The mechanism of loss of Erc gene ameliorates renal carcinogenesis and modulates integrin related pathway

• Project/Area Number: 22500389
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Juntendo University
• Principal Investigator: ZHANG Danqing 順天堂大学, 医学部, 助教 (40296877)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Toshiyuki 順天堂大学, 医学部, 准教授 (40260070) ; HINO Okio 順天堂大学, 医学部, 教授 (90127910)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Erc/mesothelin / Tsc2遺伝子 / GPIアンカー蛋白 / ノックアウトマウス / シグナル伝達

Research Abstract

To investigate the position of Erc/mesothelin in signal pathway, we continue the studies and found that a novel function for Erc in the regulation of cell phenotype through controlling EGF receptor phosphorylation (Tyr1092), integrin β1 recycling and localization to lipid raft via a specific downstream effector pathway.

Research Products

• [Journal Article] Tuberin activates and controls the distribution of Rac1 viaassociation with p62 and ubiquitinthrough the mTORC1 signaling pathway (2013)
  • Author(s): Okura H, Kobayashi T, Zhang D, Hino O, et al
  • Journal Title: Int J Oncol Volume: 2 Pages: 5-8
  • Peer Reviewed
• [Journal Article] Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA (2013)
  • Author(s): Kawai A, Kobayashi T, Hino O
  • Journal Title: Int J Oncol Volume: 42(5) Issue: 5 Pages: 1597-604
  • DOI: 10.3892/ijo.2013.1862
  • Peer Reviewed
• [Journal Article] TSC1 controls distribution of actin fibers through its effect on function of Rho family of small GTPases and regulates cell migration and polarity (2013)
  • Author(s): Ohsawa M, Kobayashi T, Hino O, et al
  • Journal Title: PLoS One Volume: 8(1) Issue: 1 Pages: e54503-e54503
  • DOI: 10.1371/journal.pone.0054503
  • Peer Reviewed
• [Journal Article] Fenton reaction induced cancer in wild type rats recapitulates genomic alterations observed in human cancer. (2012)
  • Author(s): Liu Y, Izumiya M, Abe K, Ochiai M, Jiang L, Nagai H, Okazaki Y, Murakami H, Sekido Y, Arai E, Kanai Y, Hino O, Takahashi T, Nakagama H, Toyokuni S
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: e43403-e43403
  • DOI: 10.1371/journal.pone.0043403
  • Peer Reviewed
• [Journal Article] Deficiency of the Erc/mesothelin gene ameliorates renal carcinogenesis in Tsc2 knockout mice (2011)
  • Author(s): Zhang D, Kobayashi T, Hino O, et al
  • Journal Title: Cancer Sci Volume: 102(4) Issue: 4 Pages: 720-7
  • DOI: 10.1111/j.1349-7006.2011.01846.x
  • NAID: 10029291977
  • Peer Reviewed
• [Journal Article] Deficiency of the Erc/mesothelin gene ameliorates renal cazcinogenesis in Tsc2 knockout mice. (2011)
  • Author(s): Zhang D, et al.
  • Journal Title: Cancer Science Volume: (Epubに掲載)
  • Peer Reviewed
• [Journal Article] Serine 62 is a phosphorylation site in folliculin, the Birt-Hogg-Dubegene product (2010)
  • Author(s): Wang L, Kobayashi T, Zhang D, Hino O, et al
  • Journal Title: FEBS Lett Volume: 584(1) Issue: 1 Pages: 39-43
  • DOI: 10.1016/j.febslet.2009.11.033
  • Peer Reviewed
• [Journal Article] Age dependence of radiation-induced renal cell carcinomas in an Eker rat model (2010)
  • Author(s): T. Kokubo, S. Kakinuma, M. Nishimura, Y. Shimada, et. Al
  • Journal Title: Cancer Science Volume: 101(3) Issue: 3 Pages: 616-623
  • DOI: 10.1111/j.1349-7006.2009.01456.x
  • NAID: 10026589138
  • Peer Reviewed
• [Journal Article] Phosphorylated hamartin-Hsp70 complex regulates apoptosis via mitochondrial localization (2010)
  • Author(s): Inoue H, Hino O, Kobayashi T, et al
