Analysis of relationship between age-dependent phenotype change and gene

• Project/Area Number: 22500396
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TAKAHASHI Eiki 独立行政法人理化学研究所, 動物資源開発支援ユニット, 支援ユニットリーダー (40446521)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 老化 / 表現型解析 / マウス / 行動解析 / QTL解析 / 運動量 / 記憶能力 / アルコール嗜好性 / 老化促進モデルマウス / 記憶 / 体外受精 / 加齢依存的表現型

Research Abstract

In this study to examine the relationship between aging and behavioral changes, we used senescence-accelerated mouse (SAM) strain, behavioral tests including motor activity, memory, and alcohol preference tests, and QTL analyses. We have found chromosomal regions involved in age-dependent phenotype changes in the behavioral test. We are trying to identify the causative gene(s) using the database and other mutant strains.

Research Products

• [Journal Article] Analysis of ischemic neuronal injury in the Cav2.1 channel α_1 subunit mutant mice (2013)
  • Author(s): Tian X, Zhou Y, Gao L, He G, Jiang W, Li W, Takahashi E
  • Journal Title: Biochem Biophys Res Commun Volume: 434 Issue: 1 Pages: 60-64
  • DOI: 10.1016/j.bbrc.2013.03.066
  • Peer Reviewed
• [Journal Article] Study of the timing of Caesarean hysterectomy and progesterone administration for routine microbiological decontamination of mice (2013)
  • Author(s): Niimi K, Ito N, Nishioka C, Arasaki T, Watanabe K, Tsuji A, Arai T, Takahashi E
  • Journal Title: Research Animals Volume: 1 Pages: 13-17
  • Peer Reviewed
• [Journal Article] New ataxic tottering-6j mouse allele containing a Cacna1a gene mutation (2012)
  • Author(s): Li W, Zhou Y, Tian X, Kim TY, Ito N, Watanabe K, Tsuji A, Aoyama Y, Niimi K, Arai T, Takahashi E
  • Journal Title: PLoS ONE Volume: 7 Issue: 8 Pages: e44230-e44230
  • DOI: 10.1371/journal.pone.0044230
  • Peer Reviewed
• [Journal Article] Ca^<2+> channel currents in dorsal root ganglion neurons of P/Q-type voltage-gated Ca^<2+> channel mutant mouse, rolling mouse Nagoya (2012)
  • Author(s): Fukumoto N, Kitamura N, Niimi K, Takahashi E, Itakura C, Shibuya I
  • Journal Title: Neurosci Res Volume: 73 Issue: 3 Pages: 199-206
  • DOI: 10.1016/j.neures.2012.04.006
  • Peer Reviewed
• [Journal Article] Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation (2012)
  • Author(s): X.-S. Yue
  • Journal Title: PLoS ONE Volume: 7 Issue: 3 Pages: e32707-e32707
  • DOI: 10.1371/journal.pone.0032707
  • Peer Reviewed
• [Journal Article] Comparative anatomy of marmoset and mouse cortex from genomic expression. (2012)
  • Author(s): Mashiko H, Yoshida AC, Kikuchi SS, Niimi K, Takahashi E, Aruga J, Okano H, Shimogori T.
  • Journal Title: J Neurosci. Volume: 32 Issue: 15 Pages: 5039-5053
  • DOI: 10.1523/jneurosci.4788-11.2012
  • Peer Reviewed
• [Journal Article] Emotional behavior in heterozygous rolling mouse Nagoya Cav2.1 channel mutant mice (2011)
  • Author(s): Takahashi E, Niimi K, Itakura C
  • Journal Title: Neurobiol Aging Volume: 32 Issue: 3 Pages: 486-496
  • DOI: 10.1016/j.neurobiolaging.2009.03.001
  • Peer Reviewed
• [Journal Article] Role of Cav2.1-mediated NMDA receptor signaling at nucleus accumbens in spatial short-term memory (2011)
  • Author(s): Takahashi E, Niimi K, Itakura C
  • Journal Title: Behav Brain Res Volume: 218 Issue: 2 Pages: 353-356
  • DOI: 10.1016/j.bbr.2010.12.019
  • Peer Reviewed
• [Journal Article] Impairment of neuropsychological behaviors in ganglioside GM3-knockout mice (2011)
  • Author(s): Niimi K, et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 406 Pages: 24-528
  • Peer Reviewed
• [Journal Article] Subthreshold pharmacological and genetic approaches to analyzing Cav2.1-mediated NMDA receptor signaling in short-term memory (2010)
  • Author(s): Takahashi E, Niimi K, Itakura C
  • Journal Title: Eur J Pharmacol Volume: 64 Pages: 113-118
  • Peer Reviewed
