Studies on motor axon guidance in a spontaneous mutant mouse

• Project/Area Number: 22500284
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: University of Tsukuba
• Principal Investigator: KEINO-MASU Kazuko 筑波大学, 医学医療系, 講師 (50344883)
• Co-Investigator(Kenkyū-buntansha): MASU Masayuki 筑波大学, 医学医療系, 教授 (20243032)
• Co-Investigator(Renkei-kenkyūsha): ARINAMI Tadao 筑波大学, 医学医療系, 教授 (10212648)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 脳・神経 / 遺伝子 / 発生・分化 / 神経科学

Research Abstract

The molecular mechanisms that regulate guidance of spinal motor axons to their target muscles are not known. In this study, we tried to elucidate it using a spontaneous mutant mouse that shows abnormalities in a particular motor trajectory. We examined the sequences of the genes in the critical region but failed to find any mutation responsible for the mutant phenotype. However, we found that the expression levels of several genes present in the critical region are changed in the mutant mice, suggesting the abnormalities in gene regulation.

Research Products

• [Journal Article] ヘパラン硫酸微細構造の組織特異性とその決定機構 (2013)
  • Author(s): 桝和子、桝正幸
  • Journal Title: 医学のあゆみ Volume: 245 (7) Pages: 600-602
  • Peer Reviewed
• [Journal Article] ErbB2 Dephosphorylation and Anti-Proliferative Effects of Neuregulin-1 in ErbB2-Overexpressing Cell Lines (2013)
  • Author(s): Wang, R., Iwakura, Y., Araki, K., Keino-Masu, K., Masu, M., Wang, X., Takei, N., Higashiyama, S. and Nawa, H.
  • Journal Title: Potential Contribution of Their Low-Affinity Interaction. Scientific reports Volume: 3 Pages: 1402-1402
  • Peer Reviewed
• [Journal Article] ErbB2 dephosphorylation and anti-proliferative effects of neuregulin-1 in ErbB2-overexpressing cell lines; re-evaluation of their low-affinity interaction. (2013)
  • Author(s): Wang, R., Iwakura, Y., Araki, K., Keino-Masu, K., Masu, M., Wang, X., Takei, N., Higashiyama, S. and Nawa, H.
  • Journal Title: Scientific reports Volume: 3 Issue: 1 Pages: 1402-1402
  • DOI: 10.1038/srep01402
  • Peer Reviewed
• [Journal Article] Organ-Specific Sulfation Patterns of Heparan Sulfate Generated by Extracellular Sulfatases Sulf1 and Sulf2 in Mice (2012)
  • Author(s): Nagamine, S., Tamba, M., Ishimine, H., Araki, K., Shiomi, K., Okada, T., Ohto, T., Kunita, S., Takahashi, S., Wismans, R.G.P., van Kuppevelt, T.H., Masu, M., and Keino-Masu, K
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 9579-9590
  • NAID: 120007130630
  • Peer Reviewed
• [Journal Article] Organ-Specific Sulfation Patterns of Heparan Sulfate Generated by Extracellular Sulfatases Sulf1 and Sulf2 in Mice. (2012)
  • Author(s): Nagamine, S., Tamba, M., Ishimine, H., Araki, K., Shiomi, K., Okada, T., Ohto, T., Kunita, S., Takahashi, S., Wismans, R.G.P., van Kuppevelt, T.H., Masu, M., and Keino-Masu, K.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 12 Pages: 9579-9590
  • DOI: 10.1074/jbc.m111.290262
  • NAID: 120007130630
  • Peer Reviewed
• [Journal Article] Crystallographic characterization of the DIX domain of the Wnt signalling positive regulator Ccd1 (2011)
  • Author(s): Terawaki, S., Yano, K., Katsutani, T., Shiomi, K., Keino-Masu, K., Masu, M., Shomura, Y., Komori, H., Shibata, N., and Higuchi, Y
  • Journal Title: Acta Crystallographica Volume: F67 Pages: 758-761
  • Peer Reviewed
• [Journal Article] Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development. (2011)
  • Author(s): Koike, S., Yutoh, Y., Keino-Masu, K., Noji, S., Masu, M., and Ohuchi, H
  • Journal Title: Developmental Dynamics Volume: 240 Pages: 413-421
  • Peer Reviewed
• [Journal Article] Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development (2011)
  • Author(s): Koike, S., Yutoh, Y, Keino-Masu, K., Noji, S., Masu, M., Ohuchi, H.
  • Journal Title: Developmental Dynamics Volume: 240 Pages: 413-421
  • Peer Reviewed
• [Journal Article] Crystallographic characterization of the DIX domain of the Wnt signalling positive regulator Ccd1 (2011)
  • Author(s): Terawaki, S., Yano, K, Katsutani, T., Shiomi, K., Keino-Masu, K., Masu, M., Shomura, Y., Komori, H., Shibata, N., Higuchi, Y.