  • Journal Title: iochem Biophys Res Commun Volume: 391(1) Issue: 1 Pages: 1148-53
  • DOI: 10.1016/j.bbrc.2009.12.054
  • Peer Reviewed
• [Journal Article] Mesothelin (MSLN) promoter is hypomethylated in malignant mesothelioma, but its expression is not associated with methylation status of the promoter (2010)
  • Author(s): Tan K, Hino O, Fujii H, et al
  • Journal Title: Hum Pathol Volume: 41(9) Issue: 9 Pages: 1330-8
  • DOI: 10.1016/j.humpath.2010.03.002
  • Peer Reviewed
• [Journal Article] Suppression of cell death by the secretory form of N-terminal ERC/mesothelin (2010)
  • Author(s): Wang T, Kajino K, Hino O, et al
  • Journal Title: Int J Mol Med Volume: 26(2) Pages: 185-91
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20596597
  • Peer Reviewed
• [Journal Article] Antitumor activity of anti-C-ERC/mesothelin monoclonal antibody in vivo (2010)
  • Author(s): Inami K, Abe M, Hino O, et al
  • Journal Title: Cancer Sci Volume: 101(4) Issue: 4 Pages: 969-74
  • DOI: 10.1111/j.1349-7006.2009.01463.x
  • NAID: 10026591120
  • Peer Reviewed
• [Journal Article] Mesothelin (MSLN) promoter is hypomethylated in malignant mesothelioma, but its expression is not associated with methylation status of the promoter. (2010)
  • Author(s): Tan K, et al.
  • Journal Title: Human Pathology Volume: 41 Pages: 1330-8
  • Peer Reviewed
• [Journal Article] Antitumor activity of anti-C-ERC/mesothelin monoclonal antibody in vivo. (2010)
  • Author(s): Inami K, et al.
  • Journal Title: Cancer Science Volume: 101 Pages: 969-74
  • Peer Reviewed
• [Presentation] Reduced the phosphorylation of epidermal growth factor (EGF) receptor in Erc-deficient renal tumor cells of Tsc2 KO mice. (2012)
  • Author(s): 小橋（張）丹青，ら
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌，札幌
• [Presentation] Loss of Erc/mesothelin gene reduced the phosphorylation of EGFR and modulates PI3K-Akt pathway in renal tumor cells of Tsc2 KO mice. (2012)
  • Author(s): 小橋（張）丹青，ら
  • Organizer: 第26回モロシヌス研究会
  • Place of Presentation: 東京大学弥生講堂，東京
• [Presentation] TSC2 positively regulates activity and controls intracellular localization of Rac1 through mTORC1 signaling pathway (2011)
  • Author(s): Okura H, et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-16
• [Presentation] Loss of Erc/mesothelin gene decreased the growth of renal tumors in Tsc2 KO mice and modulates PI3K-Akt pathway (2011)
  • Author(s): Zhang D, et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-14
• [Presentation] Enhancement of cell adhesion by Erc/mesothelin through modulation of PI3K-Akt pathway in Tsc2 KO mice renal tumor cells (2011)
  • Author(s): Zhang D, et al
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-03
• [Presentation] Loss of Erc/mesothelin gene decreased the growth of renal tumors in Tsc2 knockout mice. (2010)
  • Author(s): Zhang D, et al.
  • Organizer: The 4th AFLAS Congress Meeting
  • Place of Presentation: 台北国際会議中心(台湾)
  • Year and Date: 2010-11-09
• [Presentation] Erc/mesothelin gene regulates the growth of renal tumors in Tsc2 KO mice and modulates integrin pathway. (2010)
  • Author(s): Zhang D, et al.
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-22
• [Presentation] Loss of Erc gene ameliorates renal carcinogenesis and modulates integrin path-way in Tsc2 KO mouse. (2010)
  • Author(s): Zhang D, et al.
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザ(東京)
  • Year and Date: 2010-04-27
• [Remarks] ホームページ
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/bunshi_byori/index.html