Study on cortical circuit using viral vectors:Species/area-comparison of layer 6 neurons

• Project/Area Number: 22500300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute for Basic Biology
• Principal Investigator: WATAKABE Akiya 基礎生物学研究所, 脳生物学研究部門, 准教授 (40290910)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 大脳皮質回路 / ウイルスベクター / 蛍光タンパク質 / レンチウイルス / 逆行性感染 / TET誘導 / 6層ニューロン / マーモセット

Research Abstract

The specific aim of this study was to classify and characterize the excitatory pyramidal neurons that constitute the cortical circuit. I obtained the following two new findings. (1) By combining the retrograde tracers with the in situ hybridization method, I found that CCK and PCP4 genes are expressed in the cortical and thalamic projection neurons in layer 6. Furthermore, the area differences of their distribution were revealed. (2) I developed a novel lentiviral-based retrograde vector system that carries TET-Off components for high-level transgene expression. This vector was used to visualize the callossal neurons of the marmoset (New World monkey) cortex.

Research Products

• [Journal Article] Genetic dissection of the circuit for hand dexterity in primates (2012)
  • Author(s): Kinoshita M, Matsui R, Kato S, Hasegawa T, Kasahara H, Isa, K, Watakabe A, Yamamori, T, Nishimura, Y, Alstermark, B, Watanabe, D, Kobayashi, K, Isa, T
  • Journal Title: Nature Volume: 487 Pages: 235-238
  • Peer Reviewed
• [Journal Article] Area-specific substratification of deep layer neurons in the rat cortex (2012)
  • Author(s): Watakabe A, Hirokawa J, Ichinohe N, Ohsawa S, Kaneko T , Rockland, KS, Yamamori, T
  • Journal Title: J Comp Neurol Volume: 520 Pages: 3553-3573
  • Peer Reviewed
• [Journal Article] Visualization of Cortical Projection Neurons with Retrograde TET-Off Lentiviral Vector (2012)
  • Author(s): Watakabe A, Kato S, Kobayashi K, Takaji M, Nakagami Y, Y, Sadakane, O, Ohtsuka, M, Hioki, H, Kaneko, T, Okuno, H, Kawashima, T, Bito, H, Kitamura, Y, Yamamori, T
  • Journal Title: PLoS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] Area-Specific Substratification of Deep Layer Neurons in the Rat Cortex (2012)
  • Author(s): Akiya Watakabe, Junya Hirokawa, Noritaka Ichinohe, Sonoko Ohsawa, Takeshi Kaneko, Kathleen S. Rockland, and Tetsuo Yamamori
  • Journal Title: The Journal of Comparative Neurology Volume: 520(16) Issue: 16 Pages: 3553-3573
  • DOI: 10.1002/cne.23160
  • Peer Reviewed
• [Journal Article] Visualization of the cortical projection neurons by retrograde TET-OFF lentiviral vector. (2012)
  • Author(s): 渡我部昭哉, 他
  • Journal Title: PLos One Volume: 7 Issue: 10 Pages: e46157-e46157
  • DOI: 10.1371/journal.pone.0046157
  • Peer Reviewed
• [Journal Article] Altered layer-specific gene expression in cortical samples from patients with temporal lobe epilepsy (2011)
  • Author(s): Rossini L, Moroni RF, Tassi L, Watakabe A, Yamamori T, Spreafico, R, Garbelli, R
  • Journal Title: Epilepsia Volume: 52 Pages: 1928-1937
  • Peer Reviewed
• [Journal Article] クローズアップ実験法 (2011)
  • Author(s): 渡我部 昭哉/小松勇介/山森哲雄
  • Journal Title: 脳の蛍光in situ hybridization法 Volume: 2月号
• [Journal Article] 脳の蛍光in situ hybridization法 (2011)
  • Author(s): 渡我部, 昭哉, 他2名
  • Journal Title: 実験医学 Volume: 29 Pages: 467-472
• [Journal Article] Fluorescent in situ hybridization technique for cell type identification and characterization in the central nervous system (2010)
  • Author(s): Watakabe A, Komatsu Y, Ohsawa S, Yamamori T
  • Journal Title: Methods Volume: 52 Pages: 367-374
  • Peer Reviewed
• [Journal Article] Fluorescent in situ hybridization technique for cell type identification and characterization in the central nervous system. (2010)
  • Author(s): Akiya Watakabe
  • Journal Title: Methods Volume: 52(invite) Pages: 367-374
  • Peer Reviewed
• [Presentation] Differential labeling of the cortical projection neuron subpopulations by TET-double infection method (TEDI) (2012)
  • Author(s): 渡我部 昭哉他6名
  • Organizer: 北米神経学会
  • Place of Presentation: 米国ニューオーリンス"にてポスター発表
• [Presentation] Differential labeling of the cortical projection neuron subpopulations by TET-double infection method (TEDI). (2012)
  • Author(s): Akiya Watakabe, Shigeki Kato, Kazuto Kobayashi, Hiroaki Mizukami, Keiya, Ozawa, Tetsuo Yamamori (Akiya Watakabe)
  • Organizer: 北米神経学会
  • Place of Presentation: 米国ニューオーリンズ
• [Presentation] TET2重感染法(TEDI)による皮質視床投射ニューロン形態の可視化 (2011)
  • Author(s): 渡我部 昭哉他5名
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜(神奈川県)にてポスター発表。
  • Year and Date: 2011-09-16
• [Presentation] TET2重感染法(TEDI)による皮質視床投射ニューロン形態の可視化 (2011)
  • Author(s): 渡我部昭哉, 他5名
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] "TET-OFF" LENTIVIRAL VECTORS DRIVE HIGH-LEVEL TRANSGENE EXPRESSION IN MARMOSET BRAINS (2010)
  • Author(s): 渡我部 昭哉他11名
  • Organizer: 北米神経学会(Neuroscience 2010)
  • Place of Presentation: San Diego(米国)にてポスター発表
  • Year and Date: 2010-11-16
• [Presentation] "TET-OFF" LENTIVIRAL VECTORS DRIVE HIGH-LEVEL TRANSGENE EXPRESSION IN MARMOSET BRAINS (2010)
  • Author(s): 渡我部, 昭哉, 他11名
  • Organizer: 北米神経学会(Neuroscience 2010)
  • Place of Presentation: San Diego(米国)
  • Year and Date: 2010-11-16
• [Presentation] TET-OFFレンチウイルスベクターによるマーモセット脳への遺伝子導入 (2010)
  • Author(s): 渡我部 昭哉他11名
  • Organizer: 第33回日本神経科学学会(Neuro2010)
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)にてポスター発表
  • Year and Date: 2010-09-02
• [Presentation] TET-OFFレンチウイルスベクターによるマーモセット脳への遺伝子導入 (2010)
  • Author(s): 渡我部, 昭哉, 他11名
  • Organizer: 第33回日本神経科学学会(Neuro2010)
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-02
• [Remarks]
  • URL: http://www.nibb.ac.jp/brish
• [Remarks] 本研究に用いているin situ hybridization法の普及を目的としたホームページの更新。
• [Remarks] 霊長類の神経回路を可視化する新しいツールを開発
  • URL: http://www.nibb.ac.jp/pressroom/news/2012/10/121006.html
• [Remarks]
  • URL: http://www.nibb.ac.jp/%7Edivspe1/
• [Remarks]
  • URL: http://www.nibb.ac.jp/%7Edivspe1/