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Molecular mechanisms underlying axonal local protein synthesis in neurons

Research Project

Project/Area Number 22500329
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNiigata University

Principal Investigator

TAKEI Nobuyuki  新潟大学, 脳研究所, 准教授 (70221372)

Co-Investigator(Renkei-kenkyūsha) NAMBA Hisaaki  新潟大学, 脳研究所, 助教 (90332650)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞内情報伝達 / 局所翻訳 / 蛋白合成 / 成長円錐 / 翻訳調節 / eIF2a / eIF2Be / 局所的翻訳 / BDNF / Wnt3a / eEF2 / GSK3b / eIF2Bε
Research Abstract

Translation machinery in growth cone. Existence of ribosomes in growth cone was investigated by immunocytochemistry with anti-ribosomalprotein antibodies. Although it is widely believed that there is no ribosomes in axon, immunoreactivityagainst ribosomal proteins was recognized in growth cone of cultured dorsal root ganglia neurons and ofhippocampal neurons. In addition, several translation factors were found in growth cone of theseneurons. Precise morphological analysis of growth cone was performed by using atomic forcemicroscopy.
Triggers of local translation in growth cone.Brain-derived neurotrophic factor (BDNF) and Wnt3a were identified as a trigger of local translation ingrowth cone. In addition to the [35S]methionin incorporation, puromycin-anti-puromycin immunocytochemistry method revealed that these factors activate novel protein synthesis in growth cones.
These results indicate the existence of translational machinery in growth cone and strongly suggest thatlocal protein synthesis occurs in growth cones in response to certain growth factors.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (25 results)

All 2013 2012 2011 2010 Other

All Journal Article (16 results) (of which Peer Reviewed: 7 results) Presentation (7 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Interactions between subcorticalheterotopia and overlying hippocampus mediated by NMDA receptors in humanbrain slices2012

    • Author(s)
      Kitaura H, Oishi M, Takei N, Fu YJ, Hiraishi T, Fukuda M, Takahashi H,Shibuki K, Fujii Y, and Kakita A
    • Journal Title

      Epilepsia Jul

      Volume: 53(7)

    • Related Report
      2012 Final Research Report
  • [Journal Article] Cell-surface expression of themajor Aβdegrading enzyme, neprilysin,depends on phosphorylation by MEK anddephosphorylation by protein phosphatase1a2012

    • Author(s)
      Kakiya N, Saito T, Nilsson P, Matsuba Y,Tsubuki S, Takei N, Nawa H, Saido TC
    • Journal Title

      J Biol Chem

      Volume: 287(35) Pages: 29362-72

    • Related Report
      2012 Final Research Report
  • [Journal Article] 神経細胞特異的翻訳調節機構「基礎の基礎」2012

    • Author(s)
      武井延之
    • Journal Title

      細胞工学

      Volume: 31 Pages: 642-645

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 神経細胞特異的翻訳調節機構「翻訳調節と脳高次機能」2012

    • Author(s)
      武井延之
    • Journal Title

      細胞工学

      Volume: 31 Pages: 687-691

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Periventricular nodular heterotopia functionally couples with the overlying hippocampus.2012

    • Author(s)
      Kitaura H
    • Journal Title

      Epilepsia

      Volume: 53 Issue: 7 Pages: 127-131

    • DOI

      10.1111/j.1528-1167.2012.03509.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell surface expression of the major amyloid-β peptide (Aβ)-degrading enzyme, neprilysin, depends on phosphorylation by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) and dephosphorylation by protein phosphatase 1a.2012

    • Author(s)
      Kakiya, Naomasa
    • Journal Title

      J.Biol.Chem.

      Volume: 287 Issue: 35 Pages: 29362-29572

    • DOI

      10.1074/jbc.m112.340372

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dopamine D1-like Receptor-dependent Ectodomain Shedding and Release of Epidermal Growth Factor in Developing Striatal Neurons: (Part2) Target-Derived Neurotrophic Signaling2011

    • Author(s)
      Iwakura Y., Wang, R., Abe, Y., Piao, Y., Shishido,Y., Higashiyama, S., Takei, N.and Nawa, H
    • Journal Title

      J Neurochem

      Volume: 118 Pages: 57-68

    • Related Report
      2012 Final Research Report
  • [Journal Article] Qualitative and Quantitative Re-evaluation of Epidermal Growth Factor-ErbB1 Action on Developing Midbrain Dopamine Neurons in vivo nd in vitro; (Part 1) Target-Derived Neurotrophic Signaling2011

    • Author(s)
      wakura, Y., Zheng, Y., Abe, Y., Piao, Y., Yokomaku, D., Wang, R., Takei, N. and Nawa, H
    • Journal Title

      J Neurochem

      Volume: 118 Pages: 45-46

    • Related Report
      2012 Final Research Report
  • [Journal Article] Protein Production of an Active Neurotrophic Factor, Neuregulin-1; Qualitative Comparison of In Vitro Translation Systems2011

