Development of quantum dot-based multimodal contrast agents for whole body imaging

• Project/Area Number: 22500407
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The Institute of Physical and Chemical Research (2011-2012); Osaka University (2010)
• Principal Investigator: JIN Takashi 独立行政法人理化学研究所, ナノバイオプローブ研究チーム, ナノバイオプローブ研究チーム (80206367)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 生体イメージング / マルチモーダル造影剤 / 量子ドット / 近赤外蛍光 / MRI / ヒト乳癌 / ヌードマウス / バイオイメージング / マルチモーダル / 造影剤 / 蛍光 / 近赤外

Research Abstract

The objective of this work was to develop multimodal contrast agents based on near-infrared (NIR) emitting quantum dots for whole body imaging. We could synthesis highly fluorescent near infrared emitting CdSeTe/CdS quantum dots with fluorescence quantum yield of > 50 %. Furthermore, we developed multimodal (NIR/MRI) contrast agents consisting of CdSeTe/CdS quantum dots and Fe_3O_4 nanoparticles and conducted dualmodal imaging of human breast tumors implanted to a nude mouse.

Research Products

• [Journal Article] Matrix Metalloproteinase-9 Triggers the Gap Junction Impairment and Somatosensory Neuronal Dysfunction in Septic Encephalopathy (2013)
  • Author(s): Huan Wang, Yukio Imamura, Naoya Matsumoto, Nao Yoshikawa, Junichiro Nakagawa, Kazuma Yamakawa, Tomoki Yamada, Yuki Murakami, Satoko Mitani, Takashi Jin, Hiroshi Ogura, Takeshi Shimazu and Akitoshi Seiyama
  • Journal Title: Biochem Volume: Pharmacol.2 Issue: 01 Pages: 108-108
  • DOI: 10.4172/2167-0501.1000108
  • Peer Reviewed
• [Journal Article] Synthesis of green-emitting Pt8 nanoclusters for biomedical imaging by pre-equilibrated Pt/PAMAM(G4-OH) and mild condition (2013)
  • Author(s): S. Tanaka, K. Aoki, A. Muramatsu, H. Ishitobi, T. Jin and Y. Inouye
  • Journal Title: Opt. Mater. Exp Volume: 3 Pages: 157-165
  • Peer Reviewed
• [Journal Article] Bioluminescence resonance energy transfer coupled near-infrared QDs using GST-tagged luciferase for in vivo imaging (2013)
  • Author(s): Miyuki Hasegawa, Tsukasaki Yoshikazu, Tatsuya Ohyanagi and Takashi Jin
  • Journal Title: Chem. Commun Volume: 49 Pages: 228-230
  • Peer Reviewed
• [Journal Article] Multilayered, Core/shell Nanoprobes Based on Magnetic Ferric Oxide Particles and Quantum Dots for Multimodality Imaging of Breast Cancer Tumors (2012)
  • Author(s): Qiang Ma, Yuko Nakane, Yuki Mori, Miyuki Hasegawa, Yoshichika Yoshioka, Tomonobu M. Watanabe, Kohsuke Gonda, Noriaki Ohuchi and Takashi Jin
  • Journal Title: Biomaterilas Volume: 33 Pages: 8486-8494
  • Peer Reviewed
• [Journal Article] Bovine serum albumin-coated quantum dots as a cytoplasmic viscosity probe in a single living cell (2012)
  • Author(s): Y. Nakane, S. Sasaki, M. Kinjo, and T. Jin
  • Journal Title: Anal. Methods Volume: 4 (7) Pages: 1903-1905
  • Peer Reviewed
• [Journal Article] Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod-tagged myosin V (2012)
  • Author(s): M, Ohmachi, Y. Komori, A. H. Iwane, F. Fujii, T. Jin, and T. Yanagida
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 5294-5298
  • Peer Reviewed
• [Journal Article] Histochemical analyses and quantum dot imaging of microvascular blood flow with pulmonary edema in living mouse lungs by "in vivo cryotechnique (2012)
  • Author(s): Yurika Saitoh, Nobuo Terada, Sei Saitoh, Nobuhiko Ohno, Takashi Jin, Shinichi, Ohno
  • Journal Title: Histochemistry and Cell Biology Volume: 137(2) Pages: 137-51
  • Peer Reviewed
• [Journal Article] Bovine serum albumin-coated quantum dots as a cytoplasmic viscosity probe in a single living cell (2012)
  • Author(s): Y. Nakane
  • Journal Title: Anal. Methods Volume: 4 Pages: 1903-1905
  • Peer Reviewed
