The elucidation of the relationship between vascular smooth muscle contraction and cytoskeletal rearrangement by proteomics and functional analysis
| Project/Area Number |
22500364
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KISHI Hiroko 山口大学, 大学院・医学系研究科, 准教授 (40359899)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Sei 山口大学, 大学院・医学系研究科, 教授 (80225515)
KAJIYA Katsuko 山口大学, 大学院・医学系研究科, 講師 (00379942)
KAWAMICHI Hozumi 山口大学, 大学院・医学系研究科, 助教 (80363042)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 平滑筋生理 / 血管平滑筋 / 平滑筋収縮 / プロテオミクス / 細胞骨格 |
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| Research Abstract |
Abnormal vascular smooth muscle (VSM) contraction induces acute and fatal diseases such as angina pectoris, myocardial infarction and cerebral infarction. Using focused proteomics, we previously identified several cytoskeleton-related proteins as the candidates for novel signaling molecules mediating abnormal VSM contraction. In the present study, we identified the phosphorylation sites of those proteins. Furthermore, to examine if those cytoskeleton-related proteins were truly involved in abnormal VSM contraction, we developed a high throughput screening system utilizing RNAi and automated cell imaging device, and successfully narrowed down those candidate proteins.
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Report
(4 results)
Research Products
(26 results)