• [Journal Article] Levetiracetam-mediated emotional behavior in heterozygous Rolling Nagoya Cav2.1 channel mutant mice (2010)
  • Author(s): Takahashi E, Niimi K, Itakura C
  • Journal Title: Pharmacol Biochem Behav Volume: 96 Issue: 3 Pages: 294-300
  • DOI: 10.1016/j.pbb.2010.05.020
  • Peer Reviewed
• [Journal Article] Impairment of spatial short-term memory following acute administration of the NMDA receptor antagonist in heterozygous rolling Nagoya mice carrying the Cav2.1α1 mutation (2010)
  • Author(s): Takahashi E, Niimi K, Itakura C
  • Journal Title: Behav Brain Res Volume: 213 Issue: 1 Pages: 121-125
  • DOI: 10.1016/j.bbr.2010.04.030
  • Peer Reviewed
• [Journal Article] Interaction between Cav2.1α_1 and CaMKII in Cav2.1α_1 mutant mice, Rolling Nagoya (2010)
  • Author(s): Takahashi E, Niimi K, Itakura C
  • Journal Title: J Mol Neurosci Volume: 41 Issue: 2 Pages: 223-229
  • DOI: 10.1007/s12031-009-9216-5
  • Peer Reviewed
• [Journal Article] Neonatal motor functions in Cacna1a-mutant rolling Nagoya mice (2010)
  • Author(s): Takahashi E, Niimi K, Itakura C
  • Journal Title: Behav Brain Res Volume: 207 Pages: 273-279
  • Peer Reviewed
• [Journal Article] Hallucinogenic 5-Hydroxytryptamine 2A receptor agonist effects in senescence accelerated mouse (2010)
  • Author(s): Niimi K, Takahashi E, Itakura C
  • Journal Title: Exp Anim Volume: 59 Pages: 441-447
  • Peer Reviewed
• [Journal Article] Subthreshold pharmacological and genetic approaches to analyzing Cav2.1-mediated NMDA receptor signaling in short-term memory. (2010)
  • Author(s): Eiki Takahashi
  • Journal Title: Eur J Pharmacol Volume: 645 Pages: 113-118
  • Peer Reviewed
• [Journal Article] Hallucinogenic 5-hydroxytryptamine 2A receptor agonist effects in senescence-accelerated mice. (2010)
  • Author(s): Kimie Niimi
  • Journal Title: Exp Anim Volume: 59 Pages: 441-417
  • Peer Reviewed
• [Journal Article] Levetiracetam-mediated emotional behavior in heterozygous rolling Nagoya Cav2.1 channel mutant mice. (2010)
  • Author(s): Eiki Takahashi
  • Journal Title: Pharmacol Biochem Behav Volume: 96 Pages: 294-300
  • Peer Reviewed
• [Journal Article] Impairment of spatial short-term memory following acute administration of the NMDA receptor antagonist in heterozygous rolling Nagoya mice carrying the Cav2.1 alphal mutation. (2010)
  • Author(s): Eiki Takahashi
  • Journal Title: Behav Brain Res Volume: 213 Pages: 121-125
  • Peer Reviewed
• [Journal Article] Behavioral and neurochemical characterization of mice deficient in the N-type calcium channel alpha1B subunit. (2010)
  • Author(s): Osamu Nagkagawasai
  • Journal Title: Behav Brain Res Volume: 208 Pages: 224-230
  • Peer Reviewed
• [Journal Article] Neonatal motor functions in Cacna1 a-mutant rolling Nagoya mice. (2010)
  • Author(s): Eiki Takahashi
  • Journal Title: Behav Brain Res Volume: 207 Pages: 273-279
  • Peer Reviewed
• [Journal Article] Interaction between Cav2.1 alpha1 and CaMKII in Cav2.1 alpha1 mutant mice, Rolling Nagoya. (2010)
  • Author(s): Eiki Takahashi
  • Journal Title: J Mol Neurosci Volume: 41 Pages: 223-229
  • Peer Reviewed
• [Presentation] Senescence-Accelerated Mouse(SAM)P1マウスの運動機能障害に関する検討 (2013)
  • Author(s): 青山曜、金泰延、新美君枝、高橋英機、板倉智敏
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-29
• [Presentation] enescence-Accelerated Mouse（SAM）P1マウスの運動機能障害に関する検討. (2013)
  • Author(s): 青山曜、金泰延、新美君枝、高橋英機、板倉智敏.
  • Organizer: 第155 回日本獣医学会
  • Place of Presentation: 東京都 東京大学
• [Presentation] Senescence-Accelerated Mouse(SAM)P1マウスの運動機能の検討 (2012)
  • Author(s): 青山曜、新美君枝、高橋英機、板倉智敏
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手
  • Year and Date: 2012-09-14
• [Presentation] 老化促進マウスSAMP6系統と他の近交系マウス系統における運動量およびドーパミンシステムの加齢変化の比較 (2012)
  • Author(s): 新美君枝、高橋英機、板倉智敏
  • Organizer: 第59回日本実験動物学会
  • Place of Presentation: 大分
