Regulation of nitric oxide / reactive oxygen species signal by neuronal nitric oxide synthase
| Project/Area Number |
22500337
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
IHARA Hideshi 大阪府立大学, 大学院・理学系研究科, 准教授 (60254447)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細胞内情報伝達 / 一酸化窒素-活性酸素 / 神経型一酸化窒素合成酵素 / 活性酸素種 / 一酸化窒素 / 8-ニトロ-cGMP / MPP+神経毒性 / H-Ras / 硫化水素 / NO-ROSシグナル / 活性酸素 / 一酸化窒素合成酵素 / 8-ニトロcGMP / MPP^+神経毒性 / MAPキナーゼ / 8ニトロcGMP / スプライシング変異体 / 質量分析法 / 蛍光免疫染色法 |
|---|
| Research Abstract |
nNOS producs not only NO, but also ROS through uncoupling reaction. In this study, we investigated NO/ROS signaling, a novel concept for cellular signal transduction. In PC12 cells expressing nNOS splice variants, treatment with MPP+activated the NO/ROS signaling to form a second messenger, 8-nitro-cGMP. We found a novel signal pathway leading to cell death through 8-nitro-cGMP formation.
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Report
(4 results)
Research Products
(85 results)
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[Journal Article] The critical role of nitric oxide signaling, via protein S-guanylation and nitrated cyclic GMP, in the antioxidant adaptive response2010
Author(s)
Fujii S, Sawa T, Ihara H, Tong KI, Ida T, Okamoto T, Ahtesham AK, Ishima Y, Motohashi H, Yamamoto M, Akaike T
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Journal Title
J.Biol.Chem.
Volume: 285
Pages: 23970-84
Related Report
Peer Reviewed
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[Book] 実験医学別冊2013
Author(s)
(澤智裕), 居原秀, 赤池孝章
Total Pages
10
Publisher
細胞の酸化ストレスを量る
Related Report
