Study on the sonodynamically induced antitumor effect of nano particles

• Project/Area Number: 22500444
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Yokohama College of Pharmacy
• Principal Investigator: YUMITA Nagahiko 横浜薬科大学, 薬学部, 准教授 (40191481)
• Co-Investigator(Kenkyū-buntansha): UMEMURA Shin-ichiro 東北大学, 工学部, 教授 (20402787) ; IWASE Yumiko 横浜薬科大学, 薬学部, 講師 (00521882)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 超音波 / 音響化学療法 / ナノ粒子 / フラーレン誘導体 / フラーレン / がん治療 / アポトーシス / 活性酸素種 / 一重項酸素

Research Abstract

The sonodynamically induced antitumor effect of fullerene derivatives such as pyrrolidine tris fullerenes (PTF) and polyhydroxy fullerenes (PF) was investigated. Both in vitro and in vivo antitumor effects were tested in combination with ultrasound at 2 MHz. The rate of ultrasonically induced damage on isolated sarcoma 180 cells in air-saturated suspension was enhanced by twice with80 〓M fullerene derivatives. This enhancement was significantly inhibited by histidine, which maysuggest it was mediated by ultrasonically induced oxidation. The coadministraion of 25 mg/kg fullerene derivatives followed by ultrasonic exposure at 2 MHz suppressed the growth of implanted colon 26 tomors at an intensity with which ultrasound alone showed only a slight antitumor effect.

