Optogenetic control of macaque temporal neurons

• Project/Area Number: 22500371
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fusional basic brain science
• Research Institution: Niigata University (2011-2012); National Center of Neurology and Psychiatry (2010)
• Principal Investigator: NAKAHARA Kiyoshi 新潟大学, 研究推進機構・超域学術院, 准教授 (50372363)
• Co-Investigator(Renkei-kenkyūsha): OKADA Takashi 国立精神・神経医療研究センター, 神経研究所, 室長 (00326828)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ウイルスベクター / 光脳科学 / 皮質脳波 / 霊長類 / 長期記憶 / 内側側頭葉 / 記憶システム / 視覚野 / 線条体 / 遺伝子導入 / 連合記憶

Research Abstract

To explore the spatio-temporal dynamics of cortical activities associated with long-term memory retrieval, we aimed to combine optogenetic methods and multi-channel electrocorticogram (ECoG) recordings from the inferior and medial temporal cortices in macaque monkeys. In the first part of the project, we optogenetically stimulated the rat V1 at various stimulus frequencies, and performed spatiotemporal analysis of evoked cortical activity using a micro-ECoG array. We revealed the spatio-temporal dynamics of ECoG responses induced by optogenetic methods. In the second part of the project, we conducted multi-channel ECoG recordings from the medial temporal cortices, in two macaque monkeys while they performed a visual pair-association task. Time-frequency analyses of the ECoG responses suggested that the neural oscillation in the medial temporal lobe are involved in mechanisms of memory retrieval.

