| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
To investigate molecular mechanisms of formation of complex and organized dendrites by cerebellar Purkinje cells, we introduced siRNAs of various genes into cultured Purkinje cells using single-cell electroporation. We found that ryanodine receptor, CaM kinases and myosin Va are involved in dendrite formation of Purkinje cells in their own manners. In addition, we established the forced gene expression system using single-cell electroporation and the coculture system of a cerebellar slice and dissociated Purkinje cells.
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