    • Author(s)
      Wang R, Iwakura Y, Araki K, Sotoyama H, Takei N and Nawa H
    • Journal Title

      Neurosci Lett

      Volume: 497 Pages: 90-93

    • Related Report
      2012 Final Research Report
  • [Journal Article] Qualitative and quantitative re-evaluation of epidermal growth factor-ErbB1 action on developing midbrain dopaminergic neurons in vivo and in vitro : target-derived neurotrophic signaling (Part 1)2011

    • Author(s)
      Iwakura, et al
    • Journal Title

      Journal of Neurochemistry

      Volume: 118 Issue: 1 Pages: 45-56

    • DOI

      10.1111/j.1471-4159.2011.07287.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dopamine-dependent ectodomain shedding and release of epidermal growth factor in developing striatum : target-derived neurotrophic signaling (Part 2)2011

    • Author(s)
      Iwakura, et al.
    • Journal Title

      Journal of Neurochemistry

      Volume: 118 Issue: 1 Pages: 57-68

    • DOI

      10.1111/j.1471-4159.2011.07295.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro production of an active neurotrophic factor, neuregulin-1 : qualitative comparison of different cell-free translation systems2011

    • Author(s)
      Wang, et al
    • Journal Title

      Neuroscience Letters

      Volume: 497 Pages: 90-93

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ctivation of mammalian target of rapamycin signaling in spatial learning2010

    • Author(s)
      Qi, S., Mizuno, M., Yonezawa, K., Nawa, H. and Takei, N
    • Journal Title

      Neurosci. Res

      Volume: 68 Pages: 88-93

    • Related Report
      2012 Final Research Report
  • [Journal Article] Downregulation of NF-kB signaling by N-terminal mutant huntingtin proteins induces oxdative stress and cell death2010

    • Author(s)
      Reijonen, S., Kukkonen, J.P., Kairisalo, M., Takei, N., Lindholm, D. and Korhonen, L
    • Journal Title

      Cell Mol. Life Sci

      Volume: 67 Pages: 1929-194

    • Related Report
      2012 Final Research Report
  • [Journal Article] Activation of mammalian target of rapamycin signaling in spatial learning2010

    • Author(s)
      Qi, S, et al.
    • Journal Title

      Neurosci.Res.

      Volume: 68 Pages: 88-93

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of NF-kB signaling by N-terminal mutant of huntingtin proteins induces oxdative stress and cell death.2010

    • Author(s)
      Reijonen, S, et al.
    • Journal Title

      Cell.Mol.Life Sci.

      Volume: 67 Pages: 1929-19416

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ラット脊髄後根神経節細胞の成長円錐の神経栄養因子による構造変化2013

    • Author(s)
      星治
    • Organizer
      第118回日本解剖学会
    • Place of Presentation
      高松
    • Related Report
      2012 Annual Research Report
  • [Presentation] 神経細胞における翻訳調節2012

    • Author(s)
      武井延之
    • Organizer
      シンポジウム「シナプス伝達機構の理解」
    • Place of Presentation
      上智大学(招待講演)
    • Year and Date
      2012-03-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] PallidalHyperdopaminergic Innervation Induced by Neonatal Exposure To Epidermal Growth Factor; Implication in Schizophrenia2012

    • Author(s)
      Nawa, Hiroyuki
    • Organizer
      11th meeting of APSN(asia pacific society for neurochemistry)
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] AMP-activated protein kinase counteracts BDNF-induced mammalian target of rapamycin complex1 signaling and protein synthesis in neurons2012

    • Author(s)
      Ishizuka, Yuta
    • Organizer
      11th meeting of APSN(asia pacific society for neurochemistry)
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] 神経細胞ではAMPKが成長因子によるmTORC1シグナル活性化と蛋白合成促進をドミナントに制御する。2011

    • Author(s)
      武井延之
    • Organizer
      BMB2010(生化学会/分子生物学会合同年会)
    • Place of Presentation
      神戸
    • Year and Date
      2011-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 神経細胞における翻訳調節:活動依存性と空間的特性2011

    • Author(s)
      武井延之、那波宏之
    • Organizer
      84回生化学会シンポジウム「翻訳制御の多様性」
    • Place of Presentation
      京都(オーガナイザー)
    • Year and Date
      2011-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 神経細胞における翻訳調節:活動依存性と空間的特性2011

    • Author(s)
      武井延之、那波宏之
    • Organizer
      第84回日本生化学会シンポジウム2S6a「翻訳制御の多様性」
    • Place of Presentation
      京都国際会館(招待講演)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Book] 神経細胞特異的翻訳調節機構2012

    • Author(s)
      武井延之監修
    • Total Pages
      31
    • Related Report
      2012 Final Research Report
  • [Remarks] 新潟大学脳研究所

    • URL

      http://www.bri.niigata-u.ac.jp/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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