Involvement of Polycomb group genes in cell line property of human ES and iPS cells

• Project/Area Number: 22500425
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Shinshu University
• Principal Investigator: TOMOTSUNE Daihachiro 信州大学, 医学部, 助教 (80283802)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Katsunori 信州大学, 医学部, 教授 (30170666) ; YUE Fengming 信州大学, 医学部, 助教 (20532865)
• Research Collaborator: MOGI Akimi 信州大学, 医学系研究科, 大学院生
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ヒト ES 細胞 / ヒトiPS 細胞 / ポリコーム / 多能性幹細胞 / 未分化維持機構 / ヒトiPS細胞 / ヒトES細胞 / 未分化破綻 / ポリコーム遺伝子 / ES細胞 / iPS細胞 / 分化傾向 / 未分化維持

Research Abstract

In this study, we produced the spontaneous differentiation, and then analyzed global changes of gene expression during the spontaneous differentiation using DNA microarray. As results of the microarray analysis using four human pluripotent stem cell lines, we found that some genes were commonly up- or down-regulated during the collapse of undifferentiated state. Furthermore, we analyzed the role of polycomb group during the collapse of undifferentiated state by ChIP-seq and ChIP-QPCR.

Research Products

• [Journal Article] Fluid dynamic forces created by rotary orbital suspension culture promote cardiomyogenic differentiation of human embryonic stem cells. (2013)
  • Author(s): Akimi Mogi, Shunsuke Takei, Hisashi Shimizu, Haruki Miura, Daihachiro Tomotsune*, and Katsunori Sasaki
  • Journal Title: J. Med. Biol. Eng Volume: (印刷中) Issue: 2 Pages: 101-101
  • DOI: 10.5405/jmbe.1356
  • Peer Reviewed
• [Presentation] GLOBAL ANALYSIS OF STABILITY OF UNDIFFERENTIATED STATE IN HUMAN PLURIPOTENT STEM CELLS (2013)
  • Author(s): Daihachiro Tomotsune, Akimi Mogi, Fengming Yue, Susumu Yoshie, Shoko Masuda, Ayana Sakurai, Daiki Fujita, Mika Morisaki-Nagai, Sakiko Takizawa-Shirasawa, Tadayuki Yokoyama, Katsunori Sasaki
  • Organizer: 第11回国際幹細胞学会(ISSCR)
  • Place of Presentation: ボストン(アメリカ合衆国)
  • Year and Date: 2013-06-12
• [Presentation] GLOBAL ANALYSIS OF STABILITY OF UNDIFFERENTIATED STATE IN HUMAN PLURIPOTENT STEM CELLS (2013)
  • Author(s): Daihachiro Tomotsune
  • Organizer: ISSCR（国際幹細胞学会）
  • Place of Presentation: アメリカ（ボストン）