The BDNF pro-peptide is a novel facilitator of hippocampal LTD and the biological action is altered by a common BDNF polymorphism Val66Met

• Project/Area Number: 22500305
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KOJIMA Masami 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (40344171)
• Co-Investigator(Kenkyū-buntansha): UEGAKI Kouichi 独立行政法人産業技術総合研究所, 生体分子創製研究グループ, 研究グループ長 (00356544) ; ISHIKAWA Yasuyuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教授 (90346320)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経栄養因子 / 神経細胞 / 神経伝達 / プロセッシング / 分泌 / シナプス / 成長因子 / 一塩基多型 / 海馬 / LTD / endocytosis / 細胞イメージング

Research Abstract

Most growth factors are initially synthesized as precursors and subsequently processed into their mature form and its pro-peptide by proteolytic cleavage in the secretory pathway and extracellular spaces. Compared to that of the mature form, the biologica

Research Products

• [Journal Article] BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons (2013)
  • Author(s): S. Dieni
  • Journal Title: Journal of cell Biology Volume: 16 Issue: 6 Pages: 339-350
  • DOI: 10.1083/jcb.201201038
  • Peer Reviewed
• [Journal Article] BDNF and Synaptic Plasticity: The Recent Cell Biology for Understanding of Brain Disorders (2013)
  • Author(s): T. Mizui
  • Journal Title: Clinical Pharmacology & Biopharmaceutics Volume: 5 Pages: 50-52
  • Peer Reviewed
• [Journal Article] BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons (2012)
  • Author(s): S. Dieni, T. Matsumoto, M. Dekkers, S. Rauskolb, M. Ionescu, R. Deogracias, E. D.Gundelfinger, M. Kojima, S. Nestel, M. Frotscher, Y.-A. Barde
  • Journal Title: Journal of cell Biology Volume: vol. 196 no. 6 Pages: 775-788
• [Journal Article] Generation of TrkA/TrkB Chimeric Receptor Constructs Reveals Molecular Mechanisms underlying BDNF-Induced Dendritic Outgrowth in Hippocampal Neurons (2011)
  • Author(s): Yosuke Sato, Shingo Suzuki, Mako Kitabatake,,Tomoko Hara, and Masami Kojima
  • Journal Title: CELL.MOL.NEUROBIOL Volume: vol.31,no.605-614
• [Journal Article] Generation of TrkA/TrkB Chimeric Receptor Constructs Reveals Molecular Mechanisms underlying BDNF-Induced Dendritic Outgrowth in Hippocampal Neurons (2011)
  • Author(s): Y.Sato, et al
  • Journal Title: Cell.Mol.Neurobiol. Volume: 31 Issue: 4 Pages: 605-614
  • DOI: 10.1007/s10571-011-9655-8
  • Peer Reviewed
• [Journal Article] Generation of TrkA/TrkB Chimeric Receptor Constructs Reveals Molecular Mechanisms underlying BDNF-Induced Dendritic Outgrowth in Hippocampal Neurons. (2011)
  • Author(s): Yosuke Sato, Shingo Suzuki, Mako Kitabatake, Tomoko Hara, Masami Kojima
  • Journal Title: CELL.MOL.NEUROBIOL. Volume: 31 Pages: 605-614
  • Peer Reviewed
• [Journal Article] Distinct signaling pathways of precursor BDNF and mature BDNF in cultured cerebellar granule neurons (2010)
  • Author(s): Koshimizu, H., Hazama, S., Hara, T., Ogura, A., and Kojima, M
  • Journal Title: Neurosci Lett Volume: vol.473 no.229-232
• [Journal Article] うつ病に関するBDNF研究:現状と課題 (2010)
  • Author(s): 熊ノ郷晴子、水井利幸、高橋正身、小島正己
  • Journal Title: 実験医学 Volume: 28巻 Pages: 89-95
• [Journal Article] Distinct signaling pathways of precursor BDNF and mature BDNF in cultured cerebellar granule neurons (2010)
  • Author(s): Koshimizu, H., Hazama, S., Hara, T., Ogura, A., Kojima, M.
  • Journal Title: Neurosci Lett. Volume: 473 Pages: 229-232
