Analysis of the inflammatory mechanisms caused by the Ali18 mutation in molecular and cellular levels

• Project/Area Number: 22500392
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokai University
• Principal Investigator: ABE Koichiro 東海大学, 医学部, 講師 (90294123)
• Co-Investigator(Renkei-kenkyūsha): TAKAMATSU Nobuhiko 北里大学, 理学部, 教授 (40206876) ; ITO Michihiko 北里大学, 理学部, 准教授 (90240994) ; KIMURA Minoru 東海大学, 医学部, 教授 (10146706)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 疾患モデル / 実験動物学 / 病態モデル / 自己炎症性症候群 / 関節炎 / シグナル伝達 / リン酸化 / マウス遺伝学 / 炎症 / ENU

Research Abstract

The Ali18 mutation in an kinase gene causes inflammatory arthritis in mice. However, its molecular mechanisms remains largely unknown. Here we show that Ali18; W/Wv double mutant mice show no inflammatory arthritis. These results indicate the effects of the Ali18 mutation on mast cells trigger the initiation of inflammation.

Research Products

• [Journal Article] A Cre Knock-in Mouse Line on the Sickle Tail Locus Induces Recombination in the Notochord and Intervertebral Discs. (2012)
  • Author(s): Abe, K. et al.
  • Journal Title: Genesis Volume: 50 Issue: 10 Pages: 758-765
  • DOI: 10.1002/dvg.22035
  • Peer Reviewed
• [Journal Article] Hrabe de Angelis M. A novel N-ethyl-N-nitrosourea-induced mutation in phospholipase Cg2 causes inflammatory arthritis, metabolic defects, and male fertility in vitro in a murine model (2011)
  • Author(s): 阿部幸一郎, Fuchs H, Boersma A, Hans W, Yu P, Kalaydjiev, Klaften M, Adler T, Calazada-Wack, Mossbrugger I, Rathkolb B, Rozman J, Prehn C, Maraslioglu M, Kametani Y, Shimada S, Adamski J, Busch DH, Esposito I, Kingenspor M, Wolf E, Wurst W, Gailus-Durner V, Ka
  • Journal Title: Arthritis & Rheumatism Volume: 63 Issue: 5 Pages: 1301-1311
  • DOI: 10.1002/art.30280
  • Peer Reviewed
• [Journal Article] The relationship between Wnt/b-catenin and TGF-b/BMP signals in the intervertebral disc cell (2011)
  • Author(s): Hiyama A, Skai D, 阿部幸一郎, Mochida J
  • Journal Title: Journal of Cellular Physiology Volume: 226 Issue: 5 Pages: 1139-1148
  • DOI: 10.1002/jcp.22438
  • Peer Reviewed
• [Journal Article] The relationship between the Wnt/β-catenin and TGF-β/BMP signals in the intervertebral disc cell (2011)
  • Author(s): Hiyama A., Sakai D, Tanaka M, Arai F, Nakajima D, Abe K, Mochida J(7人中6番目)
  • Journal Title: Journal of Cellular Physiology Volume: 226 Pages: 1139-1148
  • Peer Reviewed
• [Journal Article] A novel N-ethyl-N-nitrosourea-induced mutation in Phospholipase Cγ2 causes inflammatory arthritis, metabolic defects, and male infertility in vitro in a murine model (2011)
  • Author(s): Abe K, Fuchs H, Boersoma A, et al., (28人中1番目)
  • Journal Title: Arthritis & Rheumatism Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] The relationship between the Wnt/β-catenin and TGF-β/BMP signals in the intervertebral disc cell (2011)
  • Author(s): Hiyama A.Sakai D, Tanaka M, Arai F, Nakajima D, Abe K, Mochida J, (7人中6番目)
  • Journal Title: Journal of Cellular Physiology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Enhancement of intervertebral disc cell senescence by Wnt/b-catenin signaling-induced matrix (2010)
  • Author(s): Hiyama A, Sakai D, Risbud MV, Tanaka M, Arai F, 阿部幸一郎, Mochida J
  • Journal Title: Arthritis & Rheumatism Volume: 62 Issue: 10 Pages: 3036-3047