  • Year and Date: 2012-05-26
• [Presentation] 網羅的表現型解析システムを用いた脳機能研究モデルマウスの開発と記憶のメカニズム解明に関する研究 (2012)
  • Author(s): 高橋英機
  • Organizer: 第153回日本獣医学会
  • Place of Presentation: 大宮
  • Year and Date: 2012-03-28
• [Presentation] Generation and analysis of animal models in Brain Science (2012)
  • Author(s): Eiki Takahashi
  • Organizer: Inauguration lecture-Advisory Professor Eiki Takahashi
  • Place of Presentation: Shanghai Jiao Tong University Shanghai, China(招待講演)
  • Year and Date: 2012-02-04
• [Presentation] 老化促進マウスSAMP6系統と他の近交系マウス系統における運動量およびドーパミンシステムの加齢変化の比較. (2012)
  • Author(s): 新美君枝、高橋英機、板倉智敏.
  • Organizer: 第59回日本実験動物学会
  • Place of Presentation: 大分県 別府国際コンベンションセンター
• [Presentation] Senescence-Accelerated Mouse（SAM）P1マウスの運動機能の検討. (2012)
  • Author(s): 青山曜、新美君枝、高橋英機、板倉智敏.
  • Organizer: 第154 回日本獣医学会
  • Place of Presentation: 岩手県 岩手大学
• [Presentation] Senescence-Accelerated Mouse (SAM) P6およびP8を用いたアルコール嗜好性の検討 (2011)
  • Author(s): 新美君枝、高橋英機、板倉智敏
  • Organizer: 第152回日本獣医学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-19
• [Presentation] Senescence-Accelerated Mouse (SAM) P6およびP8を用いたアルコール嗜好性の検討 (2011)
  • Author(s): 新美君枝, ら
  • Organizer: 第152回日本獣医学会
  • Place of Presentation: 大阪府立大学(大阪府)
  • Year and Date: 2011-09-19
• [Presentation] レベチラセタムを投与したCav2.1α1変異マウスの脳高次機能に関する解析 (2011)
  • Author(s): 高橋英機, ら
  • Organizer: 第152回日本獣医学会
  • Place of Presentation: 大阪府立大学(大阪府)
  • Year and Date: 2011-09-19
• [Presentation] A role of Laboratory Animals in Brain Science Research (2011)
  • Author(s): Eiki Takahashi
  • Organizer: Korean Association of Laboratory Animal Science (KALAS) International Symposium 2011
  • Place of Presentation: Muju-gun, Jeonbuk, Korea(招待講演)
  • Year and Date: 2011-08-25
• [Presentation] 創薬研究に不可欠な病態モデル動物 (2011)
  • Author(s): 高橋英機
  • Organizer: 東北大学農学部体と健康セミナー
  • Place of Presentation: 東北大学(宮城県)(招待講演)
  • Year and Date: 2011-05-10
• [Presentation] Combined genetic and pharmacological approaches for cognitive function. (2010)
  • Author(s): Eiki Takahashi
  • Organizer: 第4回アジア実験動物学会連合
  • Place of Presentation: 中華民国台北市
  • Year and Date: 2010-11-09
• [Presentation] 霊長類実験動物マーモセットへの期待と理化学研究所脳科学総合研究センター新実験動物飼育行動解析棟の紹介 (2010)
  • Author(s): 高橋英機
  • Organizer: 第62回富山大学生命科学先端研究センター学術セミナー
  • Place of Presentation: 富山県富山市
  • Year and Date: 2010-10-01
• [Presentation] Cav2.1変異マウスを用いた短期記憶に関する行動薬理学的解析 (2010)
  • Author(s): 高橋英機
  • Organizer: 第150回日本獣医学会
  • Place of Presentation: 北海道帯広市
  • Year and Date: 2010-09-17
• [Presentation] 老化促進マウスSAMP6系統における運動量とドーパミンシステムの検討 (2010)
  • Author(s): 新美君枝、高橋英機、板倉智敏
  • Organizer: 第57回日本実験動物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-13
• [Presentation] 老化促進マウスSMMP6系統における運動量とドーパミンシステムの検討 (2010)
  • Author(s): 新美君枝
  • Organizer: 第57回日本実験動物学会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2010-05-13
• [Book] Effect of aging on changes of nociception and Cav2τ1 expression patterns in seven inbred mouse strains. Neurological Disorders: New Research (ISBN 978-1-62257-042-3) (2012)
  • Author(s): Takahashi E
  • Publisher: Thomas CE and Moore JR. Nova Science Publishers, Inc. New York
• [Book] Cav2.1-mediated NMDA receptor signaling in short-term memory. Short-Term Memory: New Research (ISBN 978-1-62100-860-6) (2012)
  • Author(s): Takahashi E
  • Publisher: Kalivas G and Petralia SF. Nova Science Publishers, Inc. New York
• [Book] Cav2.1 channelopathy and mouse genetic approaches to investigate function and dysfunction of Cav2.1 channel. Calcium Signaling (ISBN 978-1-61324-313-8) (2012)
  • Author(s): Takahashi E.
  • Publisher: Yamaguchi M. Nova Science Publishers, Inc. New York
• [Book] Neurological Disorders: New Research (2012)
  • Author(s): Eiki Takahashi
  • Total Pages: 12
  • Publisher: Nova Science Publishers, Inc.
• [Remarks]
  • URL: http://www.brain.riken.jp/jp/faculty/details/59