Analysis of autoantibodies induced during progression to AIDS

• Project/Area Number: 22500387
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: KUWATA Takeo 熊本大学, エイズ学研究センター, 助教 (70346063)
• Co-Investigator(Renkei-kenkyūsha): IGARASHI Tatsuhiko 京都大学, ウイルス研究所, 教授 (90467431)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: HIV / SIV / アカゲザル / エイズ / モノクローナル抗体 / ウイルス / 感染症 / 免疫学

Research Abstract

The role of autoantibodies in CD4+T cell depletion during HIVinfection remains unclear. In this study, many antibodies against SIV and self antigenswere isolated by phage display method. Analysis of these antibodies revealed that theinduction of autoantibdies was not due to the cross-reactivity to SIV and suggested alow cytotoxic activity of autoantibodies in SIV infection.

Research Products

• [Journal Article] Conformational epitope consisting of theV3 and V4 loops as a target for potent andbroad neutralization of simianimmunodeficiency viruses (2013)
  • Author(s): Kuwata T, Takaki K, Yoshimura K,Enomoto I, Wu F, Ourmanov I, Hirsch VM,Yokoyama M, Sato H, Matsushita S
  • Journal Title: J Virol Volume: 87(10) Pages: 5424-36
  • Peer Reviewed
• [Journal Article] Conformational epitope consisting of the V3 and V4 loops as a target for potent and broad neutralization of simian immunodeficiency viruses. (2013)
  • Author(s): Kuwata T
  • Journal Title: J Virol. Volume: in press Issue: 10 Pages: 933-943
  • DOI: 10.1128/jvi.00201-13
  • Peer Reviewed
• [Journal Article] Isolation of potentneutralizing monoclonal antibodies froman SIV-Infected rhesus macaque by phagedisplay (2011)
  • Author(s): Kuwata T, Katsumata Y, Takaki K, MiuraT, Igarashi T
  • Journal Title: AIDS Res Hum Retroviruses Volume: 27(5) Pages: 487-500
  • Peer Reviewed
• [Journal Article] Isolation of potent neutralizing monoclonal antibodies from an SIV-Infected rhesus macaque by phage display. (2011)
  • Author(s): Kuwata, T.
  • Journal Title: AIDS Res. Hum. Retroviruses Volume: 27 Issue: 5 Pages: 487-500
  • DOI: 10.1089/aid.2010.0191
  • Peer Reviewed
• [Journal Article] Isolation of Potent Neutralizing Monoclonal Antibodies from an SIV-Infected Rhesus Macaque by Phage Display (2010)
  • Author(s): 桑田岳夫
  • Journal Title: AIDS RESEARCH AND HUMAN RETROVIRUSES Volume: Vol 26
  • Peer Reviewed
• [Presentation] gp120 のCD4-induced epitope に結合する中和抗体の解析 (2012)
  • Author(s): 田中和樹、 桑田岳夫、 丸田泰広、 園田貴丈、Kristel Ramirez 、松下修三
  • Organizer: 第26回日本エイズ学会学術集会
  • Place of Presentation: 横浜 慶應義塾大学
  • Year and Date: 2012-11-26
• [Presentation] SIV 感染サルから分離された中和抗体 B404 は V3,V4ループを含む Env 立体構造を認識する (2012)
  • Author(s): 桑田岳夫、吉村和久、松下修三
  • Organizer: 第26回日本エイズ学会学術集会
  • Place of Presentation: 横浜慶應義塾大学
  • Year and Date: 2012-11-24
• [Presentation] CRF01_AE HIV-1 感染症例IgG の交差中和活性の解析 (2012)
  • Author(s): 園田貴丈、Samatchaya Boonchawalit、田中和樹、丸田康広、Kristel Ramirez、桑田岳夫、松下修三
  • Organizer: 第26回日本エイズ学会学術集会
  • Place of Presentation: 横浜慶應義塾大学
  • Year and Date: 2012-11-24
• [Presentation] Conformational epitope inbolving V3 and V4 loops is a major tartget for antibody-mediated neutralization in SIV smH635-infected macaques. (2012)
  • Author(s): Kuwata T.
  • Organizer: 13th Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: 阿蘇グランヴィリオホテル、熊本県阿蘇市
• [Presentation] SIV感染サルから分離された中和抗体B404はV3,V4ループを含むEnv立体構造を認識する (2012)
  • Author(s): 桑田岳夫
  • Organizer: 第26回日本エイズ学会学術集会・総会
  • Place of Presentation: 慶應義塾大学日吉キャンパス、横浜市
• [Presentation] SIVsmH635FC感染サルにおける特定のエピトープと遺伝子に偏った中和抗体の誘導 (2011)
  • Author(s): 桑田岳夫
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京(東京都)
  • Year and Date: 2011-12-02
• [Presentation] Biased induction of neutralizing antibodies with particular specificity and gene usage in SIVsmH635FC-infected macaques (2011)
  • Author(s): 桑田岳夫
  • Organizer: 12th Kumamoto AIDS Seminar
  • Place of Presentation: 阿蘇リゾートグランヴィリオホテル(熊本県)
  • Year and Date: 2011-10-21
• [Presentation] Isolation of Potent Neutralizing Monoclonal Antibodies against V3 Loop from SIV-infected Macaques (2011)
  • Author(s): 桑田岳夫
  • Organizer: AIDS Vaccine 2011
  • Place of Presentation: Bangkok Convention Centre (Bangkok, Thai)
  • Year and Date: 2011-09-12
• [Presentation] ファーシ・ティスプレイ法による強力な抗SIV中和活性をもつモノクローナル抗体の作製 (2010)
  • Author(s): 桑田岳夫
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島県)
  • Year and Date: 2010-11-09
• [Presentation] Generation of monoclonal antibodies with potent neutralizing activity from a SIV-infected macaque (2010)
  • Author(s): 桑田岳夫
  • Organizer: The 28th Annual Symposium for Nonhuman Primate Models for AIDS
  • Place of Presentation: Westin New Orleans Canal Place (New Orleans, USA)
  • Year and Date: 2010-10-19
• [Presentation] Isolation of monoclonal neutralizing antibodies from SIV-infected macaques by phage display (2010)
  • Author(s): 桑田岳夫
  • Organizer: 11th Kumamoto AIDS Seminar
  • Place of Presentation: 阿蘇リゾートグランヴィリオホテル(熊本県)
  • Year and Date: 2010-10-07
• [Presentation] Contribution of antibody response to effective viral suppression and progression of disvease during SIV infection (2010)
  • Author(s): 桑田岳夫
  • Organizer: International Young Investigator Symposium
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-04-04
• [Remarks]
  • URL: http://sendou.kuma-u.jp/index.html