Elucidation of a novel process in neurotransmitter release

• Project/Area Number: 22500334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kobe University
• Principal Investigator: SAKISAKA Toshiaki 神戸大学, 大学院・医学研究科, 教授 (80359843)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経伝達物質の放出機構 / SNARE複合体 / トモシン / 小胞融合 / SNARE

Research Abstract

The main process in synaptic transmission is neurotransmitter release. It is important to elucidate the processes of neurotransmitter release for understanding synaptic plasticity and memory formation. So far, we have revealed that a SNARE regulator protein named tomosyn negatively regulates neurotransmitter release. In this study, we showed that tomosyn directly binds to Ca^<2+> sensor protein named synaptotagmin-1 in a Ca^<2+>-dependent manner. Tomosyn negatively regulates synaptotagmin-1-mediated step of Ca^<2+>-dependent neurotransmitter release. Tomosyn-synaptotagmin1 complex forms a novel process named closing and regulates continuous neurotransmitter release.

Research Products

• [Journal Article] Dual regulation of RA-RhoGAP activity by phosphatidic acid and Rap1 during neurite outgrowth (2011)
  • Author(s): Kurooka, T., Yamamoto, Y., Takai, Y., and Sakisaka, T.
  • Journal Title: J. Biol. Chem Volume: 286巻 Pages: 6832-6848
  • Peer Reviewed
• [Journal Article] Dual regulation of RA-RhoGAP activity by phosphatidic acid and Rapl during neurite outgrowth (2011)
  • Author(s): Takao Kurooka
  • Journal Title: The Journal of Biological Chemistry Volume: 286巻 Pages: 6832-6843
  • Peer Reviewed
• [Journal Article] Tomosyn inhibits synaptotagmin-1-mediated step of Ca^<2+>-dependent neurotransmitter release through its N-terminal WD40 repeats (2010)
  • Author(s): Yamamoto, Y., Mochida, S., Miyazaki, N., Kawai, K., Fujikura, K., Kurooka, T., Iwasaki, K., and Sakisaka, T.
  • Journal Title: J. Biol. Chem Volume: 285巻 Pages: 40943-40955
  • Peer Reviewed
• [Journal Article] The tail domain of tomosyn controls membrane fusion through tomosyn displacement by VAMP2 (2010)
  • Author(s): Yamamoto, Y., Fujikura, K., Sakaue, M., Okimura, K., Kobayashi, Y., Nakamura, T., and Sakisaka, T.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 399巻 Pages: 24-30
  • Peer Reviewed
• [Journal Article] Tomosyn inhibits synaptotagmin-1-mediated step of Ca^<2+>-dependent neurotransmitter release through its N-terminal WD40 repeats (2010)
  • Author(s): Yasunori Yamamoto
  • Journal Title: The Journal of Biological Chemistry Volume: 285巻 Pages: 40943-40955
  • Peer Reviewed
• [Journal Article] The tail domain of tomosyn controls membrane fusion through tomosyn displacement by VAMP2 (2010)
  • Author(s): Yasunori Yamamoto
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 339巻 Pages: 24-30
  • Peer Reviewed
• [Presentation] 小胞体局在タンパク質p38による尾部アンカー型膜タンパク質の生合成調節機構 (2011)
  • Author(s): 山本泰憲、匂坂敏朗
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-24
• [Presentation] 小胞体局在タンパク質p38による尾部アンカー型膜タンパク質の生合成調節機構 (2011)
  • Author(s): 山木泰憲
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-24
• [Presentation] シナプス小胞エキソサイトーシスにおけるSNARE制御分子トモシンの役割 (2010)
  • Author(s): 山本泰憲、藤倉航平、阪上海央、置村健二郎、小林雄太、中村俊宏、匂坂敏朗
  • Organizer: BMB2010第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-12-07
• [Presentation] シナプス小胞エキソサイトーシスにおけるSNARE制御分子トモシンの役割 (2010)
  • Author(s): 山本泰憲
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-07
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/membrd/
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/membrd/
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/membrd/