Development of an assay to evaluate the reactivity at chemicals contaminated in the atmosphere, South California
| Project/Area Number |
22406004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Environmental pharmacy
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHINKAI Yasuhiro 筑波大学, 医学医療系, 助教 (10454240)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 大気中揮発物質 / 親電子物質 / Keap1/Nrf2 / グルタチオン / 化学修飾 / Nrf2/Keap1システム |
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| Research Abstract |
We describe here results of studies investigating the actions of the vapor phase of ambient air samples collected in the midtown area of Los Angeles on human bronchial epithelial BEAS-2B cells using DNA microarray analysis. Among 26 genes whose expression increased 4 fold or more, 4 genes were associated with detoxifying genes regulated by the transcription factor Nrf2. Consistent with these results, the vapor samples activate the Nrf2-ARE pathway, resulting in up-regulation of heme oxygenase-1 (HO-1), glutamate cysteine ligase modifier subunit, and cystine transporter (xCT) mRNA and proteins. No appreciable increases in pro-inflammatory genes were observed. These results suggest that ambient vapor samples activate the Nrf2-ARE pathway but not an inflammatory response.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Initial response and cellular protection through the Keap1/Nrf2 system during exposure of primary mouse hepatocytes to 1,2-naphthoquinone2011
Author(s)
Miura T, Shinkai Y, Jiang HY, Iwamoto N, Sumi D, Taguchi K, Yamamoto M, Jinno H, Tanaka-Kagawa T, Cho AK, Kumagai Y
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Journal Title
Chem Res Toxicol
Volume: 24
Issue: 4
Pages: 559-567
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of sensory nerves and TRPV1 receptors in the rat airway inflammatory response to two environment pollutants: diesel exhaust particles (DEP) and 1,2-naphthoquinone (1,2-NQ)2010
Author(s)
Teles AM, Kumagai Y, Brain SD, Teixeira SA, Varriano AA, Barreto MA, de Lima WT, Antunes E, Muscara MN, Costa SK.
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Journal Title
Archives of Toxicology
Volume: 84
Issue: 2
Pages: 109-117
DOI
Related Report
Peer Reviewed
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