Behavior of Bone Marrow-derived Cells Following in vivo Transplantation

• Project/Area Number: 22500388
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Nagoya City University
• Principal Investigator: TAGUCHI Osamu 名古屋市立大学, 大学院・医学研究科, 研究員 (00142167)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 実験動物技術 / 骨髄細胞 / 臓器再生 / 間質上皮相互作用 / マウス / 移植 / 骨髄移植 / 放射線照射 / 再生 / 間質細胞 / 上皮 / 上皮・間質相互作用 / GFP / scid / x線照射 / FACS / CD45

Research Abstract

Following injection of green fluorescent protein positive (GFP^+) bone marrow (BM) cells into lethally irradiated wild-type mice, the organs of the recipient mice (BM transplantation [BMT] mice) were regenerated . The maintenance of epithelial structure and function is controlled stromal cells. In BMT mice, GFP^+ stromal cells were regenerated fairly rapidly indicating that BM-derived stromal cells play a pivotal role in epithelial renewal and are crucial for maintaining organ structure and function. BM-derived cells in the periphery possess a special “key” to return to the BM and then to migrate to various organs to become resident cells.

Research Products

• [Journal Article] Behavior of Bone Marrow-Derived Cells Following in Vivo Transplantation: Differentiation into Stromal Cells with Roles in Organ Maintenance. (2013)
  • Author(s): Taguchi O, Tsujimura K, Kontani K, Harada Y, Nomura S, Ikeda H, Morita A, Sugiura H, Hayashi N, Yatabe Y, Seto M, Tatematsu M, Takahashi T, Fukushima A.
  • Journal Title: Am J Pathol. Volume: 182 Pages: 1255-1262
• [Journal Article] Identification of keratocyte-like cells differentiated from circulating bone marrow-derived cells in the mouse cornea. (2013)
  • Author(s): Harada Y, Ishida W, Fukuda K, Sumi T, Kawakita T, Taguchi O, Fukushima A.
  • Journal Title: Med Mol Morphol. Volume: (in press)
• [Journal Article] Oral administration of Ag suppresses Ag-induced allergic conjunctivitis in mice: critical timing and dose of Ag. (2013)
  • Author(s): Ishida W.
  • Journal Title: Br J Ophthalmol. Volume: 97 Pages: 492-497
  • Peer Reviewed
• [Journal Article] Behavior of bone marrow-derived cells following in vivo transplantation: differentiation into stromal cells with roles in organ maintenance. (2013)
  • Author(s): Taguchi O.
  • Journal Title: Am J Pathol. Volume: 182 Pages: 1255-1262
  • Peer Reviewed
• [Journal Article] Antitumor activity of an interleukin-2 monoclonal antibody in a murine osteosarcoma transplantation model. (2012)
  • Author(s): Kohyama K.
  • Journal Title: Anticancer Res. Volume: 32 Pages: 779-782
  • Peer Reviewed
• [Journal Article] Zoledronate sensitizes neuroblastoma-derived tumor-initiating cells to cytolysis mediated by human γδ T cells. (2012)
  • Author(s): Nishio N.
  • Journal Title: J Immunother. Volume: 35 Pages: 598-606
  • Peer Reviewed
• [Journal Article] Antitumor activity of an interleukin-2 monoclonal antibody in a murine osteosarcoma transplantation model (2012)
  • Author(s): Kohyama K, Sugiura H, Kozawa E, Wasa J, Yamada K, Nishioka A, Kamei Y, Taguchi O
  • Journal Title: Anticancer Res Volume: 32 Pages: 779-782
  • Peer Reviewed
• [Journal Article] The Tumor Suppressive Effect of Angiotensin II Type 1 Receptor Antagonist in a Murine Osteosarcoma Model (2011)
  • Author(s): Wasa J, Sugiura H, Kozawa E, Kohyama K, Yamada K, Taguchi O
  • Journal Title: Anticancer Res Volume: 31 Pages: 123-127
  • Peer Reviewed
• [Journal Article] Promotion and maintenance of leukemia by ERG (2011)
  • Author(s): Tsuzuki S, Taguchi O, Seto M
  • Journal Title: Blood Volume: 117 Pages: 3858-3868
  • Peer Reviewed
• [Journal Article] Synergistic effect of Bcl2, Myc and Ccnd1 transforms mouse primary B-cells into malignant cells (2011)
  • Author(s): Nakagawa M, Tsuzuki S, Honma K, Taguchi O, Seto M
  • Journal Title: Haematologica Volume: 96 Pages: 1318-1326
  • Peer Reviewed
• [Journal Article] Cellular Imaging Using Equivalent Cross-Relaxation Rate Technique in Rabbit VX-2 Tumor Model (2011)
  • Author(s): Nishiofuku H, Matsushima S, Taguchi O, Inaba Y, Yamaura H, Sato Y, Tanaka T, Kichikawa K
  • Journal Title: Cancer Inform Volume: 10 Pages: 227-232
  • Peer Reviewed
• [Journal Article] Promotion and maintenance of leukemia by ERG. (2011)
  • Author(s): Tsuzuki S, Taguchi O, Seto M.
  • Journal Title: Blood Volume: (In press)
  • Peer Reviewed
• [Journal Article] Suppression of tumour metastasis in a murine osteosarcoma model with anti-CD25 monoclonal antibody treatment. (2011)
  • Author(s): Wasa J, Sugiura H, Kozawa E, Kohyama K, Yamada K, Taguchi O.
  • Journal Title: Anticancer Res. Volume: 30 Pages: 123-127
  • Peer Reviewed
• [Journal Article] Suppression of tumour metastasis in a murine osteosarcoma model with anti-CD25 monoclonal antibody treatment. (2011)
  • Author(s): Kozawa E, Sugiura H, Wasa J, Kohyama K, Yamada K, Nishioka A, Nishida Y, Ishiguro N, Taguchi O.
  • Journal Title: Anticancer Res. Volume: 30 Pages: 5019-5022
  • Peer Reviewed