Optogenetic analysis of neural functions in zebrafish

• Project/Area Number: 22500299
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute of Genetics (2011-2012); 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター、DBCLS) (2010)
• Principal Investigator: MUTO Akira 国立遺伝学研究所, 個体遺伝研究系, 助教 (00525991)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ゼブラフィッシュ / カルシウムイメージング / GCaMP / 視覚 / 脳 / 神経回路 / 光遺伝学 / オプトジェネティクス / チャネルロドプシン / ハロロドプシン / 視覚系 / 視蓋 / 捕獲行動 / 行動学 / 画像解析 / 眼球運動 / カルシウム / 脊髄 / 運動神経 / イメージング / Ga14 / UAS

Research Abstract

To understand how the brain works, it is desirable to visualize neuronal activity in the neuronal network and to evaluate the effect of blockade or stimulation of neuronal activity. Electrical activity of the neurons can be indirectly monitored by measuring the calcium influx via voltage-dependent calcium channels. We used zebrafish larvae as a model system to visualize neuronal activity in a specific subset of neurons. Furthermore, we developed optogenetic tools that can be applied in manipulation of neuronal activity

Research Products

• [Journal Article] Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system. (2013)
  • Author(s): Umeda K, Shoji W Sakai S, Muto A, Kawakami K, Ishizuka T, and Yawo H
  • Journal Title: Neuroscience Research Volume: 75 Issue: 1 Pages: 69-75
  • DOI: 10.1016/j.neures.2012.08.010
  • Peer Reviewed
• [Journal Article] Connexin 39.9 protein is necessary for coordinated activation of slow-twitch muscle and normal behavior in zebrafish (2012)
  • Author(s): Hirata H, Wen H, Kawakami Y, Naganawa Y, Ogino K, Yamada K, Saint-Amant L, Low SE, Cui WW, Zhou W, Sprague SM, Asakawa K, Muto A, Kawakami K, Kuwada JY
  • Journal Title: J Biol Chem Volume: 287(2) Issue: 2 Pages: 1080-9
  • DOI: 10.1074/jbc.m111.308205
  • Peer Reviewed
• [Journal Article] Imaging functional neural circuits in zebrafish with a new GCaMP and the Gal4FF-UAS system (2011)
  • Author(s): Muto A, Kawakami K
  • Journal Title: Communicative & integrative biology Volume: 4 Pages: 566-568
  • Peer Reviewed
• [Journal Article] Higashijima S, Nakai J,and Kawakami K (2011)
  • Author(s): Muto A, Ohkura M, Kotani T
  • Journal Title: Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish
  • Peer Reviewed
• [Journal Article] Imaging functional neural circuits in zebrafish with a new GCaMP and the Gal4FF-UAS system (2011)
  • Author(s): Muto A, Kawakami K
  • Journal Title: Commun Integr Biol Volume: 4(5) Pages: 566-568
• [Journal Article] Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish (2011)
  • Author(s): Muto, A, et al.
  • Journal Title: Proceedings of the National Academy of Sciences, U.S.A Volume: 108(13) Pages: 5425-5430
  • Peer Reviewed
• [Journal Article] Imaging functional neural circuits in zebrafish with a new GCaMP and the Gal4FF-UAS system (2011)
  • Author(s): Muto, A, Kawakami, K.
  • Journal Title: Communicative & Integrative Biology Volume: 4(5)(in press)
• [Journal Article] zTrap: zebrafish gene trap and enhancer trap database (2010)
  • Author(s): Kawakami K, Abe G, Asada T, Asakawa K, Fukuda R, Ito A, Lal P, Mouri N, Muto A, Suster ML, Takakubo H, Urasaki A, Wada H, Yoshida M
  • Journal Title: BMC developmental biology 10 105 Oct 2010 Volume: 105 Issue: 1 Pages: 105-105
  • DOI: 10.1186/1471-213x-10-105
  • Peer Reviewed
• [Presentation] Visualization of functional neural circuits in zebrafish (2012)
  • Author(s): Muto A, and Kawakami K
  • Organizer: Tenth International Congress of Neuroethology
  • Place of Presentation: USA
• [Presentation] perception of a natural object (2012)
  • Author(s): Muto A, Ohkura M, Abe G, Nakai J, Kawakami K
  • Organizer: 10th International Zebrafish Genetics and Development
  • Place of Presentation: Madison, Wisconsin, USA.
• [Presentation] Visualization of functional neural circuits in zebrafish. Tenth International Congress of Neuroethology. (2012)
  • Author(s): Akira Muto
  • Organizer: Tenth International Congress of Neuroethology
  • Place of Presentation: College Park, MD USA
  • Invited
• [Presentation] Probing visual perception in zebrafish with the improved GCaMP (2011)
  • Author(s): Muto A
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama, Japan
• [Presentation] Functional brain imaging with improved GCaMPs in zebrafish (2011)
  • Author(s): Muto A, Nakai J, and Kawakami K
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington DC, USA
• [Presentation] Calcium imaging of the zebrafish visual system with the GCaMP (2011)
  • Author(s): Muto A, Nakai J, Kawakami K
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜パシフィコ
• [Presentation] Calcium imaging of the zebrafish brain with improved GCaMPs (2011)
  • Author(s): Muto A, and Kawakami K
  • Organizer: 7th European Zebraish Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Probing visual perception in zebrafish with the improved GCaMP (2011)
  • Author(s): Muto A.
  • Organizer: The 34^<th> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama, Japan(招待講演)
• [Presentation] Calcium imaging of the zebrafish visual system with the GCaMP (2011)
  • Author(s): Muto A, Nakai J, Kawakami K
  • Organizer: The 34th Annual meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama, Japan
• [Presentation] Brain imaging with improved GCaMPs in zebrafish (2010)
  • Author(s): Muto, A., et al.
  • Organizer: The 33rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Brain Imaging with Improved GCaMPs in Zebrafish (2010)
  • Author(s): Muto A, Nakai J, Kawakami K
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] Visualization of neuronal activity in zebrafish spinal neurons with an improved GCaMP (2010)
  • Author(s): Muto A, Ohkura M, Nakai J, and Kawakami K
  • Organizer: International Meeting on Zebrafish Genetics and Development. Madison, WI, USA. June 16-20,
  • Place of Presentation: Madison, WI, USA
• [Remarks]
  • URL: http://kawakami.lab.nig.ac.jp/