授 (50226768)
• Co-Investigator(Kenkyū-buntansha): MAEDA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (00274001) ; OHARA Naoya 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70223930)
• Research Collaborator: NOZOE Mikio サンスター株式会社
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000); Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 歯学 / 細菌 / 感染症 / 免疫学 / プロテオーム / 歯周病 / 歯周病原性細菌 / 血漿IgG抗体価 / 特異抗原 / 臨床検査 / 血液 / IgG / 歯周病原細菌 / Porphyromonas gingivalis / 抗体価検査

Research Abstract

It is difficult to measure serum IgG antibody titers against periodontopathic bacteria stably and speedily. In this study, we havesucceeded to select antigens of Porphyromonas gingivalisfor effective serodiagnosis of periodontitis and synthesized 16 antigens. In addition, we confirmed the reaction against serum obtained from periodontal patients. Inthe future, we will identify antigens for effective serodiagnosis and establish the automatic diagnostic system.

Research Products

• [Journal Article] 口腔感染制御からSoLAを目指す！ (2013)
  • Author(s): 大森一弘，前田博史，高柴正悟
  • Journal Title: 日本歯科評論 Volume: 73 Pages: 9-11
• [Journal Article] Relationship between periodontitis-related antibody and frequent exacerbations in chronic obstructive pulmonary disease. (2012)
  • Author(s): Takahashi T, Muro S, Tanabe N, Terada K, Kiyokawa H, Sato S, Hoshino Y, Ogawa E, Uno K, Naruishi K, Takashiba S, Mishima M.
  • Journal Title: PLoS One Volume: 7 Issue: 7 Pages: 1-7
  • DOI: 10.1371/journal.pone.0040570
  • NAID: 120004770247
  • Peer Reviewed
• [Journal Article] Assessment of the plasma/serum IgG test to screen for periodontitis. (2012)
  • Author(s): Kudo C
  • Journal Title: J Dent Res Volume: 91 Issue: 12 Pages: 1190-1195
  • DOI: 10.1177/0022034512461796
  • Peer Reviewed
• [Journal Article] Prognosis of periodontitis recurrence after intensive periodontal treatment using examination of serum IgG antibody titer against periodontal bacteria. (2011)
  • Author(s): Sugi N, Naruishi K, Kudo C, Hisaeda-Kako A, Kono T, Maeda H, Takashiba S.
  • Journal Title: J Clin Lab Anal. Volume: 25 Pages: 25-32
  • Peer Reviewed
• [Presentation] 歯周病原細菌の血漿IgG抗体価測定の高速自動化 (2012)
  • Author(s): 山口知子,野添幹雄,工藤値英子,平井公人,江口 徹,前田博史,高柴正悟
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪
• [Presentation] 歯周病原細菌の血漿IgG抗体価測定の高速自動化 (2012)
  • Author(s): 山口知子，野添幹雄，工藤値英子，平井公人，江口徹，前田博史，高柴正悟
  • Organizer: 第22回 日本歯科医学会総会
  • Place of Presentation: 大阪
• [Presentation] IgG 抗体価による歯周病細菌感染度と骨吸収率および高感度 CRP 値との関係 (2012)
  • Author(s): 伊東昌洋，工藤値英子，高柴正悟
  • Organizer: 第22回 日本歯科医学会総会
  • Place of Presentation: 大阪
• [Presentation] 臨床医家と臨床歯科医の連携による歯周病と動脈硬化性疾患の関連性に関する統計学的検討 (2011)
  • Author(s): 工藤値英子, ら
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 下関市
  • Year and Date: 2011-09-24
• [Presentation] 血中IgG抗体価検査用のPorphyromonas gingivalis合成抗原タンパク質の選抜・合成および患者血清との反応性 (2011)
  • Author(s): 山口知子,野添幹雄,工藤値英子,山部こころ,平井公人,江口徹,前田博史,高柴正悟
  • Organizer: 第4回日本口腔検査学会総会・学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-08-27
• [Presentation] 血中IgG抗体価検査用のPorphyromonas gingivalis合成抗原タンパク質の患者血清との反応性 (2011)
  • Author(s): 山口知子,野添幹雄,工藤値英子,山部こころ,平井公人,江口徹,前田博史,高柴正悟
  • Organizer: 第54回春期日本歯周病学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-05-27
• [Presentation] 血中IgG抗体価測定に用いるPorphyromonas gingivalis抗原タンパク質の選抜と合成 (2011)
  • Author(s): 野添幹雄,山口知子,工藤値英子,山部こころ,平井公人,江口 徹,前田博史,高柴正悟
  • Organizer: 第54回春期日本歯周病学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-05-27
• [Presentation] Setting Cut-off Value of Blood Test for Periodontitis and Pneumonia (2011)
  • Author(s): S.TAKASHIBA, C.KUDO, K.NARUISHI, H.MAEDA
  • Organizer: 2011 IADR GEneral Session
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-18
• [Presentation] Discrepancy between IgG Titer and Clinical Indexes in Chronic Periodontitis (2011)
  • Author(s): M.ITO, S.KOCHI, C.KUDO, S.TAKASHIBA
  • Organizer: 2011 IADR GEneral Session
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-18
• [Presentation] 血中IgG抗体価測定に用いるPorphyromonas gingivalis抗原タンパク質の選抜と合成 (2011)
  • Author(s): 野添幹雄, ら
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡市
• [Presentation] 血中IgG抗体価検査用のPorphyromonas gingivalis合成抗原タンパク質の患者血清との反応性 (2011)
  • Author(s): 山口知子, ら
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡市
• [Presentation] Evaluation of Periodontitis by a Blood Test (2010)
  • Author(s): S.TAKASHIBA, C.KUDO, K.NARUISHI, H.MAEDA
  • Organizer: 2010 IADR GEneral Session
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-17
• [Presentation] 口腔から全身が見える：よく分かる歯周病菌抗体法
  • Author(s): 高柴正悟
  • Organizer: 第22回 日本歯科医学会総会
  • Place of Presentation: 大阪
• [Patent(Industrial Property Rights)] 歯周病原菌血漿または血清抗体価検査キット (2011)
  • Inventor(s): 野添幹雄
  • Industrial Property Rights Holder: サンスター株式会社
  • Industrial Property Number: 2011-228984
  • Filing Date: 2011-10-18
• [Patent(Industrial Property Rights)] 歯周病原菌血漿または血清抗体価検査キット (2011)
  • Inventor(s): 野添幹雄
  • Industrial Property Rights Holder: サンスター株式会社
  • Filing Date: 2011-10-18
  • Overseas
• [Patent(Industrial Property Rights)] 歯周病原菌血漿または血清抗体価検査キット (2010)
  • Inventor(s): 野添幹雄
  • Industrial Property Rights Holder: サンスター株式会社
  • Industrial Property Number: 2010-279268
  • Filing Date: 2010-12-15