| Project/Area Number |
22500357
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 嗅覚マスキング / LogD / 匂い分子 / CNGチャネル / 光活性化物質 / 神経生理学 / 電気生理学 / パッチクランプ法 / 嗅細胞 / 脂溶性 / 細胞培養 / UVレーザー |
|---|
| Research Abstract |
Molecular diffusion in solutions is known to occur in the general neurons and cells. However, olfactory receptor cells have cilia, which having a nano-scale structure. In fact, olfactory transduction cascade exists within the cilia locally, it is important to know the spatial distribution and temporal kinetics of the transduction elements (cAMP and Ca2+) in the transduction cascade within the cilia in real-time. However, there were technical difficulties using nano-scale cilia. In the previous report, we overcame such technical limitation using patch clamp, nano-scale ROI, and fluorescent imaging (Fluo3 or 4) simultaneously. These results indicated that olfactory response was linear-summation at local in the cilia, which was caused by the existence of the diffusion limitation in the cilia. The olfactory masking showed presumably representing the integration of the substance into the hydrophobic site of the membrane.
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