| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
We previously showed in in vitro slice co-cultures of mice cerebral cortex and spinal cord that corticospinal (CS) synapses, once formed throughout the spinal cord, were eliminated from the ventral side during development in an NMDAR-dependent manner. This synapse elimination was regulated selectively by GluN2B (2B) and had critical period. In this study we first investigated whether this differential effect of 2B and 2A is due to difference in the amount of Ca2+ entry between these two types of subunits or to signaling molecules downstream to Ca2+ entry thereafter showed the involvement of CaMKII on the CS synapse elimination by using genetically modified mice. We further tried to identify the mechanism that closes the critical period and found that 2B decline is essential for closing the period.
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