Research Products

• [Journal Article] Apoptosis of HL-60 Cells Induced by Sonodynamic Therapy with Zinc Phthalocyanine Tetrasulfonate. (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda K, Onodera K, Fukai T, Umemura S, Momose Y.
  • Journal Title: Pharmacometrics Volume: 82 Pages: 75-83
  • NAID: 10030927893
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis by Novel Porphyrin Derivative,DCPH-P-Na(I). (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Komatsu H, Fukai T, Ikeda T, Umemura S, Momose Y.
  • Journal Title: Theranostics Volume: 2 Pages: 880-888
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis by4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-Porphynyl (IX)-6-7-diasparticacid (ATX-S10). (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda T, Fukai T, Onodera K, Takeda K, Umemura S, Okudaira K, Momose Y.
  • Journal Title: Biochemistry & AnalyticalBiochemistry Volume: 2 Pages: 2-7
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis Using a Novel Porphyrin Derivative (2012)
  • Author(s): Nagahiko Yumita, Yumiko Iwase, Koji Nishi, Hajime Komatsu, Kazuyoshi Takeda, Kenji Onodera, Toshio Fukai, Toshihiko Ikeda, Shin-ichiro Umemura, Kazuho Okudaira, Yasunori Momose
  • Journal Title: Theranostics Volume: 2 Issue: 9 Pages: 880-888
  • DOI: 10.7150/thno.3899
  • Peer Reviewed
• [Journal Article] Apoptosis of HL-60 Cells Induced by Sonodynamic Therapy with Zinc Phthalocyanine Tetrasulfonate (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda K, Onodera K, Fukai T, Umemura S, Momose Y.
  • Journal Title: Pharmacometrics, Volume: 82 Pages: 75-83
  • NAID: 10030927893
  • Peer Reviewed
• [Journal Article] Sonodynamically induced antitumor effect of mono-l-aspartyl chlorine6 (NPe6). (2011)
  • Author(s): Yumita, N., Iwase, Y., Nishi, K., Ikeda,T., Komatsu, H., Fukai, T., Onodera, K., Nishi, H., Takeda, K., Umemura, S., Okudaira, K., MomoseY.
  • Journal Title: Anticancer Res Volume: 31 Pages: 501-506
  • Peer Reviewed
• [Journal Article] Sonodynamic Antitumor Effect of Zinc Phthalocyanine Tetrasulfate on Murine SolidTumor colon 26 (2011)
  • Author(s): Yumita,N., Iwase, Y., Onodera, K. , Nishi, H., Takeda, K., Fukai, T., Ikeda, T., Umemura, S., Okudaira, K. , Momose Y
  • Journal Title: Pharmacometrics Volume: 80 Pages: 13-20
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Spe-cies in Sonodynami-cally Induced Apopto-sis by 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-Porphynyl (IX)-6-7-diaspartic acid (ATX-S10) (2011)
  • Author(s): N.Yumita, Y.Iwase, K.Nishi, T.Ikeda, T.Fukai, K.Takeda, K.Onodera, S.Umemura, K.Okudaira, Y.Momose
  • Journal Title: Biochemistry & Analytical Bio-chemistry Volume: S2 Issue: 02 Pages: 2-7
  • DOI: 10.4172/2161-1009.s2-001
  • Peer Reviewed
• [Journal Article] Sonodynamically induced antitumor effect of mono-l-aspartyl chlorin e6 (2011)
  • Author(s): N.Yumita
  • Journal Title: Anticancer Research Volume: 31 Pages: 501-506
  • Peer Reviewed
• [Journal Article] Effect of Zinc Phthaloctanine Tetrasulfate on Murine Solid Tumor colon 26 (2011)
  • Author(s): N.Yumita
  • Journal Title: Pharmacometrics Volume: 80 Pages: 13-20
  • Peer Reviewed
• [Journal Article] Sonodynamically Induced Cell Damage by Rose Bengal Derivative (2010)
  • Author(s): Sugita, N., Iwase, Y., Yumita, N., Ikeda, T., Umemura, S.
  • Journal Title: Anticancer Res Volume: 30 Pages: 3361-3366
  • Peer Reviewed
• [Journal Article] Construction of an Expression System for Human a1-Acid Glycoprotein in E. coli: the Roles of Oligosaccharide Moieties in Structural and Functional Properties, Drug Metab. (2010)
  • Author(s): Nishi, K., Fukunaga, N., Ono, T., Akuta, T., Yumita, N., Watanabe, H., Kadowaki, D., Suenaga, A., Maruyama, T., Otagiri, M.
  • Journal Title: Pharmacokinet Volume: 25 Pages: 200-207
  • Peer Reviewed
• [Journal Article] sonodynamicallyInduced Cell Damage andMembrane Lipid Peroxidation by Novel Porphyrin Derivative, DCPH-P-Na(I). (2010)
  • Author(s): Yumita, N., Iwase,Y., Nishi, K., Ikeda,T., Umemura, S., Sakata, I., Momose, Y.
  • Journal Title: Anticancer Res Volume: 30 Pages: 2241-2246
  • Peer Reviewed
• [Journal Article] Sonodynamically Induced apoptosis and active oxygen generation byATX-70 (2010)
  • Author(s): Yumita, N., Okudaira, K., Momose, Y., Umemura, S.
  • Journal Title: Cancer Chemother Pharmacol Volume: 66 Pages: 1078-1076
  • Peer Reviewed
• [Presentation] 新規ポルフィリン誘導体 ATXS-10 の音響化学的活性化によるアポトーシス誘導 (2012)
  • Author(s): 岩瀬由未子、弓田長彦、深井俊夫、武田収功、小野寺憲治、池田敏彦、梅村晋一郎、奥平和穂、百瀬弥寿徳
  • Organizer: 日本薬学会第132 年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-28
• [Presentation] Sonodynamically induced apoptosis by ATX-S10 in HL-60 cells (2011)
  • Author(s): Yumiko Iwase, Kazuho Okudaira, Shin-ichiro Umemura, Nagahiko Yumita
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] Sonodynamically-induced apoptosis by novel porphyrin derivative, ATX-S10 in HL-60 cells (2011)
  • Author(s): 岩瀬由未子、奥平和穂、梅村晋一郎、弓田長彦
  • Organizer: 日本癌学会第70回学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] 新規ポルフィリ ン誘導体 DCPH-P-Na (I) の音響化学的作用によるアポトーシス誘導 (2011)
  • Author(s): 岩瀬 由未子、今泉貴博、茅優華、桜沢藍、弓田長彦 、池田敏彦、深井俊夫、武田収功、小野寺憲治、西廣吉、奥平和穂、梅村晋一郎 、百瀬弥寿徳
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-28
• [Presentation] Sonodynamically-induced apoptosis by novel porphyrin derivative,DCPH in HL-60 cell (2010)
  • Author(s): 岩瀬 由未子、奥平和穂、梅村晋一郎、弓田長彦
  • Organizer: 日本癌学会第69回学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-28
• [Presentation] Sonodynamicaly-induced apoptosis by porphyrin novel derivative, DCPH in HL-60 cells. (2010)
  • Author(s): 岩瀬由未子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-23
• [Presentation] マイクロ バブルによるローズベンガル誘導体の音響化学的抗腫瘍効果の増強 (2010)
  • Author(s): 岩瀬由未子、杉田奈巳、弓田長彦、五十鈴川和人、西弘二、池田敏彦
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-30
• [Book] NEWパワーブック生物薬剤学第2版 (2011)
  • Author(s): 青木正忠、伊賀勝美、岩城正宏、金尾義治、九川文彦、櫻井栄一、田中哲郎、鍋倉智裕、三嶋基弘、村上照夫、村上正裕、森本一洋、山崎啓之、弓田長彦
  • Publisher: 廣川書店