Development of the Novel Nano Trench-Patterned Culture Surface for Initiating Differentiation of Mesenchymal Stem Cell
Project/Area Number |
22500430
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Waseda University |
Principal Investigator |
TAKEDA Naoya 早稲田大学, 理工学術院, 准教授 (60338978)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 電子線リソグラフィー / 細胞パターニング / 間葉系幹細胞 / 分化誘導 / 人工ニッチ / ナノ表面微細構造 / アストロサイト / 人工ニッシェ / ナノ表面構造 |
Research Abstract |
The novel cell patterning system was developed by utilizing the electron beam (EB) lithography and the EB-reactive polymer resists. And as a new method, we achieved inducing differentiation of mesechymal stem cell (MSC) just by culturing at the nano-topographic pattern of a single linear groove in 500 nm width, without transfecting any DNA and/or applying any biosignaling factor like concentional biological methods. MSCs were extraordinarily streched along the groove, presumably accompanying mechanical stress, and markedly increased gene expression of the neural markers and the osteogenic markers.
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Report
(4 results)
Research Products
(30 results)