• [Journal Article] Histochemical analyses and quantum dot imaging of microvascular blood flow with pulmonary edema in living mouse lungs by "in vivo cryotechnique" (2012)
  • Author(s): Yurika Saitoh, Nobuo Terada, Sei Saitoh, Nobuhiko Ohno, Takashi Jin, Shinichi, Ohno
  • Journal Title: Histochemistry and Cell Biology Volume: 137 Pages: 137-151
  • Peer Reviewed
• [Journal Article] Importance of sialic acid residues illuminated by live animal imaging using phosphorylcholine self-assembled monolayers-coated quantum dots (2011)
  • Author(s): Ohyanagi, Tatsuya; Nagahori, Noriko; Shimawaki, Ken; Yamashita, Tadashi; Hinou, Hiroshi; Sasaki, Akira; Jin, Takashi; Kinjo, Masataka; Nishimura, Shin-Ichiro
  • Journal Title: J. Am. Chem. Soc Volume: 133 Pages: 12507-12517
  • Peer Reviewed
• [Journal Article] Non-radiative exciton recombination through excitation energy transfer in quantum dot clusters (2011)
  • Author(s): J. Miyazaki, S. Kinoshita, and T. Jin
  • Journal Title: J. Lumin Volume: 131 Pages: 539-542
  • Peer Reviewed
• [Journal Article] Bio-distribution and toxicity assessment of intravenously injected anti-HER2 antibody conjugated CdSe quantum dots in Wistar rat (2011)
  • Author(s): Dhermendar, K. Tiawari, Takashi Jin, and J Behari
  • Journal Title: Int. J. Nanomed Volume: 6 Pages: 463-475
  • Peer Reviewed
• [Journal Article] Fluorescent platinium (Pt5) nanoclusters: their synthesis, purification, characterization, and application to bio-imaging (2011)
  • Author(s): Shin-Ichi Tanaka, Jun Miyazaki, Dhermendar, K. Tiawari, Takashi Jin, and Yasushi Inouye
  • Journal Title: Angew. Chem. Int. Ed Volume: 50 Pages: 431-435
  • Peer Reviewed
• [Journal Article] Dose-dependent in vivo-toxicity assessment of silver nanoparticles in wistar rat (2011)
  • Author(s): Dhermendar, K. Tiawari, Takashi Jin, and J Behari
  • Journal Title: Toxicology Mechanism and Methods Volume: 21 Pages: 13-24
  • Peer Reviewed
• [Journal Article] Analysis of excitation energy transfer in quantum dot clusters in the presence of nonluminescent dots (2011)
  • Author(s): Jun Miyazaki, Shuichi Kinoshita, Takashi Jin
  • Journal Title: Phys. Status Solidi Volume: C 8, No. 1 Pages: 54-57
  • Peer Reviewed
• [Journal Article] Importance of sialic acid residues illuminated by live animal imaging using phosphorylcholine self-assembled monolayers-coated quantum dots (2011)
  • Author(s): Ohyanagi, Tatsuya; Nagahori, Noriko; Shimawaki, Ken; Yamashita, Tadashi; Hinou, Hiroshi; Sasaki, Akira; Jin, Takashi; Kinjo, Masataka; Nishimura, Shin-Ichiro
  • Journal Title: J.Am.Chem.Soc. Volume: 133 Pages: 12507-12517
  • Peer Reviewed
• [Journal Article] Non-radiative exciton recombination through excitation energy transfer in quantum dot clusters (2011)
  • Author(s): J.Miyazaki, S.Kinoshita, T.Jin
  • Journal Title: J.Lumin. Volume: 131 Pages: 539-542
  • Peer Reviewed
• [Journal Article] Bio-distribution and toxicity assessment of intravenously injected anti-HER2 antibody conjugated CdSe quantum dots in Wistar rat (2011)
  • Author(s): Dhermendar, K.Tiawari, Takashi Jin, J Behari
  • Journal Title: Int.J.Nanomed. Volume: 6 Pages: 463-475
  • Peer Reviewed
• [Journal Article] Fluorescent platinium (Pt_5) nanoclusters : their synthesis, purification, characterization, and application to bio-imaging (2011)
  • Author(s): Shin-Ichi Tanaka, Jun Miyazaki, Dhermendar, K.Tiawari, Takashi Jin, Yasushi Inouye
  • Journal Title: Angew.Chem.Int.Ed. Volume: 50 Pages: 431-435
  • Peer Reviewed
• [Journal Article] Dose-dpendent in vivo-toxicity assessment of silver nanoparticles in wistar rat (2011)
  • Author(s): Dhermendar, K.Tiawari, Takashi Jin, J Behari
  • Journal Title: Toxicology Mechanism and Methods Volume: 21 Pages: 13-24
  • Peer Reviewed
