Immunogenetic analysis of early onset schistosomal fibrosis in the Philippines

• Project/Area Number: 22406009
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: KIKUCHI Mihoko 長崎大学, 熱帯医学研究所, 講師 (40336186)
• Co-Investigator(Kenkyū-buntansha): CHIGUSA Yuichi 獨協医科大学, 医学部, 教授 (20171936) ; HIRAYAMA Kenji 長崎大学, 熱帯医学研究所, 教授 (60189868)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 日本住血吸虫 / フィリピン / 肝線維化症 / 免疫応答性 / 遺伝解析 / HLA-DRB1*1501 / mRNA / 若年性肝線維化症 / 免疫応答 / 国際研究者交流 / 若年性 / 住血吸虫中卵抗原 / 宿主遺伝要因 / 組織適合抗原(HLA)

Research Abstract

Post-schistosomal hepatic fibrosis and cirrhosis are the major sequelae resulting from excessive immune response to deposited eggs in the portal area. In humans little is known about immunoregulatory mechanisms controlling the development of hepatosplenic disease. The objective of the study is to determine the prevalence of the early onset schistosomal fibrosis and the different host genetic factors and mechanism behind the immune responses in patients with early onset schistosomal liver fibrosis in the Sorsogon Province, Philippines. The subjects were subjected to Kato-katz stool examination, ultrasonographic examination, and detection of anti-SEA antibody, located in the schistosomiasis endemic barangays of the municipality of Irosin, in the province of Sorsogon. Results of imuunogenetical analysis showed measure immune-responses in early onset post schistosomal fibrosis was restricted HLA-DRB1*15:01 and progression was related on expression level of HLA-DRB1 gene.