Research Products

• [Journal Article] Local and retrograde gene transfer into primate neuronal pathways via adeno-associated virus serotype 8 and 9 (2011)
  • Author(s): Masamizu Y., Okada T., Kawasaki K., Ishibashi H., Yuasa S. Takeda S., Hasegawa I. & Nakahara K
  • Journal Title: Neuroscience Volume: 193 Pages: 249-258
  • Peer Reviewed
• [Journal Article] Intrasulcal electrocorticography in macaque monkeys with minimally invasive neurosurgicalprotocols (2011)
  • Author(s): Matsuo T, Kawasaki K, Osada T, SawahataH, Suzuki T, Shibata M, Miyakawa N, Nakahara K, Iijima A, Sato N, Kawai K, Saito N, Hasegawa I
  • Journal Title: Front Syst Neurosci Volume: 5 Pages: 34-34
  • DOI: 10.3389/fnsys.2011.00034
  • Peer Reviewed
• [Journal Article] Local and retrograde gene transfer into primate neuronal pathways via adeno-associated virus serotype 8 and 9 (2011)
  • Author(s): Masamizu Y, et.al.
  • Journal Title: Neuroscience Volume: 193 Pages: 249-258
  • DOI: 10.1016/j.neuroscience.2011.06.080
  • Peer Reviewed
• [Journal Article] Efficient gene transfer into neurons in monkey brain by adeno-associated virus 8 (2010)
  • Author(s): Masamizu Y., Okada T., Ishibashi H.,Takeda S., Yuasa S. & Nakahara K
  • Journal Title: Neuroreport Volume: 21 Pages: 447-451
  • Peer Reviewed
• [Journal Article] Efficient gene transfer into neurons in monkey brain by adeno-associated virus 8. (2010)
  • Author(s): 正水芳人、岡田尚巳、石橋英俊、武田伸一、湯浅茂樹、中原潔
  • Journal Title: Neuroreport Volume: 21 Pages: 447-451
  • Peer Reviewed
• [Presentation] Cross-frequency coupling of cortical oscillations duringlong-term memory retrieval in the monkey medial temporal lobe (2013)
  • Author(s): Adachi K., Kawasaki K., Sawahata H., Matsuo T., Suzuki T., Tanigawa H., Iijima A., Hasegawa I., Nakahara K
  • Organizer: Neuro 2013
  • Place of Presentation: Kyoto
  • Year and Date: 2013-06-21
• [Presentation] Decoding recalled visual memory using electrocorticographic (ECoG) signals in macaque inferior temporal and prefrontal cortices (2013)
  • Author(s): Tanigawa H., Majima K., Sawahata H., Nakahara K., Suzuki T., Kamitani Y., Hasegawa I
  • Organizer: Neuro2013
  • Place of Presentation: Kyoto
  • Year and Date: 2013-06-21
• [Presentation] Cross-frequency coupling of cortical oscillations during long-term memory retrieval in the monkey medial temporal lobe. (2013)
  • Author(s): 中原潔
  • Organizer: Neuro2013
  • Place of Presentation: 京都国際会館（京都市）
• [Presentation] Decoding recalled visual memory using electrocorticographic (ECoG) signals in macaque inferior temporal and prefrontal cortices. (2013)
  • Author(s): 長谷川功
  • Organizer: Neuro2013
  • Place of Presentation: 京都国際会館（京都市）
• [Presentation] Spatiotemporal analysis of optogenetically evoked cortical activity using micro-ECoG array in the rat visual cortex (2012)
  • Author(s): Sato S., Nakahara K., Kawasaki K., Horie M., Sawahata H., Gougeon L., Toda H. and Hasegawa I
  • Organizer: The 22nd Annual Conference of Japanese Neural Network Society
  • Place of Presentation: Nagoya
  • Year and Date: 2012-09-12
• [Presentation] Spatiotemporal analysis of optogenetically evoked cortical activity using micro-ECoG array in the rat visual cortex. (2012)
  • Author(s): 長谷川功
  • Organizer: JNNS 2012
  • Place of Presentation: 名古屋工業大学（名古屋市）
• [Presentation] Intrasulcal electrocorticography in macaque monkeys (2011)
  • Author(s): Kawasaki K., Matsuo T., Osada T., Sawahata H., Suzuki T., Shibata M., Miyakawa N. Nakahara K., Sato N., Kawai K., Sato N. and Hasegawa I
  • Organizer: The 34th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama
• [Presentation] Intrasulcal electrocorticography in macaque monkeys. (2011)
  • Author(s): 長谷川功
  • Organizer: 第３４回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Efficient gene transfer into neurons in the primate brain via antero and etrograde infections by adeno-associated virus serotype 8 and 9. (2010)
  • Author(s): 正水芳人、岡田尚巳、川嵜圭祐、石橋英俊、武田伸一、湯浅茂樹、長谷川功、中原潔
  • Organizer: 40^<th> annual meeting of the society for neuroscience
  • Place of Presentation: San Diego Convention Center San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] Minimally invasive surgical procedures for intrasulcul electrocorticograp by in macaques. (2010)
  • Author(s): 松尾健、川嵜圭祐、澤畑博人、柴田昌宏、宮川尚久、川合謙介、佐藤昇、齋藤延人、中原潔、長谷川功
  • Organizer: 40^<th> annual meeting of the society for neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] マカクザルにおける脳溝内皮質脳波のための低侵襲手術 (2010)
  • Author(s): 松尾健、川嵜圭祐、長田貴宏、澤畑博人、宮川尚久、柴田昌宏、佐藤昇、川合謙介、中原潔、鈴木隆文、齊藤延人、宮下保司、長谷川功
  • Organizer: 社団法人日本脳神経外科学会第69回学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-27
• [Presentation] Interhemispheric functional connectivity in a subject with complete agenesis of the corpus callosum. (2010)
  • Author(s): 内田信也、中原潔、緑川晶、倉岡康治、齋藤慈子、竹本篤史、河村満、中村克樹
  • Organizer: 16^<th> annual meeting of the organization for human brain mapping
  • Place of Presentation: Palau de Congessos de Catalunya, Barcelona, Spain
  • Year and Date: 2010-06-07
• [Presentation] Efficient gene transfer into neurons in the primate brain via antero- and retrograde infections by adeno-associated virus serotype 8 and 9 (2010)
  • Author(s): Masamizu Y., Okada T., Kawasaki K., Ishibashi H., Takeda S., Yuasa S., Hasegawa I. & Nakahara K
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego, USA
• [Presentation] Minimally invasive surgical procedures for intrasulcul electrocorticography in macaques (2010)
  • Author(s): Matsuo T., Kawasaki K., Sawahata H., Shibata M., Miyakawa N., Kawai N., Sato N., Saito N., Nakahara K., Suzuki T. & Hasegawa I
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego, USA
• [Presentation] Gene transfer into the primate central nervous system via antero- and retrograde infections by adeno-associated viruses (2010)
  • Author(s): Masamizu Y., Okada T., Kawasaki K., Ishibashi H., Takeda S., Yuasa S., Hasegawa I. and Nakahara K
  • Organizer: Neuro2010
  • Place of Presentation: Kobe