Studies on the reguratory mechanisms for maintainenance and differentiation of neural stem cells in the developmental brain

• Project/Area Number: 22500289
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Nagoya University
• Principal Investigator: KAWAGUCHI Ayano 名古屋大学, 医学系研究科, 准教授 (90360528)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 発生 / 発達 / 再生神経科学 / 神経科学 / 神経発生 / 神経幹細胞 / 単一細胞cDNA / 大脳発生 / 神経分化 / in vivoエレクトロポレーション

Research Abstract

Genome-wide transcriptome profiles of single mouse cortical progenitors identified a set of temporally-regulated genes in embryonic neural stem cells (NSCs). Early NSCs showed cell-to-cell variations of Notch signaling-related gene expression, while later NSDs were more homogenous in Notch signaling status, suggesting a shift of NSC-maintaining strategy. I also found that neither initial oscillatory Notch activation nor cell cycle progression is necessary for NSCs’ internal “clock” mechanisms.

Research Products

• [Journal Article] Lunatic fringe potentiates Notch signaling in the developing brain. (2010)
  • Author(s): Kato TM, Kawaguchi A, Kosodo Y, Niwa H, Matsuzaki F
  • Journal Title: Mol Cell Neurosci Volume: 45 Pages: 12-25
  • Peer Reviewed
• [Journal Article] Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum. (2010)
  • Author(s): Miyata M, Ono Y, Okamoto M, Masaoka M, Sakakibara A, Kawaguchi A, Hashimoto M, Ogawa M.
  • Journal Title: Neural Development Volume: 5 Issue: 1 Pages: 23-23
  • DOI: 10.1186/1749-8104-5-23
  • Peer Reviewed
• [Presentation] 大脳発生期における神経前駆細胞の単一細胞遺伝子発現プロファイリング (2012)
  • Author(s): 川口綾乃
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2012-09-19
• [Presentation] 大脳皮質発生過程における神経前駆細胞の遺伝子発現変化 (2012)
  • Author(s): 川口綾乃
  • Organizer: 平成 24 年度 包括型脳科学研究推進支援ネットワーク
  • Place of Presentation: 仙台国際センター、仙台
  • Year and Date: 2012-07-26
• [Presentation] Temporal Change of Gene Expression Pattern in Neural Progenitor Cells during Neocortical Development (2012)
  • Author(s): 川口綾乃
  • Organizer: 第一回国際シンポジウム"Neocortical Organization
  • Place of Presentation: 岡崎コンファレンスセンター、岡崎
  • Year and Date: 2012-03-12
• [Presentation] Temporal Change of Gene Expression Pattern in Neural Progenitor Cells during Neocortical Development (2012)
  • Author(s): 川口綾乃
  • Organizer: 第一回国際シンポジウム"Neocortical Organization"
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2012-03-12
• [Presentation] 大脳発生過程における前駆細胞の遺伝子発現の変化 (2011)
  • Author(s): 川口綾乃
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2011-09-17
• [Presentation] 大脳発生過程における前駆細胞の遺伝子発現の変化 (2011)
  • Author(s): 川口綾乃
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-17
• [Presentation] Characterization of the neocortical progenitors during development as revealed by single-cell gene expression profile (2010)
  • Author(s): 川口綾乃
  • Organizer: 新学術領域「動く細胞と秩序」第2回班会議
  • Place of Presentation: ロイヤルパークイン名古屋、名古屋
  • Year and Date: 2010-12-18
• [Presentation] Characterization of the neocortical progenitors during developmentas revealed by single-cell gene expression profiles. (2010)
  • Author(s): 川口綾乃
  • Organizer: 新学術領域「動く細胞と秩序」第2回班会議
  • Place of Presentation: ロイヤルパークイン名古屋
  • Year and Date: 2010-12-18
• [Presentation] 大脳皮質発生過程における神経前駆細胞の遺伝子発現変化
  • Author(s): 川口綾乃
  • Organizer: 平成24年度 包括型脳科学研究推進支援ネットワーク
  • Place of Presentation: 仙台
• [Presentation] 大脳発生期における神経前駆細胞の単一細胞遺伝子発現プロファイリング
  • Author(s): 川口綾乃
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