  • Journal Title: Acta Crystallographica Section F Volume: 67 Pages: 758-761
  • Peer Reviewed
• [Journal Article] Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development. (2011)
  • Author(s): Koike, S., Yutoh, Y., Keino-Masu, K., Noji, S., Masu, M., Ohuchi, H.
  • Journal Title: Dev.Dyn. Volume: 240 Pages: 413-421
  • Peer Reviewed
• [Journal Article] Association of the HSPG2 Gene with Neuroleptic-Induced Tardive Dyskinesia (2010)
  • Author(s): Syu, A., Ishiguro, H., Inada, T., Horiuchi, Y., Tanaka, S., Ishikawa, M., Arai, M., Itokawa, M., Niizato, K., Iritani, S., Ozaki, N., Takahashi, M., Kakita, A., Takahashi, H., Nawa, H., Keino-Masu, K., Arikawa-Hirasawa, E., and Arinami, T.
  • Journal Title: Neuropsychopharmacology Volume: 35 Pages: 1155-1164
  • Peer Reviewed
• [Journal Article] Combining the Cell Encapsulation Technique and Axon Guidance for Cell Transplantation Therapy (2010)
  • Author(s): Yamazoe, H., Keino-Masu, K., and Masu, M
  • Journal Title: Journal Biomaterial Science Volume: 21 Pages: 1815-1826
  • NAID: 120007137895
  • Peer Reviewed
• [Journal Article] Deficiency of autotaxin/lysophospholipase D results in head cavity formation in mouse embryos through the LPA receptor-Rho-ROCK pathway (2010)
  • Author(s): Koike, S., Keino-Masu, K., and Masu, M
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 400 Pages: 66-71
  • NAID: 120007137917
  • Peer Reviewed
• [Journal Article] Proteolytic cleavage of the rat heparan sulfate 6-O-endosulfatase SulfFP2 by furin-type proprotein convertases. (2010)
  • Author(s): Nagamine, S., Keino-Masu, K., Shiomi, K., and Masu, M
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 391 Pages: 107-112
  • Peer Reviewed
• [Journal Article] Proteolytic cleavage of the rat heparan sulfate 6-O-endosulfatase SulfFP2 by furin-type proprotein convertases. (2010)
  • Author(s): Nagamine, S., Keino-Masu, K.,Shiomi, K., Masu, M
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 391 Pages: 107-112
  • Peer Reviewed
• [Journal Article] Deficiency of autotaxin/lysophospholipase D results in head cavity formation in mouse embryos through the LPA receptor-Rho-ROCK pathway. (2010)
  • Author(s): Koike, S., Keino-Masu, K., Masu, M.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 400 Pages: 66-71
  • NAID: 120007137917
  • Peer Reviewed
• [Journal Article] Combining the Cell Encapsulation Technique and Axon Guidance for Cell Transplantation Therapy. (2010)
  • Author(s): Yamazoe, H., Keino-Masu, K., Masu, M.
  • Journal Title: J.Biomaterial Sci. Volume: 21 Pages: 1815-1826
  • NAID: 120007137895
  • Peer Reviewed
• [Journal Article] Association of the HSPG2 Gene with Neuroleptic-Induced Tardive Dyskinesia. (2010)
  • Author(s): Syu, A., Ishiguro, H., Inada, T., Horiuchi, Y., Tanaka, S., Ishikawa, M., Arai, M., Itokawa, M., Niizato, K., Iritani, S., Ozaki, NTakahashi, M., Kakita, A., Takahashi, H., Nawa, H., Keino-Masu, K., Arikawa-Hirasawa, E., Arinami, T.
  • Journal Title: Neuropsychopharmacology Volume: 35 Pages: 1155-1164
  • Peer Reviewed
• [Presentation] Role of podocyte extracellular sulfatase in regulation of glomerular permeability. (2012)
  • Author(s): Takashima, Y., Yamashita, H., Sakamoto, K., Hara, S., Kobayashi, N., Ueno, T., Keino-Masu, K., Masu, M., and Nagata, M.
  • Organizer: Kideney week 2012
  • Place of Presentation: San Diego Convention Center , San Diego, 米国
• [Presentation] Heparan Sulfate Modification by Endosulfatases is Required for Cortical Axon Guidance (2012)
  • Author(s): Masu, M., Okada, T., Nagamine, S., Ohto, T., Kametani, F., Hasegawa, M., Kunita, S., Takahashi, S., and Keino-Masu, K
  • Organizer: 1^<st>international symposium/ 59^<th>NIBB conferences "Neocortical organization"
  • Place of Presentation: 岡崎カンファレンスセンター、岡崎
• [Presentation] Role of podocyte extracellular sulfatase in regulation of glomerular permeability. (2012)
  • Author(s): Takashima, Y., Yamashita, H., Sakamoto, K., Hara, S., Kobayashi, N., Ueno, T., Keino-Masu, K., Masu, M. and Nagata, M.
  • Organizer: Kidney week 2012
