| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The specific aim of this study was to classify and characterize the excitatory pyramidal neurons that constitute the cortical circuit. I obtained the following two new findings. (1) By combining the retrograde tracers with the in situ hybridization method, I found that CCK and PCP4 genes are expressed in the cortical and thalamic projection neurons in layer 6. Furthermore, the area differences of their distribution were revealed. (2) I developed a novel lentiviral-based retrograde vector system that carries TET-Off components for high-level transgene expression. This vector was used to visualize the callossal neurons of the marmoset (New World monkey) cortex.
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