Research Products

• [Journal Article] Detection of active schistosome infection by cell-free circulating DNA of Schistosoma japonicum in highly endemic areas in Sorsogon Province, the Philippines (2014)
  • Author(s): Kato-Hayashi N, Leonardo LR, Arevalo NL, Tagum MN, Apin J, Agsolid LM, Chua JC, Villacorte EA, Kirinoki M, Kikuchi M, Ohmae H, Haruki K, Chigusa Y
  • Journal Title: Acta Trop Volume: (pii : S0001-706X(14)00168-5)
• [Journal Article] Characterization of a gene family encoding SEA (sea-urchin sperm protein, enterokinase and agrin)-domain proteins with lectin-like and heme-binding properties from Schistosoma japonicum (2014)
  • Author(s): Mbanefo EC, Kikuchi M, Huy NT, Shuaibu MN, Cherif MS, Yu C, Wakao M, Suda Y, Hirayama K
  • Journal Title: PLoS Negl Trop Dis Volume: 8(1) Issue: 1 Pages: 2644-2644
  • DOI: 10.1371/journal.pntd.0002644
  • NAID: 120006986695
  • Peer Reviewed
• [Journal Article] Genetic Structure Inferred from Mitochondrial 12S Ribosomal RNA Sequence of Oncomelaniaquadrasi, the Intermediate Snail Host of Schistosoma japonicum in the Philippines (2014)
  • Author(s): Weerachai Saijuntha, Blanca Jarilla, Alvin K. Leonardo, Louie S. Sunico, Lydia R. Leonardo, Ross H. Andrews, Paiboon Sithithaworn, Trevor N. Petney, Masashi Kirinoki, Naoko Kato-Hayashi, Mihoko Kikuchi, Yuichi Chigusa, and Takeshi Agatsuma
  • Journal Title: American Jornal of Tropical Medicine and Hygene Volume: (Epub ahead of print) Issue: 6 Pages: 1-12
  • DOI: 10.4269/ajtmh.13-0260
  • Peer Reviewed
• [Journal Article] New endemic foci of schistosomiasis infections in the Philippines. (2014)
  • Author(s): Leonardo L
  • Journal Title: Ａｃｔａ ｔｒｏｐ Volume: in press
  • Peer Reviewed
• [Journal Article] Use of cell-free circulating schistosome DNA in serum, urine, semen, and saliva to monitor a case of refractory imported schistosomiasis hematobia (2013)
  • Author(s): Kato-Hayashi N, Yasuda M, Yuasa J, Isaka S, Haruki K, Ohmae H, Osada Y, Kanazawa T, Chigusa Y
  • Journal Title: J Clin Microbiol Volume: 51(10) Issue: 10 Pages: 3435-8
  • DOI: 10.1128/jcm.01219-13
  • Peer Reviewed
• [Journal Article] フィリピンにおける日本住血吸虫症有病地の現状 (2013)
  • Author(s): 林尚子、菊池三穂子、千種雄一
  • Journal Title: 日本獣医寄生虫学雑誌JPN.J. Vet. Parasitol Volume: Vol.12 (2) Pages: 79-86
  • URL: http://jsvp.umin.jp/index.html
• [Journal Article] 21-Hydroxylase gene mutant allele CYP21A2*15 strongly linked to the resistant HLA Haplotype B*14:02-DRB1*01:02 in Chronic Chagas Disease (2013)
  • Author(s): Puerto FD
  • Journal Title: Hum Immunol Volume: 74(6) Pages: 783-6
  • Peer Reviewed
• [Journal Article] Origin of a novel protein-coding gene family with similar signal sequence in Schistosoma japonicum (2012)
  • Author(s): Mbanefo EC, Chuanxin Y, Kikuchi M, Shuaibu MN, Boamah D, Kirinoki M, Hayashi N, Chigusa Y, Osada Y, Hamano S, Hirayama K
  • Journal Title: BMC Genomics Volume: 13 Issue: 1 Pages: 260-260
  • DOI: 10.1186/1471-2164-13-260
  • NAID: 120006986371
  • Peer Reviewed
• [Journal Article] Immunoproteomics Identification of Major IgE and IgG4 Reactive Schistosoma japonicum Adult Worm Antigens Using Chronically Infected Human Plasma. (2012)
  • Author(s): Boamah D,
  • Journal Title: Trop Med Health. Volume: 40(3) Pages: 89-102
  • Peer Reviewed
• [Journal Article] Association of HLA and post-schistosomal hepatic disorder : A systematic review and meta-analysis (2011)
  • Author(s): Huy NT, Hamada M, Kikuchi M, Lan NT, Yasunami M, Zamora J, Hirayama K
  • Journal Title: Parasitol Int Volume: 60(4) Pages: 347-356
  • NAID: 120006985063
  • Peer Reviewed
• [Presentation] Surveyllance on Schistosomal Fibrosis in Sorsogon Province, the Phillippins (2013)
  • Author(s): 菊池三穂子他
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 東京東京医科歯科大学御茶ノ水キャンパス
• [Presentation] Surveyllance on Schistosomal Fibrosis in Sorsogon Province, the Phillippins (2013)
  • Author(s): 菊池三穂子
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学 御茶ノ水キャンパス、東京都
• [Presentation] Immunogenetic analysis of the patients with early onset schistosomal fibrosis in Sorsogon Province, the Philippines (2012)
  • Author(s): Kikuchi M, Leonardo LR, Chigusa Y, Segubre-Mercado EM, Kobayashi N, Hayashi N, Inoue T, Arevalo NL, Lim RR, Agsolid LM, Agatsuma K, Hirayama K
  • Organizer: 45th Annual Japan-U.S.Joint Conference on Parasitic Diseases, Japan-US Parasitic Diseases Panel Meeting, Japan-US Cooperative Medical Science Program
  • Place of Presentation: 東京
  • Year and Date: 2012-01-10
• [Presentation] Immunogenetic analysis of the patients with early-onset of schistosomal liver fibrosis in Sorsogon Province, the Philippines (2012)
  • Author(s): Kikuchi. M. et al
  • Organizer: 5th The ASEAN Congress of Tropical Medicine and Parasitology (ACTMP)
  • Place of Presentation: Manila, Philippine, University of Philippine
• [Presentation] Immunogenetic analysis of the patients with early-onset of schistosomal liver fibrosis in Sorsogon Province, the Philippines (2012)
  • Author(s): Kikuchi. M. et al
  • Organizer: Forum Cheju 15
  • Place of Presentation: 宮崎宮崎パームビーチホテル
• [Presentation] Origin of novel protein-coding genes with similar signal sequence in Schistosoma japonicum (2012)
  • Author(s): Evaristus CM, Kikuchi M., et al
  • Organizer: 日本熱帯医学会
  • Place of Presentation: 北海道とかちプラザ
• [Presentation] Surveyllance on Schistosomal Fibrosis in Sorsogon Province, the Phillippins (2012)
  • Author(s): 菊池三穂子他
  • Organizer: 日本熱帯医学会
  • Place of Presentation: 北海道とかちプラザ
• [Presentation] Immunogenetic analysis of the patients with early-onset of schistosomal liver fibrosis in Sorsogon Province, the Philippines. (2012)
  • Author(s): Kikuchi. M
  • Organizer: 5th The ASEAN Congress of Tropical Medicine and Parasitology (ACTMP)
  • Place of Presentation: University of the philippines, Manila, Philippine
• [Presentation] Immunegenetic analysis of patients with early onset schistosomal fibrosis in Sorsogon Province, the Philippines. (2012)
  • Author(s): 菊池三穂子
  • Organizer: Forum Cheju 15
  • Place of Presentation: 宮崎パームビーチホテル, 宮崎市
• [Presentation] Origin of novel protein-coding genes with similar signal sequence in Schistosoma japonicum (2012)
  • Author(s): Evaristus CＭ
  • Organizer: 日本熱帯医学会
  • Place of Presentation: とかちプラザ、帯広市、北海道
• [Presentation] Surveyllance on Schistosomal Fibrosis in Sorsogon Province, the Phillippins (2012)
  • Author(s): 菊池三穂子
  • Organizer: 日本熱帯医学会
  • Place of Presentation: とかちプラザ、帯広市、北海道
• [Presentation] Immunogenetic analysis of the patients with early onset schistosomal fibrosis in Sorsogon Province, the Philippines (2011)
  • Author(s): Kikuchi M, et al
  • Organizer: 45th Annual Japan-U.S. Joint Conference on Parasitic Diseases, Japan-US Parasitic Diseases Panel Meeting, Japan-US Cooperative Medical Science Program
  • Place of Presentation: 東京、東京大学
• [Presentation] フィリピンにおける若年性住血吸虫性肝線維化症の発症に関わる免疫関連遺伝 (2010)
  • Author(s): 菊池三穂子
  • Organizer: 第51回熱帯医学会大会
  • Place of Presentation: 仙台市
• [Book] 感染症事典 (2012)
  • Author(s): 感染症事典編集委員会編共著菊池三穂子
  • Total Pages: 617
  • Publisher: オーム社
• [Book] 感染症事典 (2012)
  • Author(s): 感染症事典編集委員会 編 共著 菊池三穂子
  • Publisher: オーム社