| Project/Area Number |
22405014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Japan Women's University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NOZAKI Hisayoshi 東京大学, 大学院理学系研究科, 准教授 (40250104)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUCHIKANE Yuki 日本女子大学, 理学部, 助教 (20434152)
ABE Jun 日本女子大学, 理学部, 学術研究員 (10424764)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | ミカヅキモ / 生殖隔離 / 性フェロモン / 種分化 / 有性生殖 / ホモタリズム / ヘテロタリズム |
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| Research Abstract |
The Closterium peracerosum-strigosum-littorale complex (C. p-s-l. complex) is a unicellular alga, and is known to be composed of several reproductively isolated mating groups of heterothallic strains. We carried out field studies in foreign countries and in Japan, to isolate reproductively isolated C. p-s-l. complex. Finally, we established several unialgal clonal cultures from these fields.
We studied phylogenetic relationship using DNA sequences of 1506 group I introns of these clones. Based on the alignment, significant phylogenetic relationships were observed between newly established mating group G and mating group I-E. Because genome project of cells belonging to the group I-E is ongoing, newly established group G would be suitable for the understanding of the mechanism of reproductive isolation, in the molecular biological aspect.
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