|Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Safe and efficacious vaccines have been introduced to protect children from rotavirus, a major cause of severe acute gastroenteritis in children worldwide. This study aimed at providing a molecular epidemiologic basis on which to understand the effects of rotavirus vaccines on circulating wild-type rotavirus strains by analyzing clinical samples collected from children admitted to a sentinel hospital in Blantyre, Malawi. This study revealed that Malawian G8 strains evolved over the last decade through genetic reassortment with other human rotaviruses, changing their VP4 genotypes while maintaining conserved genotype constellations for the remaining structural and non-structural proteins. Analysis of Malawian G12P strains and representative, co-circulating strains provided molecular evidence for complex stages of reassortment events that a G12 VP7 gene donor strain underwent with contemporary, locally-circulating strains possessing different genetic backgrounds.