Novel transcriptional repression mechanisms that negatively regulate neuronal differentiation

• Project/Area Number: 22500292
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Osaka University
• Principal Investigator: KAMACHI Yusuke 大阪大学, 生命機能研究科, 准教授 (90263334)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 遺伝子 / 発生・分化 / ゲノム

Research Abstract

Mechanisms of how neural stem/precursor cells are established and maintained have been important questions. In this study, we investigated mechanisms of how group B1 Sox transcription factors (Sox1/2/3/19) regulate neural stem cell states using zebrafish as a model system. We show that transcriptional repressors including Zic1 and several miRNA are involved in B1 Sox-dependent-inhibition of neuronal differentiation.

Research Products

• [Journal Article] Sox proteins: regulators of cell fate specification and differentiation (2013)
  • Author(s): Kamachi Y, Kondoh H
  • Journal Title: Development Volume: 140 Issue: 20 Pages: 4129-4144
  • DOI: 10.1242/dev.091793
  • Peer Reviewed
• [Journal Article] Nano-analysis of DNA conformation changes induced by transcription factor complex binding using plasmonic nanodimers (2013)
  • Author(s): Morimura H, Tanaka S, Ishitobi H, Mikami T, Kamachi Y, Kondoh H, Inouye Y.
  • Journal Title: ACS Nano Volume: 7 Issue: 12 Pages: 10733-10740
  • DOI: 10.1021/nn403625s
  • Peer Reviewed
• [Journal Article] An Efficient Binary System for Gene Expression in the Silkworm, Bombyx mori, Using GAL4 Variants (2011)
  • Author(s): Kobayashi I., Sezutsu H. 他10名
  • Journal Title: Archives of Insect Biochemistry & Physiology Volume: 76 Issue: 4 Pages: 195-210
  • DOI: 10.1002/arch.20402
  • Peer Reviewed
• [Journal Article] The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates (2011)
  • Author(s): Iwafuchi-Doi M
  • Journal Title: Developmental Biology Volume: 352 Issue: 2 Pages: 354-366
  • DOI: 10.1016/j.ydbio.2010.12.027
  • Peer Reviewed
• [Journal Article] The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates (2011)
  • Author(s): Iwafuchi-Doi M., Kamachi Y, et al.
  • Journal Title: Developmental Biology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Bl SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryo (2010)
  • Author(s): Okuda, Y., Ogura, E., Kondoh, H. and Kamachi, Y.
  • Journal Title: PLoS Genet Volume: 6(5) Issue: 5 Pages: e1000936-e1000936
  • DOI: 10.1371/journal.pgen.1000936
  • Peer Reviewed
• [Journal Article] B1 SOX Coordinate Cell Specification with Patterning and Morphogenesis in the Early Zebrafish Embryo (2010)
  • Author(s): Okuda Y., Kamachi Y, et al.
  • Journal Title: PLoS Genetics Volume: 6(5)
  • Peer Reviewed
• [Presentation] Soxllに依存したmiRNA-17-92クラスター発現の制御機構 (2013)
  • Author(s): 川合杏奈, 近藤寿人, 蒲池雄介
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] SoxllはmicroRNA遺伝子の転写活性化を通して匪発生を制御する (2012)
  • Author(s): 蒲池雄介, 川合杏奈, 近藤寿人
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Group Bl and C sox genes in zebrafish development (2011)
  • Author(s): Kamachi Y.
  • Organizer: Third International Sox Meeting
  • Place of Presentation: Grainau Germany
  • Year and Date: 2011-09-12
• [Presentation] Group B1 and C sox genes in zebrafish development (2011)
  • Author(s): Kamachi Y.
  • Organizer: Third Sox meeting
  • Place of Presentation: Grainau, Germany(招待講演)
  • Year and Date: 2011-09-12
• [Presentation] Bl SOX Coordinate Cell Specification with Patterning and Morphogenesis in the Early Zebrafish Embryo (2010)
  • Author(s): Okuda, Y., Kondoh, H., Kamachi, Y.
  • Organizer: 69th Society for Developmental Biology Annual Meeting
  • Place of Presentation: Albuquerque USA
  • Year and Date: 2010-08-07
• [Presentation] SOX‐PAX転写因子の協調相互作用が関与する発生的遺伝子調節 (2010)
  • Author(s): 三上智之, ANDRABI Munazah, 蒲池雄介, 内川昌則, 近藤寿人
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] Sox11 に依存した miRNA-17-92 クラスター発現の制御機構
  • Author(s): 川合 杏奈, 近藤 寿人, 蒲池 雄介
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県）
• [Book] 分子生物学第3版, 田沼靖一編, (発生と細胞分化) (2011)
  • Author(s): 蒲池雄介
  • Publisher: 丸善出版
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/06/