• [Journal Article] Visualization of microvascular blood flow in mouse kidney and spleen by quantum dot injection with "in vivo cryotechnique (2010)
  • Author(s): Nobuo Terada, Yurika Saitoh, Sei Saitoh, Nobuhiko Ohno, Takashi Jin, and Shinichi Ohno
  • Journal Title: Microvasc Res Volume: 80 Pages: 419-498
  • Peer Reviewed
• [Journal Article] Antibody-ProteinA conjugated quantum dots for multiplexed imaging of surface receptors in living cells (2010)
  • Author(s): Takashi Jin, Dhermendra K. Tiwari, Shin-ichi Tanaka, Yasushi Inouye, Keiko Yoshizawa and Tomonobu M. Watanabe
  • Journal Title: Molecular. BioSystems Volume: 6 Pages: 2325-2332
  • Peer Reviewed
• [Journal Article] Real-time Nanoscopy by using Blinking Enhanced Quantum Dots (2010)
  • Author(s): Tomonobu M. Watanabe, Shingo, Fukui, Takashi Jin, Fumihiko Fujii, and Toshio Yanagida
  • Journal Title: Biophys Volume: J. 99
  • Peer Reviewed
• [Journal Article] A quantum dot-based ratiometric pH sensor (2010)
  • Author(s): Takashi Jin, Akira Sasaki, Masataka Kinjo, and Jun Miyazaki
  • Journal Title: Chem. Commun Volume: 46 Pages: 2408-2410
  • Peer Reviewed
• [Journal Article] Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex of rhodamine 800 and p-sulfonatocalix[8]arene (2010)
  • Author(s): Takashi Jin
  • Journal Title: Sensors Volume: 10 Pages: 2438-2449
  • Peer Reviewed
• [Journal Article] Synthesis and surface modification of fluorescent semiconductor nanoparticles, and their use for biomedical applications (2010)
  • Author(s): Takashi Jin
  • Journal Title: J. Soc. Power Technol. Japan Volume: 47 Pages: 646-655
  • NAID: 10026665510
  • Peer Reviewed
• [Journal Article] A quantum dot-based ratiometric pH sensor (2010)
  • Author(s): Takashi Jin, Akira Sasaki, Masataka Kinjo, Jun Miyazaki
  • Journal Title: Chem.Commun. Volume: 46 Pages: 2408-2410
  • Peer Reviewed
• [Journal Article] Real-time Nanoscopy by using Blinking Enhanced Quantum Dots (2010)
  • Author(s): Tomonobu M.Watanabe, Shingo, Fukui, Takashi Jin, Fumihiko Fujii, Toshio Yanagida
  • Journal Title: Biophys.J. Volume: 99
  • Peer Reviewed
• [Journal Article] Antibody-ProteinA conjugated quantum dots for multiplexed imaging of surface receptors in living cells (2010)
  • Author(s): Takashi Jin, Dhermendra K.Tiwari, Shin-ichi Tanaka, Yasushi Inouye, Keiko Yoshizawa, Tomonobu M.Watanabe
  • Journal Title: Molecular.BioSystems Volume: 6 Pages: 2325-2332
  • Peer Reviewed
• [Journal Article] Dose-dependent in vivo-toxicity assessment of silver nanoparticles in wistar rat (2010)
  • Author(s): Dhermendra, K.Tiawari, Takashi Jin, J Behari
  • Journal Title: Toxicology Mechanism and Methods Volume: 21 Pages: 13-24
  • Peer Reviewed
• [Journal Article] 蛍光性半導体ナノ粒子の合成、表面修飾とその医療応用 (2010)
  • Author(s): 神隆
  • Journal Title: 粉体工学会誌 Volume: 47 Pages: 646-655
  • Peer Reviewed
• [Book] 蛍光性半導体量ナノ粒子の合成と医療への応用 (2012)
  • Author(s): 大柳達也、神 隆
  • Publisher: ケミカルエンジニアリング、10月号
• [Book] Neuroscience - Dealing with Frontiers Chap 7 Neurochemistry in the pathophysiology of septic encephalopathy (2011)
  • Author(s): Yukio Imamura, Huan Wang, Naoya Matsumoto, Hiroshi Ogura, Takeshi Shimazu, Takashi Jin and Akitoshi Seiyama
  • Publisher: In-Tech open access publisher
• [Book] 「生産と技術」半導体量子ドット、その合成法と生命科学への応用 (2011)
  • Author(s): 神 隆
  • Publisher: 大阪大学生産技術研究会 第63巻,第2号,
• [Book] 蛍光性半導体ナノ粒子(量子ドット)の合成、表面修飾とその医療応用 (2010)
  • Author(s): 神 隆
  • Publisher: 粉体工学会誌、 vol47
• [Remarks]
  • URL: http://www.riken.go.jp/research/labs/qbic/cell_dyn/nanobio_probes/
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/research/lab/qbic/nanobio/index.html
• [Remarks]
  • URL: http://www.ifrec.osaka-u.ac.jp/jpn/laboratory/nano-biomaterials/outline.php