  • Place of Presentation: San Diego Convention Center（米国）
• [Presentation] Heparan Sulfate Modification by Endosulfatases is Required for Cortical Axon Guidance (2012)
  • Author(s): Masayuki Masu, Takuya Okada, Satoshi Nagamine, Tatsuyuki Ohto, Fuyuki Kametani, Masato Hasegawa, Satoshi Kunita, Satoru Takahashi, Kazuko Keino-Masu
  • Organizer: 1^<st> international symposium/59^<th>NIBB conferences "Neocortical organization"
  • Place of Presentation: 岡崎カンファレンスセンター(岡崎)(招待講演)
• [Presentation] Roles of heparan sulfate endosulfatases in neural development (2011)
  • Author(s): Masu, M.
  • Organizer: The 3rd Workshop between University of Tsukuba and University of Edinburgh
  • Place of Presentation: University of Edinburgh, Edinburgh, 英国
  • Year and Date: 2011-06-13
• [Presentation] ヘパラン硫酸糖鎖による軸索ガイダンス制御機構 (2011)
  • Author(s): 桝正幸、岡田拓也、長嶺聖史、大戸達之、亀谷富由樹、長谷川成人、國田智、高橋智、桝和子
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: パシフィコ横浜、横浜
• [Presentation] ヘパラン硫酸糖鎖による軸索ガイダンス制御機構 (2011)
  • Author(s): 桝正幸、岡田拓也、長嶺聖史、大戸達之、亀谷富由樹、長谷川成人、國田智、高橋智、桝和子
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: パシフィコ横浜(横浜)(招待講演)
• [Presentation] The roles of heparan sulfate in axon guidance (2010)
  • Author(s): 桝正幸
  • Organizer: International Graduate Schools International Conference
  • Place of Presentation: つくば国際会議場、つくば
  • Year and Date: 2010-11-01
• [Presentation] Autotaxin is required for anterior neural ridge formation, midbrain-hindbrain boundary patterning, and cranial neural tube closure. (2010)
  • Author(s): Tagiuchi, E., Koike, S., Yutoh, Y. ,Keino-Masu, K., Noji, S., Masu, M., Ohuchi, H
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] Wntシグナル伝達で機能するCCD1 DIXドメインのX線結晶構造解析 (2010)
  • Author(s): 寺脇慎一、塩見健輔、桝正幸、柴田直樹、樋口芳樹
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] Heparan sulfate 6-Oendosulfatases are required for neural network formation. (2010)
  • Author(s): Okada, T., Keino-Masu, K., Nagamine, S., Kunita, S., Takahashi, S., and Masu, M
  • Organizer: The 40th Annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, convenction center, San Diego,米国
• [Presentation] Wntシグナル伝達で機能するCCD1オリゴマー形成機構に関する構造学的研究 (2010)
  • Author(s): 寺脇慎一、塩見健輔、桝正幸、柴田直樹、樋口芳樹
  • Organizer: 日本結晶学会2010年年会
  • Place of Presentation: 大阪大学コンベンションセンター、大阪
• [Presentation] マウス胎児脳におけるヘパラン硫酸多様性の検出 (2010)
  • Author(s): 岡田拓也、桝和子、長嶺聖史、國田智、高橋智、Arie Oosterhof, Toin Kuppevelt、桝正幸
  • Organizer: 第33回日本神経科学学会大会・第53回日本神経化学会大会・第20回日本神経回路学会大会
  • Place of Presentation: 神戸コンベンションセンター、神戸
• [Presentation] Wntシグナル伝達機構ではたらくCCD1の構造化学的研究 (2010)
  • Author(s): 寺脇慎一、塩見健輔、桝正幸、柴田直樹、樋口芳樹
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
• [Presentation] マウス胎児脳におけるヘパラン硫酸多様性の検出 (2010)
  • Author(s): 岡田拓也、桝和子、長嶺聖史、國田智、高橋智、Arie Oosterhof, Toin Kuppevelt、桝正幸
  • Organizer: 第33回日本神経科学学会大会・第53回日本神経化学会大会・第20回日本神経回路学会大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫)
• [Presentation] Heparan sulfate 6-Oendosulfatases are required for neural network formation. (2010)
  • Author(s): T.Okada, K.Keino-Masu, S.Nagamine, S.Kunita, S.Takahashi, M.Masu
  • Organizer: The 40th Annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, convenction center(米国)
• [Presentation] Autotaxin is required for anterior neural ridge formation, midbrain-hindbrain boun dary patteming, and cranial neural tube closure. (2010)
  • Author(s): E.Tagiuchi, S.Koike, Y.Yutoh, K.Keino-Masu, S.Noji, M.Masu, H.Ohuchi.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場(兵庫)
• [Book] Lysophospholipid Receptors: Signaling and Biochemistry (2013)
  • Author(s): Masu, M., Koike, S., Okada, T., and Keino-Masu, K.
  • Publisher: John Wiley & Son Inc.
• [Book] Studies on autotaxin signaling in endocytic vesicle biogenesis and embryonic development using whole embryo culture and electroporation. In: Lysophospholipid Receptors
  • Author(s): Masu, M., Koike, S., Okada, T., and Keino-Masu, K
  • Publisher: Signaling and Biochemistry.
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/duo/molneurobiol/
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/molneurobiol/index.html