Analysesonthe projection pattern of theseptohippocampal cholinergic pathwayand its degeneratingprocess.

• Project/Area Number: 22500308
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: YASUHARA Osamu 滋賀県立大学, 人間看護学部, 教授 (80239772)
• Co-Investigator(Kenkyū-buntansha): MATSUO Akinori 滋賀医科大学, 分子神経科学研究センター, 助教 (20324585) ; MATSUDA Wakoto 滋賀医科大学, 医学部, 助教 (80444446)
• Co-Investigator(Renkei-kenkyūsha): FRUTA Takahiro 京都大学, 医学研究科, 助教 (60314184)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 神経回路網 / コリン作動性神経 / アセチルコリン / 内側中隔 / 海馬 / カベオリン / 神経トレース

Research Abstract

Dysfunction of the basal forebrain cholinergic system has drawn attention as a cause of cognitive deficits in human neurodegenerative diseases. In the present study, we tried to analyze functions, modes of projection, and degenerative processes of this cholinergic system.
1) To elucidate functions of nuclear choline acetyltransferase (ChAT; a synthesizing enzyme of acetylcholine), we investigated the alteration of candidate gene transcription by ChAT, and found that nuclear ChAT enhanced transcription of high affinity choline transporter gene.
2) The projection mode of septal cholinergic neurons was analyzed using sindvis virus vector.
3) Because caveolin 1 has been implicated in the central cholinergic transmission, its expression change was examined in kainic acid-induced hippocampal damage in rats. Expression of caveolin 1 was induced in activated microglia by kainic acid, while no evidence was found for any role of caveolin 1 incentral cholinergicfunctions.

Research Products

• [Journal Article] Kainic acid induces expressionofcaveolin-1in activated microglia in rat brain (2013)
  • Author(s): Takeuchi, S., Matsuda, W., Tooyama, I., Yasuhara, O.
  • Journal Title: Folia Histochem. Cytobiol. Volume: 51 Issue: 1 Pages: 25-30
  • DOI: 10.5603/fhc.2013.004
  • Peer Reviewed
• [Journal Article] Kainic acid induces expression of caveolin-1 in activated microglia in rat brain. (2013)
  • Author(s): Takeuchi, S., Matsuda, W., Tooyama, I., Yasuhara. O.
  • Journal Title: Folia Histochemica et Cytobiologica Volume: 51
  • Peer Reviewed
• [Journal Article] Human neural stem cells over-expressing choline acetyltransferase restore cognition in rat model of cognitive dysfunction. (2012)
  • Author(s): Park, D., Lee, H.J., Joo,S.S.,Bae, D-K., Yang. G., Yang, Y-H., Lim, I., Matsuo, A., Tooyama, I., Kim, Y-B., Kim, S.U.
  • Journal Title: Exp. Neurol. Volume: 234 Pages: 521-526
  • Peer Reviewed
• [Journal Article] Human neural stem cells overexpressing choline acetyltransferase restore cognitive function of kainic acid-induced learning and momorydeficit animals (2012)
  • Author(s): Park, D., Joo, S.S., Kim,T.K.,Lee, S.H., Kang, H., Lee, H.J., Lim,I., Matsuo, A., Tooyama, I., Kim, Y-B., Kim, S.U.
  • Journal Title: Cell Transplantation 21 Pages: 365-371
  • URL: http://dx.doi.org/10.3272/096368911X586765
  • Peer Reviewed
• [Journal Article] Human neural stem cells overexpressing choline acetyltransferaserestore cognitive function of kainic-acid-induced learning and memory deficit animals (2012)
  • Author(s): Park D, Matuso A, et al
  • Journal Title: Cell Transplantation Volume: 21 Pages: 365-371
  • Peer Reviewed
• [Journal Article] Human neural stem cells overexpressing choline acetyltransferaserestore cognition in rat model of cognitive dysfunction (2012)
  • Author(s): Park D, Matuso A, et al
  • Journal Title: Exp.Neurol. Volume: 234 Pages: 521-526
  • Peer Reviewed
• [Journal Article] Nuclear choline acetyltransferase activates transcription of a high-affinity choline transporter (2011)
  • Author(s): Matsuo, A., Bellier, J-P.,Nishimura, M., Yasuhara, O., Saito, N., Kimura, H.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 5836-5845
  • Peer Reviewed
• [Journal Article] Nuclear choline acetyltransferase activiates transcription of a high-affinity choline transporter (2011)
  • Author(s): Matsuo A, Bellier JP, Yasuhara O, et al.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 5836-5845
  • Peer Reviewed
• [Presentation] uclearcholine acetyltransferase activates transcription of a high-affinity choline transporter (2011)
  • Author(s): Matsuo, A., Bellier, J-P.,Nishimura, M., Saito, N., Yasuhara, O., Kimura, H.
  • Organizer: 8th IBROWorldCongressof Neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-14