| Project/Area Number |
22500391
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Erika 公益財団法人実験動物中央研究所, マーモセット研究部, 部長 (70390739)
SUZUKI Ryuji 国立病院機構・相模原病院, 床研究センター, 教授 (70373470)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | マーモセット / 造血幹細胞 / 免疫不全マウス |
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| Research Abstract |
The development of human hematopoieitc system is well documented by using xeno-transplantation system. However, as the reconstitution of human hematopoietic system in xeno- environment is incomplete, the repopulation ability may be low-estimated. A new system using non-human primate may be a better choice to clarify the development pathway of primate hematopoiesis. Calithrix jucchus, is a candidate of an experimental animal that bears 2-3 animals in one pregnancy and twice per year. We purified CD117+ cell and CD34+ cell fractions and transplanted into immuno-deficient NOG mice. After 8 weeks, CD117+ cells engrafted more efficiently than CD117- cells in bone marrow and spleen of NOG mice and CD34 expression was not enough to keep repopulation activity in xeno-transplantation. CD34+CD117+ cells had similar repopulation ability compared to CD117 single-positive cells as evaluated by marmoset CD45 expressing cell ratio. The cells derived from both CD117+CD34+ and CD117+CD34- fractions developed into myeloid lineage cells and lymphoid cells 12 weeks after the transplantation. The differentiation ability was higher in CD117+CD34+ cells than in CD117 single positive cells. NOG bone marrow cells included CD34+CD117+ cells 4 weeks after the transplantation. These results suggest that multipotential hematopoietic stem cells are involved in CD117+ cell fraction.
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