Genetic dissection of brown adipose tissue function using mouse inter-subspecific consomic strains

• Project/Area Number: 22500395
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: National Institute of Genetics
• Principal Investigator: TAKADA Toyoyuki 国立遺伝学研究所, 系統生物研究センター, 助教 (20356257)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: マウス / コンソミック系統 / 褐色脂肪 / エネルギー代謝 / 遺伝子間相互作用 / 褐色脂肪組織

Research Abstract

In this study, I intend to uncover the genetic determinants that control the age-associated changes of energy metabolism-associated brown adipose tissue function by functional genomics with mouse inter-subspecific consomic strains. Using B6-ChrNMSM consomic strain panel and B6-ChrNMSM-derived congenic strains, I conducted systematic phenotype screening of energy metabolism related physiological traits by developing measurements for new parameters of the energy metabolism-related phenotypes such as fat deposition. Currently, I am exploring causative genome difference responsible for high calorie diet-dependent fat-deposition.

Research Products

• [Journal Article] Chromosome substitution strains: gene discovery, functional analysis, and systems studies (2012)
  • Author(s): Nadeau, J. H., Forejt, J., Takada, T., Shiroishi, T.
  • Journal Title: Mammalian genome Volume: 23 Pages: 9-10
  • Peer Reviewed
• [Journal Article] Complex quantitative traits cracked by the mouse inter-subspecific consomic strains (2012)
  • Author(s): Takada, T., Shiroishi, T.
  • Journal Title: Experimental animals Volume: 61(4) Pages: 375-388
  • NAID: 10030800301
  • Peer Reviewed
• [Journal Article] Genetic divergence and the architecture of complex traits in chromosome substitution strains of mice (2012)
  • Author(s): Spiezio, S. H., Takada, T., Shiroishi, T. &Nadeau J. H
  • Journal Title: BMC genetics Volume: 13(1), Pages: 38-38
  • Peer Reviewed
• [Journal Article] Mouse models for atopic dermatitis developed in Japan (2012)
  • Author(s): Yonekawa, H., Takada, T., Shitara, H., Taya, C., Matsushima, Y., Matsuoka, K., Kikkawa, Y
  • Journal Title: Atopic Dermatitis-Disease Etiology and Clinical Management, edt, Jorge Esparza-Gordillo Pages: 21-38
  • DOI: 10.5772/26084
  • ISBN: 9789535101109
  • Peer Reviewed
• [Journal Article] Chromosome substitution strains: gene discovery, functional analysis, and systems studies. (2012)
  • Author(s): Nadeau, J. H.
  • Journal Title: Mammalian genome Volume: 23 Issue: 9-10 Pages: 693-705
  • DOI: 10.1007/s00335-012-9426-y
  • Peer Reviewed
• [Journal Article] Complex quantitative traits cracked by the mouse inter-subspecific consomic strains. (2012)
  • Author(s): Takada, T.
  • Journal Title: Experimental animals Volume: 61 Pages: 375-388
  • NAID: 10030800301
  • Peer Reviewed
• [Journal Article] Genetic divergence and the genetic architecture of complex traits in chromosome substitution strains of mice. (2012)
  • Author(s): Spiezio, S.
  • Journal Title: BMC genetics Volume: 13 Issue: 1 Pages: 38-38
  • DOI: 10.1186/1471-2156-13-38
  • Peer Reviewed
• [Journal Article] 次世代シーケンサを利用したモデル生物マウスのゲノム機能解析 (2012)
  • Author(s): 高田豊行
  • Journal Title: 遺伝 Volume: 66 Pages: 333-337
• [Journal Article] Complex quantitative traits cracked by the mouse inter-subspecific consomic strains (2012)
  • Author(s): Takada T.
  • Journal Title: Experimental Animals Volume: (In press)
  • NAID: 10030800301
  • Peer Reviewed
• [Journal Article] マウス表現型関連データベースの紹介,疾患 (2011)
  • Author(s): 高田豊行
  • Journal Title: モデルマウス表現型解析指南
• [Journal Article] 肥満の遺伝学 (2011)
  • Author(s): 高田豊行
  • Journal Title: ありふれた病気の遺伝的素因解明に向けて Volume: 65 Pages: 5-6
• [Journal Article] マウス表現型関連データベースの紹介 (2011)
  • Author(s): 高田豊行
  • Journal Title: 疾患モデルマウス表現型解析指南(編集,山村研一,若菜茂晴) Pages: 360-372
• [Journal Article] 肥満の遺伝学-ありふれた病気の遺伝的素因解明に向けて.遺伝 (2011)
  • Author(s): 高田豊行
  • Journal Title: 遺伝 Volume: 65 Pages: 5-6
• [Journal Article] An Atopic Dermatitis-Like Skin Disease with Hyper-IgE-Emia Develops in Mice Carrying a Spontaneous Recessive Point Mutation in the Traf3ip2 (Act1/CIKS) Gene (2010)
  • Author(s): Matsushima Y., Kikkawa Y., Takada T., Matsuoka K., Seki Y., Yoshida H., Minegishi Y., Karasuyama H. And Yonekawa H.:
  • Journal Title: The Journal of Immunology Volume: 185 Pages: 2340-2349
  • Peer Reviewed
• [Journal Article] An Atopic Dermatitis-Like Skin Disease with Hyper-IgE-Emia Develops in Mice Carrying a Spontaneous Recessive Point Mutation in the Traf3ip2 (Act1/CIKS) Gene. (2010)