  • Peer Reviewed
• [Journal Article] ultiple functions of precursor BDNF to CNS neurons: negative regulation of neurite growth, spine formation and cell survival (2009)
  • Author(s): Koshimizu, H., Kiyosue, K., Hara, T.,Hazama, S., Suzuki, S., Uegaki, K., Nagappan, G., Zaitsev, E., Hirokawa, T., Tatsu, Y., Ogura, A., Lu, B. & Kojima, M
  • Journal Title: Mol. Brain Volume: vol.2,no.1-19
• [Journal Article] The BDNF pro-peptide is a novelfacilitator of hippocampal LTD and the biological action is modulated by a common BDNF polymorphism Val66Met
  • Author(s): Uegaki K., Ishikawa Y., Mizui T., Hirokawa T., Hara T., Suzuki S., Takahashi M., Shiosaka S., Itami C., Kumanogoh H., Kojima M
  • Volume: (in press)
• [Presentation] The BDNF pro-peptide is a novel facilitator of hippocampal LTD and the biological action is modulated by a common BDNF polymorphism Val66Met (2013)
  • Author(s): Uegaki K., Ishikawa Y., Mizui T., Kumanogoh H., Kojima M
  • Organizer: GRC
  • Place of Presentation: New Port
• [Presentation] The BDNF pro-peptide is a novel facilitator of hippocampal LTD and the biological action is modulated by a common BDNF polymorphism Val66Me (2012)
  • Author(s): M. Kojima
  • Organizer: NGF2012
  • Place of Presentation: ドイツ ヴュルツブルク
• [Presentation] A novel role of the BDNF pro-peptide in the development of neuron : a new insight into neurotrophic hypothesis (2012)
  • Author(s): T. Kozuka
  • Organizer: The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry
  • Place of Presentation: 兵庫県 神戸市
• [Presentation] BDNF and mood disorders (2011)
  • Author(s): Masami Kojima
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-17
• [Presentation] BDNF and mood disorders (2011)
  • Author(s): Masami Kojima
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
  • Year and Date: 2011-09-17
• [Presentation] The BDNF pro-peptide is a new modulator of synaptic plasticity in the nervous system (2010)
  • Author(s): Masami Kojima
  • Organizer: NGF 2010
  • Place of Presentation: Helsinki, Finland
  • Year and Date: 2010-06-10
• [Presentation] 神経栄養因子 BDNF の機能未知ドメインが行う脳機能調節 (2009)
  • Author(s): 小島正己
  • Organizer: 生理研研究会
  • Place of Presentation: 生理学研究所(岡崎市)
  • Year and Date: 2009-06-19
• [Book] うつ病に関する BDNF 研究:現状と課題 (2010)
  • Author(s): 熊ノ郷晴子、水井利幸、高橋正身、小島正己
  • Total Pages: 7
  • Publisher: 実験医学
• [Book] The role of brain-derived neurotrophic factor as a modulator of synaptic plasticity, Brain Aging
  • Author(s): Y. Ishikawa, M. Kojima
  • Publisher: Therapeutic Interventions
• [Remarks]
  • URL: http://www.aist.go.jp/
• [Remarks] バイオインターフェース研究グループ
  • URL: http://unit.aist.go.jp/hri/
• [Remarks]
  • URL: http://unit.aist.go.jp/hri/
• [Remarks]
  • URL: http://unit.aist.go.jp/hri/group/birg/
• [Patent(Industrial Property Rights)] 成長因子ペプチド (2010)
  • Inventor(s): 上垣浩一、水井利幸、熊ノ郷 晴子、原とも子、石川保幸、小島正己
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Filing Date: 2010-05-31
• [Patent(Industrial Property Rights)] 神経細胞死促進因子及び該因子を高感度に検出する方法 (2010)
  • Inventor(s): 小島正己、原とも子、小清水久嗣、上垣浩一、柏原めぐみ
  • Industrial Property Rights Holder: (独)産業技術総合研究所
  • Acquisition Date: 2010-02-19
• [Patent(Industrial Property Rights)] 成長因子のプロペプチド (2010)
  • Inventor(s): 小島正己、水井利幸、熊ノ郷晴子、北畠真子、上垣浩一、原とも子
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Industrial Property Number: 2010-123957
  • Filing Date: 2010-05-31