Regulation of neurotrophin secretion by exocytosis

• Project/Area Number: 22500344
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: MATSUDA Naoto 東京大学, 医科学研究所, 特任准教授 (40313100)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 神経科学 / 神経栄養因子 / 開口放出

Research Abstract

We aimed to establish experimental systems to observe BDNF secretion in living animals to elucidate the changes in brain functions associated with BDNF secretion. (1) We tried to express BDNF-pHluorin based on the zebrafish homolog in the visual system of living zebrafish larvae. However, we failed to observe significant fluorescence of BDNF-pHluorin. (2) We successfully expressed BDNF-pHluorin in the mouse hippocampus by lentivirus or electroporation, which enables us to investigate BDNF secretion in relation to memory formation.