  • Author(s): Matsushima Y.
  • Journal Title: The Journal of Immunology Volume: 185 Pages: 2340-2349
  • Peer Reviewed
• [Journal Article] The ancestor of extant Japanese fancy mice contributed to the mosaic genomes of classical inbred strains
  • Author(s): Takada, T., Ebata, T., Noguchi, H., Keane, T., Adams, D., Narita, T., Shin-I, T., Fujisawa, H., Toyoda, A., Abe, K., Obata, Y.,Sakaki, Y., Moriwaki, K., Fujiyama, A., Kohara, Y., Shiroishi, T.
  • Journal Title: Genome Res Volume: 1
  • Peer Reviewed
• [Presentation] Ancestry of extant Japanese fancy mice contributed to the mosaic genomes of classical inbred strains (2012)
  • Author(s): Takada, T., Ebata, T., Noguchi, H.,Keane, K., Wong, K., Adams, D., Narita, T.,Shin-I, T., Fujisawa, H., Toyoda, A., Abe,K., Obata, Y., Sakaki, Y., Moriwaki, K.,Fujiyama, A., Kohara, Y. and Shiroishi, T.
  • Organizer: 26th International Mammalian Genome Conference
  • Place of Presentation: Florida, USA.
• [Presentation] 日本産野生由来マウス系統MSM/Ms を用いた加齢性脂肪蓄積の機能ゲノム解析 (2012)
  • Author(s): 高田豊行, 三田晃彦, 米川博通, 森脇和郎, 城石俊彦
  • Organizer: 第59回日本実験動物学会総会,
  • Place of Presentation: 別府.
• [Presentation] Ancestry of extant Japanese fancy mice contributed to the mosaic genomes of classical inbred strains. (2012)
  • Author(s): Takada, T.
  • Organizer: 26th International Mammalian Genome Conference
  • Place of Presentation: Florida, USA
• [Presentation] 日本産野生由来マウス系統MSM/Msを用いた加齢性脂肪蓄積の機能ゲノム解析. (2012)
  • Author(s): 高田豊行
  • Organizer: 第59回日本実験動物学会総会
  • Place of Presentation: 別府
• [Presentation] Functional genomics powered by mouse inter-subspecific genome diversity (2011)
  • Author(s): Takada, T
  • Organizer: Mouse Genetics Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-10-27
• [Presentation] Functional genomics powered by mouse inter-subspecific genome diversity (2011)
  • Author(s): Takada T.
  • Organizer: Mouse Genetics Symposium, Seoul National University
  • Place of Presentation: Seoul (Korea)(招待講演)
  • Year and Date: 2011-10-27
• [Presentation] 日本産野生由来マウス系統MSM/Ms を用いた加齢性エネルギー代謝の機能ゲノム解析 (2011)
  • Author(s): 高田豊行, 三田晃彦, 森脇和郎, 米川博通, 城石俊彦
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都.
• [Presentation] MSM/Ms 系統を基盤にしたマウス機能ゲノム学データベースの構築, (2011)
  • Author(s): 高田豊行
  • Organizer: 日本実験動物学会総会・ワークショップ「国内実験動物データベースの現状と展望」第58回日本実験動物学会総会,
  • Place of Presentation: 東京
• [Presentation] 日本産野生由来マウス系統MSM/Msを用いた加齢性エネルギー代謝の機能ゲノム解析 (2011)
  • Author(s): 高田豊行
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都
• [Presentation] MSM/Ms系統を基盤にしたマウス機能ゲノム学データベースの構築,日本実験動物学会総会・ワークショップ「国内実験動物データベースの現状と展望」 (2011)
  • Author(s): 高田豊行
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京
• [Presentation] Age-associated change of energy metabolism are genetically disected by mouse inter-subspecific consomic strains (2010)
  • Author(s): Takada, T, Mita, A., Wakana, S., Moriwaki, K., Yonekawa, H., Shiroishi, T
  • Organizer: 23th International Mammalian Genome Conference
  • Place of Presentation: Crete, Greece.
• [Presentation] マウス亜種間の表現型多様性とゲノム多型性に基づいた機能ゲノム学のためのデータベース (2010)
  • Author(s): 高田豊行
  • Organizer: 日本遺伝学会第82回大会・ワークショップ「モデル動物における表現型解析の標準化と情報統合
  • Place of Presentation: 札幌.
• [Presentation] 多因子形質解析とその実験モデルマウスの開発 (2010)
  • Author(s): 高田豊行
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都.「受賞講演」
• [Presentation] マウス亜種間コンソミック系統群による加齢性エネルギー代謝表現型の遺伝解析 (2010)
  • Author(s): 高田豊行, 三田晃彦, 森脇和郎, 米川博通, 城石俊彦
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都
• [Presentation] 多因子形質解析とその実験モデルマウスの開発 (2010)
  • Author(s): 高田豊行
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都(招待講演)
• [Presentation] マウスMSM/Ms系統のゲノム解析 (2010)
  • Author(s): 高田豊行
  • Organizer: 第24回モロシヌス研究会
  • Place of Presentation: 阿蘇
• [Presentation] マウス亜種間の表現型多様性とゲノム多型性に基づいた機能ゲノム学のためのデータベース (2010)
  • Author(s): 高田豊行
  • Organizer: 日本遺伝学会
  • Place of Presentation: 札幌
• [Presentation] Age-associated change of energy metabolism are genetically disected by mouse inter-subspecific consomic strains (2010)
  • Author(s): Takada T.
  • Organizer: 23th International Mammalian Genome Conference
  • Place of Presentation: Crete, Greece
• [Book] Atopic Dermatitis - Disease Etiology and Clinical Management (2012)
  • Author(s): Yonekawa, H.
  • Total Pages: 17
  • Publisher: Mouse models for atopic dermatitis developed in Japan.