Regulatory mechanisms of central nervous system by Runx2
| Project/Area Number |
22500330
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HINOI Eiichi 金沢大学, 薬学系, 准教授 (70360865)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 神経細胞 / アストロサイト / Runx2 / コンディショナル欠損マウス / runx2 / グルタミン酸 / 小胞体ストレス / グルタミン酸トランスポーター |
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| Research Abstract |
We have previously shown the functional expression of glutamatergic and GABAergic signaling machineries in different osseous cells including osteoblasts. Runt-related factor 2 (Runx2) is the master regulator of osteoblastic differentiation with ability to accelerate differentiation of mesenchymal stem cells into osteoblasts, while we have also demonstrated the expression of mRNA and corresponding protein for Runx2. In this study, we for the first time generated mice carrying a conditional Runx2 allele with exon 4, which encodes the Runt domain, flanked by loxP sites. These mice were crossed with α1(I)-collagen-Cre or α1(II)-collagen-Cre transgenic mice to obtain osteoblast- or chondrocyte-specific Runx2 deficient mice, respectively. In newborn α1(II)-Cre;Runx2^flox/flox mice, mineralization impairment was restricted to skeletal areas undergoing endochondral ossification including long bones and vertebrae. In contrast, no apparent skeletal abnormalities were seen in mutant embryo, newborn, and 3- to 6-week old-mice in which Runx2 had been deleted with the α1(I)-collagen-Cre driver. The Runx2 floxed allele established here is undoubtedly useful for investigating the role of Runx2 in particular cells.
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Report
(4 results)
Research Products
(59 results)
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[Journal Article] An analysis of skeletal development in osteoblast- and chondrocyte-specific Runx2 knockout mice2013
Author(s)
Takeshi Takarada, Eiichi Hinoi, Ryota Nakazato, Hiroki Ochi, Cheng Xu, Azusa Tsuchikane, Shu Takeda, Gerard Karsenty, Takaya Abe, Hiroshi Kiyonari, and Yukio Yoneda
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Journal Title
J. Bone Miner. Res
Volume: 未定
Issue: 10
Pages: 2064-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Myosin VI reduces proliferation, but not differentiation, in pluripotent P19 cells2013
Author(s)
Takeshi Takarada, Miki Kou, Noritaka Nakamichi, Masato Ogura, Yuma Ito, Ryo Fukumori, Hiroshi Kokubo, Gabriela B. Acosta, Eiichi Hinoi and Yukio Yoneda
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Journal Title
PLoS ONE
Volume: 8
Issue: 5
Pages: e63947-e63947
DOI
Related Report
Peer Reviewed
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[Presentation] 時計遺伝子と骨組織2010
Author(s)
宝田剛志
Organizer
金沢大学薬学シンポジウム2010
Place of Presentation
金沢大学(石川県)(招待講演)
Year and Date
2010-12-02
Related Report
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