  • DOI: 10.1002/art.27599
  • Peer Reviewed
• [Journal Article] Enhancement of intervertebral disc cell senescence by Wnt/β-catenin signaling-induced matrix metalloproteinase expression (2010)
  • Author(s): Hiyama A.Sakai D, Risbud MV, Tanaka M, Arai F, Abe K, Mochida J, (7人中6番目)
  • Journal Title: Arthritis & Rheumatism Volume: 62 Pages: 3036-3047
  • Peer Reviewed
• [Presentation] Sktcreマウス系統を用いた脊柱椎間板におけるコンディショナルノックアウトシステムの構築 (2012)
  • Author(s): 阿部幸一郎,荒木喜美,谷河麻耶,仙波圭,安藤卓,佐藤正宏,酒井大輔,檜山明彦,持田讓治,山村研一
  • Organizer: 日本実験動物科学・技術九州2012
  • Place of Presentation: 別府
  • Year and Date: 2012-05-24
• [Presentation] レチノイド投与による炎症性関節炎モデルマウスへの薬理作用 (2012)
  • Author(s): 阿部幸一郎,谷河麻耶,内藤佳津子,石川久美子,谷口真沙美
  • Organizer: 日本実験動物科学・技術 九州2012
  • Place of Presentation: 別府
  • Year and Date: 2012-05-24
• [Presentation] Sktcreマウス系統を用いた椎間板コンディショナルノックアウトシステムの構築 (2012)
  • Author(s): 阿部幸一郎
  • Organizer: 第59回日本実験動物学会総会
  • Place of Presentation: 別府国際コンベンションセンター
• [Presentation] レチノイド投与による炎症性関節炎モデルマウスへの薬理効果 (2012)
  • Author(s): 阿部幸一郎
  • Organizer: 第46回日本実験動物技術者協会総会
  • Place of Presentation: 別府国際コンベンションセンター
• [Presentation] Ali14変異マウスを用いた受精におけるホスホリパーゼCγ2の機能解析 (2011)
  • Author(s): 荻原早苗、Auke Boersma, 本杉奈美、中村健司、Susan Marschall, Martin Hrabe de Angelis, 木村穣、阿部幸一郎
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2011-05-27
• [Presentation] Intervertebral disc-specific expression of Cre recombinase in Sktcreknock-in mice (2011)
  • Author(s): 阿部幸一郎, Araki K, Tanigawa M, Semba K, Imai K, Yamamura K-I
  • Organizer: Cartilage Biology & Pathology (Gordon Research Conference)
  • Place of Presentation: Ventura, California, USA
  • Year and Date: 2011-03-09
• [Presentation] ホスホリパーゼCγ2 の新規機能亢進型変異であるAli14はマウスにおいて炎症性関節炎を引き起こす (2010)
  • Author(s): 阿部幸一郎, Fuchs H, Boersma A, Yu P, Kalaydjiev S, Klaften M, Adler T, Hans W, Rathkolb B, Prehn C, Adamski J, Busch D, Wolf E, Gailus-Druner, Katan M, Marschall S, Wagner S, Hrabe de Angelis M
  • Organizer: 日本実験動物学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-12
• [Presentation] ホスホリパーゼCg2新規機能亢進型突然変異であるAli14はマウスにおいて炎症性関節炎を引き起こす (2010)
  • Author(s): 阿部幸一郎、Fuchs H, Boersma A, ら, (18人中1番目)
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都テルサ(京都)
  • Year and Date: 2010-05-12
• [Remarks] 東海大学 医学部 阿部幸一郎研究室
  • URL: http://abe.med.u-tokai.ac.jp/index.html
• [Remarks] 東海大学 TWAVE
  • URL: http://www.u-tokai.ac.jp/tweve/volume02_2/index.html
• [Remarks] 東海大学 TWAVE_PDF
  • URL: http://www.u-tokai.ac.jp/international/about/twave.html
• [Remarks] 東海大学 医学部 阿部幸一郎研究室
  • URL: http://abe.med.u-tokai.ac.jp/index.html
• [Remarks] 東海大学 TWAVE
  • URL: http://www.u-tokai.ac.jp/tweve/volume02_2/index.html
• [Remarks] 東海大学 TWAVE_PDF
  • URL: http://www.u-tokai.ac.jp/international/about/twave.html
• [Remarks]
  • URL: http://abe.med.u-tokai.ac.jp/index.html
• [Remarks]
  • URL: http://abe.med.u-tokai.ac.jp/index.html
• [Patent(Industrial Property Rights)] 炎症性疾患又は骨粗鬆症のスクリーニング方法 (2010)
  • Inventor(s): 阿部幸一郎、マーティン フラベデ アンゲリス、ヘルムット フックス
  • Industrial Property Rights Holder: 学校法人東海大学
  • Filing Date: 2010-07-23
• [Patent(Industrial Property Rights)] 炎症性疾患又は骨粗鬆症の治療剤のスクリーニング方法 (2010)
  • Inventor(s): 阿部幸一郎, (他2名)
  • Industrial Property Rights Holder: 東海大学
  • Industrial Property Number: 2010-165551
  • Filing Date: 2010-07-23