Identification of hematopoietic stem cell and establishment of BLT mouse of common marmoset
| Project/Area Number |
22500391
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Erika 公益財団法人実験動物中央研究所, マーモセット研究部, 部長 (70390739)
SUZUKI Ryuji 国立病院機構・相模原病院, 床研究センター, 教授 (70373470)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | マーモセット / 造血幹細胞 / 免疫不全マウス |
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| Research Abstract |
The development of human hematopoieitc system is well documented by using xeno-transplantation system. However, as the reconstitution of human hematopoietic system in xeno- environment is incomplete, the repopulation ability may be low-estimated. A new system using non-human primate may be a better choice to clarify the development pathway of primate hematopoiesis. Calithrix jucchus, is a candidate of an experimental animal that bears 2-3 animals in one pregnancy and twice per year. We purified CD117+ cell and CD34+ cell fractions and transplanted into immuno-deficient NOG mice. After 8 weeks, CD117+ cells engrafted more efficiently than CD117- cells in bone marrow and spleen of NOG mice and CD34 expression was not enough to keep repopulation activity in xeno-transplantation. CD34+CD117+ cells had similar repopulation ability compared to CD117 single-positive cells as evaluated by marmoset CD45 expressing cell ratio. The cells derived from both CD117+CD34+ and CD117+CD34- fractions developed into myeloid lineage cells and lymphoid cells 12 weeks after the transplantation. The differentiation ability was higher in CD117+CD34+ cells than in CD117 single positive cells. NOG bone marrow cells included CD34+CD117+ cells 4 weeks after the transplantation. These results suggest that multipotential hematopoietic stem cells are involved in CD117+ cell fraction.
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] Immune-Related Gene Expression Profile in Laboratory Common Marmosets Assessed by an Accurate Quantitative Real-Time PCR Using Selected Reference Genes2013
Author(s)
Fujii Y., Kitaura K., Matsutani T., Shirai K., Suzuki S., Takasaki T., Kumagai K., Kametani Y.,Shiina T., Takabayashi S., Katoh H., Hamada Y., Kurane I., Suzuki R
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A new method for quantitative analysis of the T cell receptor V region repertoires in healthy common marmosets by microplate hybridization assay2012
Author(s)
Kitaura K, Fujii Y, Matsutani T, Shirai K, Suzuki S, Takasaki T, Shimada S, Kametani Y, Shiina T, Takabayashi S, Katoh H, Ogasawara K, Kurane I, Suzuki R
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Journal Title
J Immunol Methods
Volume: 384
Pages: 81-91
Related Report
Peer Reviewed
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[Journal Article] Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Calithrix Jucchus (common marmoset)2012
Author(s)
NunomuraS, ShimadaS, Kametani Y*, YamadaY, YoshiokaM, Suemizu H, Ozawa M, Itoh T, Kono A, Suzuki R, Tani K, Ando K, Yagita H, Ra C, Habu S, Satake M, Sasaki E
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Journal Title
Int. Immunol
Volume: 24
Pages: 593-603
Related Report
Peer Reviewed
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[Journal Article] Anti-tumor Effect of New HER2 Peptide Vaccination Based on B Cell Epitope2011
Author(s)
H. Miyako H, Kametani Y*, Katano I, ItoR, Tsuda B, FurukawaA, SaitoY, IshikawaD, Ogino K, SasakiS, ImaiK, Habu S, MakuuchiH and Tokuda Y
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Journal Title
Anticancer Res
Volume: 31
Pages: 3361-3368
Related Report
Peer Reviewed
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[Journal Article] A novel ENU-induced mutation in Phospholipase C gamma 2 causes inflammatory arthritis, metabolic defects,and in vitro male infertility in the mouse2011
Author(s)
Abe K, Fuchs H, Boersma A, Hans W, Yu P, Kalaydjiev S, Klaften M, Adler T, Calzada-Wack J, Mossbrugger I, Rathkolb B, Rozman J, Prehn C, Maraslioglu M, Kametani Y, Shimada S, Adamski J, Busch DH, Esposito I, Klingenspor M, Wolf E, Wurst W, Gailus-Durner V
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Journal Title
de Angelis MH. Arthritis Rheum
Volume: 63
Pages: 1301-11
Related Report
Peer Reviewed
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[Journal Article] Protective potential of Bacillus coagulans, a spore-forming lactic acid bacterium, against cytomegalovirus infection2010
Author(s)
K.Shimizu, Y.Koga, Yuji Aiba,Kiyoshi Kobayashi, Akihiro Hata, Hiroki Ishikawa, Y.Kametani, Kazuo Tanaka, Shiro Ono S
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Journal Title
Noda Report Jpn. Assoc. Biol. Func. Rec
Volume: 13
Pages: 34-36
Related Report
Peer Reviewed
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