Analysis of the central respiratory disorder in PACAP-deficient mice
| Project/Area Number |
22500390
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Showa University |
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Principal Investigator |
ARATA Satoru 昭和大学, 遺伝子組換え実験室, 准教授 (20159502)
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| Co-Investigator(Kenkyū-buntansha) |
ONIMARU Hiroshi 昭和大学, 医学部, 准教授 (30177258)
NAKAMACHI Tomoya 昭和大学, 遺伝子組換え実験室, 助教 (30433840)
畠 あずさ 昭和大学, 動物実験施設, 技術員 (20568290)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 乳幼児突然死症候群(SIDS) / 呼吸中枢障害マウスモデル / Pituitary adenylate cyclase activating polypeptide(PACAP) / SIDS / PACAP / 呼吸中枢 / 低酸素受容体 / 中枢性呼吸疾患 / 疾患モデルマウス |
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| Research Abstract |
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide expressed widely in nervous tissues. PACAP knockout (-/-) mice display a sudden infant death syndrome (SIDS)-like phenotype, although the underlying physiological mechanism to explain this remains unclear. Here, we reporton the presence of abnormal respiratory activity in PACAP -/- mice under hypoxic conditions, which provides a basis for the SIDS-like phenotype. PACAP -/- mice display a lowered baseline respiratory activity compared with wild-type animals, and an abnormal response to hypoxia. Histological and real time PCR analyses indicated that the catecholaminergic system in the medulla was impaired in PACAP-/- mice, suggesting that endogenous PACAP affects respiratory centers in the medulla via its action on the catecholaminergic system. We propose that disruption of this system may beinvolved in the sudden infant death-like phenotype of PACAP -/- mice. Thus, disorders of the catecholaminergic system involved with O_2sensing could be implicated in underlying neuronal mechanisms responsible for SIDS.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] IL-6 and PACAP receptor expression and localization after global brain ischemia in mice2012
Author(s)
Nakamachi T, Tsuchida M, Kagami N, Yofu S, Wada Y, Hori M, Tsuchikawa D, Yoshikawa A, Imai N, Nakamura K, ArataS, Shioda S
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Journal Title
J Mol Neurosci
Volume: 48(3)
Pages: 518-25
Related Report
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[Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates proliferation of reactive astrocytes in vitro2011
Author(s)
Nakamachi T, Nakamura K, Oshida K, Kagami N, Mori H, Watanabe J, Arata S, Yofu S, Endo K, Wada Y, Hori M, Tsuchikawa D, Kato M, Shioda S
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Journal Title
J Mol Neurosci
Volume: 43
Pages: 16-21
Related Report
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[Journal Article] Dietary cholesterol reduces plasma triacylglycerol in apolipoprotein E-null mice: suppression of lipin-1 and -2 in the glycerol-3-phosphate pathway2011
Author(s)
Obama T, Nagaoka S, Akagi K, Kato R, Horiuchi N, Horai Y, Aiuchi T, ArataS, Yamaguchi T, Watanabe M, Itabe H
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Journal Title
Related Report
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[Journal Article] m-Calpain induction in vascular endothelial cells on human and mouse atheromas and its roles in VE-cadherin disorganization and atherosclerosis2011
Author(s)
Miyazaki T, Taketomi Y, Takimoto M, Lei XF, Arita S, Kim-Kaneyama JR, ArataS, Ohata H, Ota H, Murakami M, Miyazaki A
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Journal Title
Circulation
Volume: 124 (23)
Pages: 2522-32
Related Report
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[Journal Article] m-Calpain is induced in vascular endothelial cells on human atheroma and accelerates atherosclerotic lesion development through proteolytic disorganization of VE-cadherin in hypercholesterolemic mice2011
Author(s)
Miyazaki, T., Taketomi, Y., Takimoto, M., Lei, X., Akita, S., Kim-Kaneyama, J., Arata, S., Ohata, H., Ota, H., Murakami, M., and Miyazaki, A.
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Journal Title
Circulation
Volume: 124
Issue: 23
Pages: 2522-2532
DOI
Related Report
Peer Reviewed
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